Summary of Study ST000836

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000596. The data can be accessed directly via it's Project DOI: 10.21228/M8MX15 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000836
Study TitlePilot Human Muscle oral glucose tolerance test (OGTT)
Study TypeMS analysis
Study SummaryObesity is associated with metabolic inflexibility, the inability to switch from lipid to glucose oxidation during physiological conditions favoring excess glucose and insulin, which could contribute to the increased incidence of type 2 diabetes. Despite this knowledge, the role of skeletal muscle metabolites during these physiological conditions remain unclear and warrant further investigation. The purpose of this pilot study is to determine if: 1) there are differences in skeletal muscle glycolysis and TCA metabolites and amino acids between lean and obese individuals in the fasted state, and 2) if these metabolites change after 50 min of glucose ingestion, and 3) if there are differences in these metabolites between lean and obese individuals after 50 min of glucose ingestion.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups5
Total Subjects6
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8MX15
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000596
Project DOI:doi: 10.21228/M8MX15
Project Title:Lean Obese Human Glucose Metabolism
Project Type:MS analysis
Project Summary:Lean and obese skeletal muscle TCA plus response to OGTT
Institute:Ohio University
Department:Biomedical Sciences
Laboratory:Consitt Lab
Last Name:Consitt
First Name:Leslie
Address:Athens, Ohio
Email:consitt@ohio.edu
Phone:252-367-9228

Subject:

Subject ID:SU000863
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Body Comp Gender Timepoint
SA046621S00028064Lean Female 50min
SA046622S00028062Lean Female 50min
SA046623S00028069Lean Female Baseline
SA046624S00028061Lean Female Baseline
SA046625S00028063Lean Female Baseline
SA046626S00028068Obese Female 50min
SA046627S00028066Obese Female 50min
SA046628S00028065Obese Female Baseline
SA046629S00028070Obese Female Baseline
SA046630S00028067Obese Female Baseline
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000857
Collection Summary:-
Sample Type:Muscle

Treatment:

Treatment ID:TR000877
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000870
Sampleprep Summary:-
Sampleprep Protocol Filename:A043-TCA-Plus-20170713.pdf

Combined analysis:

Analysis ID AN001338 AN001339
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent Agilent
Column Phenomenex Luna NH2 (150 x 1mm,3um) Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6520B QTOF Agilent 6520B QTOF
Ion Mode NEGATIVE NEGATIVE
Units ISTD response ratio nmol/mg

Chromatography:

Chromatography ID:CH000935
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS001230
Analysis ID:AN001338
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A043-TCA-Plus-20170713.pdf
  
MS ID:MS001231
Analysis ID:AN001339
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A043-TCA-Plus-20170713.pdf
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