Summary of Study ST000840
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000509. The data can be accessed directly via it's Project DOI: 10.21228/M8VW91 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000840 |
Study Title | Diabetic heart adrenergic signaling and metabolism (STZ & Isoproterenol, High Fat) - part II |
Study Type | MS analysis |
Study Summary | We are examing the effect of both diabetes and adrenergic activation on the metabolomic profile. STZ treatment was initiated 5 months before hearts were harvested. High fat/low fat diets were given for 13 weeks. All hearts were excised in the morning. For the STZ/isoproterenol experiment, mouse anesthesia was initiated with 4% isoflurane, and maintained with 2% and a homeothermic controller. After 5 min of equilibration, control and fasted mice were injected intraperitoneally with either saline (S) or 25ug/kg isoproterenol (I). After an additional 5 min, the whole heart was quickly excised, washed with ice-cold distilled water, dried off with a chem whipe, and flash frozen in liquid nitrogen. Hearts were stored at -80, and shipped using dry ice. The hearts from the high fat diet experiement were prepared in the same way, but no injection was given and total anesthesia time was 5min instead of 10min. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2017-08-02 |
Num Groups | 7 |
Total Subjects | 29 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2018-02-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000509 |
Project DOI: | doi: 10.21228/M8VW91 |
Project Title: | Diabetic Heart |
Project Type: | MS analysis |
Project Summary: | Diabetic heart adrenergic signaling and metabolism |
Institute: | Oklahoma Medical Research Foundation |
Department: | Aging & Metabolism |
Laboratory: | Humphries Lab |
Last Name: | Humphries |
First Name: | Kenneth |
Address: | Oklahoma City, OK |
Email: | Kenneth-Humphries@omrf.org |
Phone: | 405-271-7584 |
Subject:
Subject ID: | SU001181 |
Subject Type: | MOUSE |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment | Type |
---|---|---|---|
SA077813 | S00022386 | Isoproterenol | Control |
SA077814 | S00022388 | Isoproterenol | Control |
SA077815 | S00022387 | Isoproterenol | Control |
SA077816 | S00022389 | Isoproterenol | Control |
SA077817 | S00022392 | Isoproterenol | STZ diabetic |
SA077818 | S00022394 | Isoproterenol | STZ diabetic |
SA077819 | S00022393 | Isoproterenol | STZ diabetic |
SA077820 | S00022391 | Isoproterenol | STZ diabetic |
SA077821 | S00022390 | Isoproterenol | STZ diabetic |
SA077832 | S00022400 | none | High fat diet |
SA077833 | S00022402 | none | High fat diet |
SA077834 | S00022404 | none | High fat diet |
SA077835 | S00022401 | none | High fat diet |
SA077836 | S00022403 | none | High fat diet |
SA077837 | S00022395 | none | Low fat diet |
SA077838 | S00022396 | none | Low fat diet |
SA077839 | S00022397 | none | Low fat diet |
SA077840 | S00022398 | none | Low fat diet |
SA077841 | S00022399 | none | Low fat diet |
SA077822 | S00022380 | Saline | Control |
SA077823 | S00022378 | Saline | Control |
SA077824 | S00022377 | Saline | Control |
SA077825 | S00022376 | Saline | Control |
SA077826 | S00022379 | Saline | Control |
SA077827 | S00022381 | Saline | STZ diabetic |
SA077828 | S00022384 | Saline | STZ diabetic |
SA077829 | S00022383 | Saline | STZ diabetic |
SA077830 | S00022385 | Saline | STZ diabetic |
SA077831 | S00022382 | Saline | STZ diabetic |
Showing results 1 to 29 of 29 |
Collection:
Collection ID: | CO001175 |
Collection Summary: | - |
Sample Type: | Heart |
Treatment:
Treatment ID: | TR001196 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP001189 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | A011_Glycolysis-TCA-nucleotides.pdf |
Combined analysis:
Analysis ID | AN001844 | AN001845 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | GC |
Chromatography system | Agilent | Agilent GC_7890N |
Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
MS Type | ESI | EI |
MS instrument type | QTOF | Single quadrupole |
MS instrument name | Agilent 6520B QTOF | Agilent MS_5975 |
Ion Mode | NEGATIVE | POSITIVE |
Units | nmol/mg | nmol/mg |
Chromatography:
Chromatography ID: | CH001321 |
Instrument Name: | Agilent |
Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
Flow Rate: | 0.075 mL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
Chromatography ID: | CH001322 |
Instrument Name: | Agilent GC_7890N |
Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS001705 |
Analysis ID: | AN001844 |
Instrument Name: | Agilent 6520B QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | none |
Ion Mode: | NEGATIVE |
Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m |
Analysis Protocol File: | A011_Glycolysis-TCA-nucleotides.pdf |
MS ID: | MS001706 |
Analysis ID: | AN001845 |
Instrument Name: | Agilent MS_5975 |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | none |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | ALPHA KETO ACIDS-FULL.M |
Analysis Protocol File: | A011_Glycolysis-TCA-nucleotides.pdf |