Summary of Study ST000837
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000597. The data can be accessed directly via it's Project DOI: 10.21228/M8H678 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000837 |
| Study Title | Hepatocyte Differentiation Metabolism |
| Study Type | MS analysis |
| Study Summary | In the first group of samples, we are looking into the different metabolites and their concentrations within our human embryonic stem cells over the course of differentiation. Our goal is to study how the metabolism of stem cells are changing as they undergo this conversion to become hepatocyte-like cells. In the second group of samples labeled HyCell, we would like to understand the concentrations of metabolites within our CHO cells in order to update the parameters for our metabolic model. Included are two samples from different days of a suspension batch culture which may have different lactate production qualities. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-08-02 |
| Num Groups | 9 |
| Total Subjects | 5 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000597 |
| Project DOI: | doi: 10.21228/M8H678 |
| Project Title: | Hepatocyte Metabolism |
| Project Type: | MS analysis |
| Project Summary: | Differentiating Stem Cells towards Hepatocytes |
| Institute: | University of Minnesota |
| Department: | Chemical Engineering |
| Laboratory: | Wei-Shou Hu Lab |
| Last Name: | Hu |
| First Name: | Wei-Shou |
| Address: | St. Paul, Minnesota |
| Email: | wshu@umn.edu |
| Phone: | 612-625-0546 |
Subject:
| Subject ID: | SU000864 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Cell Line | DAYS | Species |
|---|---|---|---|---|
| SA046632 | S00028164 | H9 | 10 | Homo sapiens |
| SA046633 | S00028163 | H9 | 2 | Homo sapiens |
| SA046631 | S00028162 | H9 | - | Homo sapiens |
| SA046634 | S00028165 | HyCell | 3 | Rodentia |
| SA046635 | S00028166 | HyCell | 4 | Rodentia |
| Showing results 1 to 5 of 5 |
Collection:
| Collection ID: | CO000858 |
| Collection Summary: | - |
| Sample Type: | Stem cells |
Treatment:
| Treatment ID: | TR000878 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000871 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A011_Glycolysis-TCA-nucleotides.pdf |
Combined analysis:
| Analysis ID | AN001340 | AN001341 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent | Agilent |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6520B QTOF | Agilent 6520B QTOF |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | pmol/ug protein | counts |
Chromatography:
| Chromatography ID: | CH000936 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH000937 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001232 |
| Analysis ID: | AN001340 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
| MS ID: | MS001233 |
| Analysis ID: | AN001341 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A043-TCA-Plus-20170713.pdf |
