Summary of Study ST000902
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000461. The data can be accessed directly via it's Project DOI: 10.21228/M8G609 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000902 |
| Study Title | Omega-6 and omega-3 oxylipins are implicated in soybean oil-induced obesity in mice - Liver (part VI) |
| Study Summary | Characterization and analysis of metabolomic, proteomic and metabolic profiles of C57/Bl6N mice fed various high fat diets |
| Institute | University of California, Davis |
| Last Name | Yang |
| First Name | Jun |
| Address | 207 Everson Hall, Shields One Ave, Davis CA 95616 |
| junyang@ucdavis.edu | |
| Phone | 5307525109 |
| Submit Date | 2017-07-26 |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-11-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000461 |
| Project DOI: | doi: 10.21228/M8G609 |
| Project Title: | Omega-6 and omega-3 oxylipins are implicated in soybean oil-induced obesity in mice |
| Project Summary: | In this study we have compared the metabolic effects of conventional soybean oil to those of genetically modified Plenish soybean oil, that is low in linoleic acid and high in oleic acid. This work builds on our previous study showing that soybean oil, rich in polyunsaturated fats, is more obesogenic and diabetogenic than coconut oil, rich in saturated fats (PMID: 26200659). Here, in order to elucidate the mechanisms responsible for soybean oil induced obesity, we have performed the first ever metabolomics (in plasma and liver) and proteomics on the livers of mice fed the two soybean oil diets (plus those fed a high coconut oil and Viv chow diet). Our results show that the new high oleic soybean oil induces less obesity and adiposity than conventional soybean oil, but can cause hepatomegaly and liver dysfunction. Metabolomic analysis reveals that the hepatic and plasma metabolic profiles differ considerably between the two soybean oils. Hepatic C18 oxylipin metabolites of omega-6 (ω6) and omega-3 (ω3) fatty acids (linoleic and α-linolenic acid, respectively) in the cytochrome P450/soluble epoxide hydrolase pathway were found to correlate positively with obesity. |
| Institute: | University of California, Riverside |
| Department: | Cell Biology and Neuroscience |
| Last Name: | Sladek |
| First Name: | Frances |
| Address: | 2115 Biological Sciences Building,University of California, Riverside, CA 92521-0314 |
| Email: | frances.sladek@ucr.edu |
| Phone: | 951-827-2264 |
Subject:
| Subject ID: | SU000939 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet |
|---|---|---|
| SA052906 | PM D4-18 e2 | HFD 24 |
| SA052907 | PM D4-18 e4 | HFD 24 |
| SA052908 | PM D4-18 e1 | HFD 24 |
| SA052909 | PM D4-16 e4 | HFD 24 |
| SA052910 | PM D4-16 e1 | HFD 24 |
| SA052911 | PM D3-18 e1 | LA-HFD 24 |
| SA052912 | PM D3-18 e4 | LA-HFD 24 |
| SA052913 | PM D3-17 e4 | LA-HFD 24 |
| SA052914 | PM D3-17 e3 | LA-HFD 24 |
| SA052915 | PM D3-17 e2 | LA-HFD 24 |
| SA052916 | PM PL-4 e4 | PL_HFD_24 |
| SA052917 | PM PL-5 e2 | PL_HFD_24 |
| SA052918 | PM PL-4 e3 | PL_HFD_24 |
| SA052919 | PM PL-4 e2 | PL_HFD_24 |
| SA052920 | PM PL-4 e1 | PL_HFD_24 |
| SA052921 | CR Viv-1 24 wk -2 | Viv_24 |
| SA052922 | CR Viv-1 24 wk -3 | Viv_24 |
| SA052923 | CR Viv-1 24 wk -1 | Viv_24 |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO000933 |
| Collection Summary: | Liver tissue from mice on the diets for 24 weeks for metabolomic analysis was collected, rinsed in cold PBS, excess fluid was blotted with a kim-wipe and tissue was immediately snap frozen in liquid nitrogen before storage at -80°C. Blood was collected by cardiac puncture and centrifuged at 9 rcf for 5 min at 4°C. Plasma was stored immediately at -20°C. |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR000953 |
| Treatment Summary: | Male C57/BL6N mice weaned at 3weeks of age were randomly assigned to one of the four diets-Viv chow, 40 kcal% coconut oil , 40 kcal% total fat soybean oil diet(21 kcal% from coconut oil and 19 kcal% from soybean oil) or 40 kcal% total fat. Plenish oil diet (21 kcal% from coconut oil and 19 kcal% from Plenish oil) for 24 weeks. |
Sample Preparation:
| Sampleprep ID: | SP000946 |
| Sampleprep Summary: | Preparation of SPE on vacuum manifold: 1. Clean 60 mg Oasis HLB (Waters) spe column with 1 column volume ethyl acetate followed by 2 column volumes MeOH 2. Condition column with 2 column volume 0.1% acetic acid :5% MeOH solution 3. Close valves tightly when meniscus reaches frit over sorbent bed. Sample extraction by SPE on vacuum manifold: 4. If not in sample at collection, add 10 µL of 0.2 mg/mL of EDTA and BHT in MeOH: H2O (1:1) to sorbent bed of all columns 5. Vortex each sample a few seconds to homogenize just prior to loading. 6. Load 250µL plasma sample into SPE column. 7. Spike each column with 10µl 500nM surrogate solution 8. Load 1.5ml of 0.1% acetic acid: 5% MeOH solution to each column and let sit a few minutes. 9. Open valves to extract by gravity (use low vacuum only if necessary) 10. Wash SPE with 2 column volumes 0.1% acetic acid :5% MeOH solution 11. Pull aqueous plug from SPE with a few seconds high vacuum. 12. Further dry SPE with low vacuum. ~20min, -20psi. 13. For each sample, spike 2ml eppendorf tube with 6µl 30% glycerol in MeOH prior to eluate collection. 14. Elute spe into tube with 0.5 ml methanol followed by 1.5 mL of ethyl acetate 15. Evaporate extract until 2 µl glycerol plug is left, by Speed-vac. 16. Cap and freeze by liquid nitrogen (for shipping) or store at -80ºC until analysis. |
Combined analysis:
| Analysis ID | AN001468 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1200SL |
| Column | Agilent Eclipse Plus C18 (150 x 2.1mm, 1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 4000 QTrap |
| Ion Mode | NEGATIVE |
| Units | Concentration pmol/L |
Chromatography:
| Chromatography ID: | CH001032 |
| Instrument Name: | Agilent 1200SL |
| Column Name: | Agilent Eclipse Plus C18 (150 x 2.1mm, 1.8um) |
| Flow Rate: | 0.35 mL/min |
| Sample Injection: | 10 uL |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001356 |
| Analysis ID: | AN001468 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Ion Source Temperature: | 600 C |
