Summary of Study ST000873

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000604. The data can be accessed directly via it's Project DOI: 10.21228/M8M09W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000873
Study TitleMaternal Hypoxemia and oxidative stress in the fetus, newborn, and adult. exercise training for peripheral artery disease (part II)
Study TypeDisease model
Study SummaryGestational hypoxia presents a significant stress to an unborn fetus that can lead to significant complications related to fetal growth restriction and resulting in diseases in the newborn as well as those manifesting later in life. Recent evidence indicates that inflammation and oxidative stress are contributing factors to hypoxia-related diseases. The Center for Perinatal Biology at Loma Linda University has studied gestational chronic hypoxia in a sheep model for over 20 years to study dysfunction of vascular and nonvascular tissues derived from mothers, fetuses and offspring. In this project we are attempting to use metabolomics to assess metabolic dysregulation in vascular tissues along with markers of oxidative stress and inflammation in the mother and offspring to determine the extent of dysregulation due to chronic hypoxia. Untargeted metabolomics analysis focused on sheep plasma and arteries from the lung, resistance arteries in the brain, uterine arteries, and cultured human myocytes will be used to explore markers of glucose and lipid metabolism disruption. Targeted analyses of oxylipins and endocannabinoids will be used on the same samples to explore markers of oxidative stress and inflammation, which should be increased during hypoxia. This study should delineate pathways and biomarkers that help explain how hypoxia leads to the development of neonatal as well as adult-onset diseases associated with chronic hypoxia that are inter-related with fetal growth restriction.
Institute
University of California, Davis
DepartmentUSDA Western Human Nutrition Research Center
LaboratoryNewman's Lab
Last NameNewman
First NameJohn
Address430 West Health Sciences Dr. Davis, Ca, 95616
EmailJohn.Newman@ars.usda.gov
Phone(530) 752-1009
Submit Date2017-08-30
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2017-10-11
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M8M09W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000604
Project DOI:doi: 10.21228/M8M09W
Project Title:Maternal Hypoxemia and oxidative stress in the fetus, newborn, and adult.
Project Summary:Gestational hypoxia presents a significant stress to an unborn fetus that can lead to significant complications related to fetal growth restriction and resulting in diseases in the newborn as well as those manifesting later in life. Recent evidence indicates that inflammation and oxidative stress are contributing factors to hypoxia-related diseases. The Center for Perinatal Biology at Loma Linda University has studied gestational chronic hypoxia in a sheep model for over 20 years to study dysfunction of vascular and nonvascular tissues derived from mothers, fetuses and offspring. In this project we are attempting to use metabolomics to assess metabolic dysregulation in vascular tissues along with markers of oxidative stress and inflammation in the mother and offspring to determine the extent of dysregulation due to chronic hypoxia. Untargeted metabolomics analysis focused on sheep plasma and arteries from the lung, resistance arteries in the brain, uterine arteries, and cultured human myocytes will be used to explore markers of glucose and lipid metabolism disruption. Targeted analyses of oxylipins and endocannabinoids will be used on the same samples to explore markers of oxidative stress and inflammation, which should be increased during hypoxia. This study should delineate pathways and biomarkers that help explain how hypoxia leads to the development of neonatal as well as adult-onset diseases associated with chronic hypoxia that are inter-related with fetal growth restriction.
Institute:University of California, Davis
Department:Genome and Biomedical Sciences Facility
Laboratory:WCMC Metabolomics Core
Last Name:Fiehn
First Name:Oliver
Address:1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Email:ofiehn@ucdavis.edu
Phone:(530) 754-8258
Funding Source:NIH U24DK097154

Subject:

Subject ID:SU000947
Subject Type:Sheep
Subject Species:Ovis aries
Taxonomy ID:9940
Gender:Both

Factors:

Subject type: Sheep; Subject species: Ovis aries (Factor headings shown in green)

