Summary of Study ST000838
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000598. The data can be accessed directly via it's Project DOI: 10.21228/M8CD6Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000838 |
| Study Title | Obesity and marrow and marrow adipose tissue (MAT) metabolomics |
| Study Type | MS analysis |
| Study Summary | Male C57Bl6j mice were fed HFD for 16-20 weeks and marrow adipose tissue (MAT) and bone marrow collected to evaluate lipid content/lipid type. In addition, marrow evaluations for glycolysis/TCA cycle and acylcarnitines will be performed. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-08-02 |
| Num Groups | 4 |
| Total Subjects | 6 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-08-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000598 |
| Project DOI: | doi: 10.21228/M8CD6Q |
| Project Title: | Obesity and marrow and MAT metabolomics Studies |
| Project Type: | MS analysis |
| Project Summary: | MAT and bone marrow collected to evaluate lipid content/lipid type. In addition, marrow evaluations for glycolysis/TCA cycle and acylcarnitines will be performed. |
| Institute: | University of Michigan |
| Department: | Pedatric Endocrinology |
| Laboratory: | Singer Lab |
| Last Name: | Singer |
| First Name: | Kanakadurga |
| Address: | Ann Arbor, MI |
| Email: | ksinger@umich.edu |
| Phone: | 734-764-5175 |
Subject:
| Subject ID: | SU000865 |
| Subject Type: | MOUSE |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet | Sample type |
|---|---|---|---|
| SA046636 | S00028172 | HFD | Adipose Tissue |
| SA046637 | S00028170 | HFD | Adipose Tissue |
| SA046638 | S00028171 | HFD | Adipose Tissue |
| SA046639 | S00028177 | HFD | Bone Marrow |
| SA046640 | S00028176 | HFD | Bone Marrow |
| SA046641 | S00028178 | HFD | Bone Marrow |
| SA046642 | S00028167 | ND | Adipose Tissue |
| SA046643 | S00028169 | ND | Adipose Tissue |
| SA046644 | S00028168 | ND | Adipose Tissue |
| SA046645 | S00028173 | ND | Bone Marrow |
| SA046646 | S00028174 | ND | Bone Marrow |
| SA046647 | S00028175 | ND | Bone Marrow |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO000859 |
| Collection Summary: | - |
| Sample Type: | Bone marrow |
Treatment:
| Treatment ID: | TR000879 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000872 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A008_Acyl_Carnitines.pdf |
Combined analysis:
| Analysis ID | AN001342 | AN001343 | AN001344 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | HILIC | HILIC |
| Chromatography system | Agilent | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Phenomenex Luna NH2 (150 x 1mm,3um) | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | QTOF | QTOF |
| MS instrument name | Agilent 6490A QQQ | Agilent 6520B QTOF | Agilent 6520B QTOF |
| Ion Mode | POSITIVE | NEGATIVE | NEGATIVE |
| Units | uM | pmol/mg | counts/mg |
Chromatography:
| Chromatography ID: | CH000938 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.250 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000939 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH000940 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001234 |
| Analysis ID: | AN001342 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A008_-_Acyl_Carnitines.pdf |
| MS ID: | MS001235 |
| Analysis ID: | AN001343 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
| MS ID: | MS001236 |
| Analysis ID: | AN001344 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A043-TCA-Plus-20170713.pdf |
