Summary of Study ST000841

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000599. The data can be accessed directly via it's Project DOI: 10.21228/M87Q3F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000841
Study TitleMetabolomics of muscle in insulin sensitive and resistant obese indivduals.
Study TypeMS analysis
Study SummaryOC samples: muscle biopsy samples collected from insulin sensitive / insulin resistant obese indivduals before undergoing a hyperinsulinemic-euglycemic clamp. Subjects were previously categorized for the rate of fatty acid release from adipose tissue into the bloodstream as a measure of insuline resistance. Low-FA, Med-FA, and High-FA stand for low, medium, and high rate of release.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups4
Total Subjects28
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M87Q3F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000599
Project DOI:doi: 10.21228/M87Q3F
Project Title:Prelim data for NIH exercise consortium
Project Type:MS analysis
Project Summary:Plasma samples before and after exercise training in obese adults
Institute:University of Michigan
Department:Kinesiology
Laboratory:Horowitz Lab
Last Name:Horowitz
First Name:Jeff
Address:Ann Arbor, MI
Email:jeffhoro@umich.edu
Phone:734-647-3354

Subject:

Subject ID:SU000868
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Subject group
SA046693S00020440HIGH-FA
SA046694S00020450HIGH-FA
SA046695S00020451HIGH-FA
SA046696S00020449HIGH-FA
SA046697S00020452HIGH-FA
SA046698S00020447HIGH-FA
SA046699S00020445HIGH-FA
SA046700S00020448HIGH-FA
SA046701S00020439HIGH-FA
SA046702S00020427LOW-FA
SA046703S00020442LOW-FA
SA046704S00020436LOW-FA
SA046705S00020430LOW-FA
SA046706S00020428LOW-FA
SA046707S00020431LOW-FA
SA046708S00020433LOW-FA
SA046709S00020434LOW-FA
SA046710S00020446MID-FA
SA046711S00020441MID-FA
SA046712S00020432MID-FA
SA046713S00020429MID-FA
SA046714S00020444MID-FA
SA046715S00020435MID-FA
SA046716S00020437MID-FA
SA046717S00020438MID-FA
SA046718S00020443MID-FA
SA046719S00020453N/A
SA046720S00020454N/A
Showing results 1 to 28 of 28

Collection:

Collection ID:CO000862
Collection Summary:-
Sample Type:Muscle

Treatment:

Treatment ID:TR000882
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000875
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001349 AN001350 AN001351 AN001352 AN001353 AN001354 AN001355
Analysis type MS MS MS MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase Reversed phase HILIC HILIC
Chromatography system Agilent Agilent Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2 Agilent Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um) Phenomenex Luna NH2 (150 x 1mm,3um) Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI ESI ESI ESI ESI ESI ESI
MS instrument type QTOF QTOF Triple TOF Triple TOF Triple quadrupole QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF Agilent 6490A QQQ Agilent 6520B QTOF Agilent 6520B QTOF
Ion Mode NEGATIVE POSITIVE NEGATIVE POSITIVE POSITIVE NEGATIVE NEGATIVE
Units counts Counts counts norm counts norm pmol/mg counts ug/100 mg

Chromatography:

Chromatography ID:CH000944
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000945
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH000946
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Rate:0.250 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase
  
Chromatography ID:CH000947
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Flow Rate:0.075 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS001241
Analysis ID:AN001349
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001242
Analysis ID:AN001350
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001243
Analysis ID:AN001351
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS001244
Analysis ID:AN001352
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS001245
Analysis ID:AN001353
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A008_-_Acyl_Carnitines.pdf
  
MS ID:MS001246
Analysis ID:AN001354
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A011_-_Glycolysis-TCA-nucleotides.pdf
  
MS ID:MS001247
Analysis ID:AN001355
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A011_-_Glycolysis-TCA-nucleotides.pdf
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