mb_sample_id local_sample_id Tissue type Treatment group Life stage
SA053311S49Adipose Hypoxic Fetal
SA053312S48Adipose Hypoxic Fetal
SA053313S50Adipose Hypoxic Fetal
SA053314S51Adipose Hypoxic Fetal
SA053315S52Adipose Hypoxic Fetal
SA053316S47Adipose Hypoxic Fetal
SA053317S53Adipose Hypoxic Fetal
SA053318S60Adipose Hypoxic New Born
SA053319S61Adipose Hypoxic New Born
SA053320S63Adipose Hypoxic New Born
SA053321S64Adipose Hypoxic New Born
SA053322S59Adipose Hypoxic New Born
SA053323S62Adipose Hypoxic New Born
SA053324S45Adipose Normoxic Fetal
SA053325S42Adipose Normoxic Fetal
SA053326S46Adipose Normoxic Fetal
SA053327S41Adipose Normoxic Fetal
SA053328S43Adipose Normoxic Fetal
SA053329S44Adipose Normoxic Fetal
SA053330S57Adipose Normoxic New Born
SA053331S58Adipose Normoxic New Born
SA053332S54Adipose Normoxic New Born
SA053333S55Adipose Normoxic New Born
SA053334S56Adipose Normoxic New Born
SA053335S23Carotid Hypoxic Adult non-pregnant
SA053336S21Carotid Hypoxic Adult non-pregnant
SA053337S24Carotid Hypoxic Adult non-pregnant
SA053338S107Carotid Hypoxic Adult non-pregnant
SA053339S25Carotid Hypoxic Adult non-pregnant
SA053340S26Carotid Hypoxic Fetal
SA053341S28Carotid Hypoxic Fetal
SA053342S30Carotid Hypoxic Fetal
SA053343S27Carotid Hypoxic Fetal
SA053344S29Carotid Hypoxic Fetal
SA053345S35Carotid Normoxic Adult non-pregnant
SA053346S31Carotid Normoxic Adult non-pregnant
SA053347S33Carotid Normoxic Adult non-pregnant
SA053348S32Carotid Normoxic Adult non-pregnant
SA053349S34Carotid Normoxic Adult non-pregnant
SA053350S37Carotid Normoxic Fetal
SA053351S36Carotid Normoxic Fetal
SA053352S38Carotid Normoxic Fetal
SA053353S40Carotid Normoxic Fetal
SA053354S39Carotid Normoxic Fetal
SA053355S68Pulmonary Arteries Hypoxic Adult non-pregnant
SA053356S65Pulmonary Arteries Hypoxic Adult non-pregnant
SA053357S67Pulmonary Arteries Hypoxic Adult non-pregnant
SA053358S66Pulmonary Arteries Hypoxic Adult non-pregnant
SA053359S100Pulmonary Arteries Hypoxic Adult non-pregnant
SA053360S98Pulmonary Arteries Hypoxic Adult non-pregnant
SA053361S99Pulmonary Arteries Hypoxic Adult non-pregnant
SA053362S88Pulmonary Arteries Hypoxic Fetal
SA053363S103Pulmonary Arteries Hypoxic Fetal
SA053364S83Pulmonary Arteries Hypoxic Fetal
SA053365S84Pulmonary Arteries Hypoxic Fetal
SA053366S87Pulmonary Arteries Hypoxic Fetal
SA053367S85Pulmonary Arteries Hypoxic Fetal
SA053368S74Pulmonary Arteries Hypoxic New Born
SA053369S97Pulmonary Arteries Hypoxic New Born
SA053370S96Pulmonary Arteries Hypoxic New Born
SA053371S101Pulmonary Arteries Normoxic Adult non-pregnant
SA053372S79Pulmonary Arteries Normoxic Adult non-pregnant
SA053373S80Pulmonary Arteries Normoxic Adult non-pregnant
SA053374S81Pulmonary Arteries Normoxic Adult non-pregnant
SA053375S82Pulmonary Arteries Normoxic Adult non-pregnant
SA053376S102Pulmonary Arteries Normoxic Adult non-pregnant
SA053377S104Pulmonary Arteries Normoxic Fetal
SA053378S91Pulmonary Arteries Normoxic Fetal
SA053379S89Pulmonary Arteries Normoxic Fetal
SA053380S93Pulmonary Arteries Normoxic Fetal
SA053381S105Pulmonary Arteries Normoxic Fetal
SA053382S106Pulmonary Arteries Normoxic Fetal
SA053383S72Pulmonary Arteries Normoxic New Born
SA053384S95Pulmonary Arteries Normoxic New Born
SA053385S71Pulmonary Arteries Normoxic New Born
SA053386S13Uterine Arteries Hypoxic Adult non-pregnant
SA053387S11Uterine Arteries Hypoxic Adult non-pregnant
SA053388S15Uterine Arteries Hypoxic Adult non-pregnant
SA053389S12Uterine Arteries Hypoxic Adult non-pregnant
SA053390S14Uterine Arteries Hypoxic Adult non-pregnant
SA053391S16Uterine Arteries Hypoxic Adult pregnant
SA053392S18Uterine Arteries Hypoxic Adult pregnant
SA053393S20Uterine Arteries Hypoxic Adult pregnant
SA053394S17Uterine Arteries Hypoxic Adult pregnant
SA053395S19Uterine Arteries Hypoxic Adult pregnant
SA053396S5Uterine Arteries Normoxic Adult non-pregnant
SA053397S1Uterine Arteries Normoxic Adult non-pregnant
SA053398S2Uterine Arteries Normoxic Adult non-pregnant
SA053399S3Uterine Arteries Normoxic Adult non-pregnant
SA053400S4Uterine Arteries Normoxic Adult non-pregnant
SA053401S7Uterine Arteries Normoxic Adult pregnant
SA053402S6Uterine Arteries Normoxic Adult pregnant
SA053403S8Uterine Arteries Normoxic Adult pregnant
SA053404S9Uterine Arteries Normoxic Adult pregnant
SA053405S10Uterine Arteries Normoxic Adult pregnant
Showing results 1 to 95 of 95

