Summary of Study ST000840

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000509. The data can be accessed directly via it's Project DOI: 10.21228/M8VW91 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000840
Study TitleDiabetic heart adrenergic signaling and metabolism (STZ & Isoproterenol, High Fat) - part II
Study TypeMS analysis
Study SummaryWe are examing the effect of both diabetes and adrenergic activation on the metabolomic profile. STZ treatment was initiated 5 months before hearts were harvested. High fat/low fat diets were given for 13 weeks. All hearts were excised in the morning. For the STZ/isoproterenol experiment, mouse anesthesia was initiated with 4% isoflurane, and maintained with 2% and a homeothermic controller. After 5 min of equilibration, control and fasted mice were injected intraperitoneally with either saline (S) or 25ug/kg isoproterenol (I). After an additional 5 min, the whole heart was quickly excised, washed with ice-cold distilled water, dried off with a chem whipe, and flash frozen in liquid nitrogen. Hearts were stored at -80, and shipped using dry ice. The hearts from the high fat diet experiement were prepared in the same way, but no injection was given and total anesthesia time was 5min instead of 10min.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups7
Total Subjects29
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS/LC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8VW91
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000509
Project DOI:doi: 10.21228/M8VW91
Project Title:Diabetic Heart
Project Type:MS analysis
Project Summary:Diabetic heart adrenergic signaling and metabolism
Institute:Oklahoma Medical Research Foundation
Department:Aging & Metabolism
Laboratory:Humphries Lab
Last Name:Humphries
First Name:Kenneth
Address:Oklahoma City, OK
Email:Kenneth-Humphries@omrf.org
Phone:405-271-7584

Subject:

Subject ID:SU001181
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Type
SA077813S00022386Isoproterenol Control
SA077814S00022388Isoproterenol Control
SA077815S00022387Isoproterenol Control
SA077816S00022389Isoproterenol Control
SA077817S00022392Isoproterenol STZ diabetic
SA077818S00022394Isoproterenol STZ diabetic
SA077819S00022393Isoproterenol STZ diabetic
SA077820S00022391Isoproterenol STZ diabetic
SA077821S00022390Isoproterenol STZ diabetic
SA077832S00022400none High fat diet
SA077833S00022402none High fat diet
SA077834S00022404none High fat diet
SA077835S00022401none High fat diet
SA077836S00022403none High fat diet
SA077837S00022395none Low fat diet
SA077838S00022396none Low fat diet
SA077839S00022397none Low fat diet
SA077840S00022398none Low fat diet
SA077841S00022399none Low fat diet
SA077822S00022380Saline Control
SA077823S00022378Saline Control
SA077824S00022377Saline Control
SA077825S00022376Saline Control
SA077826S00022379Saline Control
SA077827S00022381Saline STZ diabetic
SA077828S00022384Saline STZ diabetic
SA077829S00022383Saline STZ diabetic
SA077830S00022385Saline STZ diabetic
SA077831S00022382Saline STZ diabetic
Showing results 1 to 29 of 29

Collection:

Collection ID:CO001175
Collection Summary:-
Sample Type:Heart

Treatment:

Treatment ID:TR001196
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP001189
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001844 AN001845
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent Agilent GC_7890N
Column Phenomenex Luna NH2 (150 x 1mm,3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6520B QTOF Agilent MS_5975
Ion Mode NEGATIVE POSITIVE
Units nmol/mg nmol/mg

Chromatography:

Chromatography ID:CH001321
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Flow Rate:0.075 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC
  
Chromatography ID:CH001322
Instrument Name:Agilent GC_7890N
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS001705
Analysis ID:AN001844
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:none
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm.m
Analysis Protocol File:A011_Glycolysis-TCA-nucleotides.pdf
  
MS ID:MS001706
Analysis ID:AN001845
Instrument Name:Agilent MS_5975
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:none
Ion Mode:POSITIVE
Acquisition Parameters File:ALPHA KETO ACIDS-FULL.M
Analysis Protocol File:A011_Glycolysis-TCA-nucleotides.pdf
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