Summary of Study ST000839
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000542. The data can be accessed directly via it's Project DOI: 10.21228/M8M96V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000839 |
| Study Title | Effect of cooling on muscle tissue metabolism (part II) |
| Study Type | MS analysis |
| Study Summary | Thermal modalities are commonly used in sports medicine to affect tissue healing. Cold therapy is commonly used modalities, but the metabolic changes to muscle after cooling are not known. The objective of this study is to look at the effect of cooling on muscle metabolites and gene expression. There are a total of 8 subjects in the study. Each subject had an ice cup cryotherapy treatment (cool samples) to one leg for 15 minutes, and the other leg served as the control (cntrl samples). Two hours after the application of cryotherapy, a biopsy was taken from each thigh muscle. Muscle was minced with scissors and quickly snap frozen in liquid nitrogen. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-08-02 |
| Num Groups | 10 |
| Total Subjects | 8 |
| Study Comments | We would like to run the TCA Plus Analysis on each of the samples. For the statistical analysis, we would normalize the cool side to the ctrl side, and look at the relative change in metabolite abundance as a result of the cooling procedure. only used in sports medicine to affect tissue healing. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-08-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000542 |
| Project DOI: | doi: 10.21228/M8M96V |
| Project Title: | Thermal Modalities Study |
| Project Type: | MS analysis |
| Project Summary: | This study is looking at the effect of cooling on muscle tissue metabolism |
| Institute: | University of Michigan |
| Department: | Orthopaedic Surgery |
| Laboratory: | Mendias Lab |
| Last Name: | Mendias |
| First Name: | Christopher |
| Address: | Ann Arbor, MI |
| Email: | cmendias@umich.edu |
| Phone: | 734-764-3250 |
Subject:
| Subject ID: | SU000866 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Temperature |
|---|---|---|
| SA046648 | S00023215 | Control |
| SA046649 | S00023225 | Control |
| SA046650 | S00023229 | Control |
| SA046651 | S00023221 | Control |
| SA046652 | S00023227 | Control |
| SA046653 | S00023223 | Control |
| SA046654 | S00023217 | Control |
| SA046655 | S00023219 | Control |
| SA046656 | S00023230 | Cooled |
| SA046657 | S00023216 | Cooled |
| SA046658 | S00023228 | Cooled |
| SA046659 | S00023226 | Cooled |
| SA046660 | S00023220 | Cooled |
| SA046661 | S00023224 | Cooled |
| SA046662 | S00023222 | Cooled |
| SA046663 | S00023218 | Cooled |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000860 |
| Collection Summary: | - |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR000880 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000873 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A011_Glycolysis-TCA-nucleotides.pdf |
Combined analysis:
| Analysis ID | AN001345 | AN001346 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent | Agilent |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6520B QTOF | Agilent 6520B QTOF |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | nmol/mg | counts |
Chromatography:
| Chromatography ID: | CH000941 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH000942 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001237 |
| Analysis ID: | AN001345 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
| MS ID: | MS001238 |
| Analysis ID: | AN001346 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A043-TCA-Plus-20170713.pdf |