Collection:

Collection ID:CO000941
Collection Summary:Part of the routinely flash frozen samples from the Loma Linda University School of Medicine, Center for Perinatal Biology.
Sample Type:Tissue
Tissue Cell Identification:Adipose tissue, Carotid, pulmonary arteries, uterine arteries

Treatment:

Treatment ID:TR000961
Treatment Summary:To induce chronic hypoxia, pregnant and non-pregnant ewes were transported to the White Mountain Research Station that is owned and operated by the University of California. Animals were housed at the Barcroft Research Station (3800 m) for 100+ days prior to having the pregnant or non-pregnant ewes or 2 week old newborn lambs transported back to Loma Linda for study.

Sample Preparation:

Sampleprep ID:SP000954
Sampleprep Summary:After sample randomization, weighed tissue (around 10mg for adipose and 50mg for arteries) in 2 mL polypropylene Eppendorf tube was enriched with deuterated surrogates in 20µL methanol (Tables S1 and S2 from Agrawal, K., L.A. Hassoun, N. Foolad, T.L. Pedersen, R.K. Sivamani, J.W. Newman. 2017. Sweat lipid mediator profiling: a non-invasive approach for cutaneous research. J. Lipid Res. 58:188–195 [EPub: Nov 7, 2016]. doi: 10.1194/jlr.M071738) and 5 μl of BHT/EDTA in 1:1 methanol/water (v/v). A total of 400 μl 1-cyclohexyl uredio, 3-dodecanoic acid / 1-phenyl ureido, 3-hexanoic acid (CUDA / PUHA) in 1:1 methanol/acetonitrile (v/v) was added, the sample ground with 3mm stainless steel beads (two for adipose tissue and 3 for blood vessel tissue) and agitation for 6 min at 1500RPM. Protein precipitate and debris were removed by centrifugation (10 min, 10,000 RCF, 6 °C). The supernatant was filtered by centrifugation through 1 µm PVDF membranes (Millipore, Billerica, MA) at 6 °C and 4,500 RCF for 3 min. The filtrate was stored in glass vials at -20 °C until UPLC-MS/MS analysis.

Combined analysis:

Analysis ID AN001478 AN001479
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Aquity C18 BEH (100 x 2.1mm,1.7um) Aquity C18 BEH (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 6500 QTrap ABI Sciex 6500 QTrap
Ion Mode NEGATIVE POSITIVE
Units Concentration (nM) Concentration (nM)

Chromatography:

Chromatography ID:CH001037
Instrument Name:Waters Acquity
Column Name:Aquity C18 BEH (100 x 2.1mm,1.7um)
Column Temperature:60 °C
Flow Gradient:See protocol/methods file
Flow Rate:0.25 mL/min
Internal Standard:See protocol/methods file
Retention Time:See protocol/methods file
Sample Injection:5 µL
Solvent A:100% water; 0.1% acetic acid
Solvent B:90% acetonitrile/ 10% isopropanol
Analytical Time:20 min
Weak Wash Solvent Name:20% methanol, 10% isopropanol
Weak Wash Volume:600 µL
Strong Wash Solvent Name:50:50 Acetonitrile:Methanol
Strong Wash Volume:600 µL
Chromatography Type:Reversed phase

MS:

MS ID:MS001362
Analysis ID:AN001478
Instrument Name:ABI Sciex 6500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001363
Analysis ID:AN001479
Instrument Name:ABI Sciex 6500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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