Summary of Study ST000927

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000642. The data can be accessed directly via it's Project DOI: 10.21228/M8PM4T This work is supported by NIH grant, U2C- DK119886.


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Study IDST000927
Study TitleProbing the metabolic phenotype of breast cancer cells by multiple tracer stable isotope resolved metabolomics (part II)
Study SummaryBreast cancers vary by their origin and specific set of genetic lesions, which gives rise to distinct phenotypes and differential response to targeted and untargeted chemotherapies. To explore the functional differences of different breast cell types, we performed Stable Isotope Resolved Metabolomics (SIRM) studies of one primary breast (HMEC) and three breast cancer cells (MCF-7, MDAMB-231, and ZR75-1) having distinct genotypes and growth characteristics, using 13C6-glucose, 13C-1+2-glucose, 13C5,15N2-Gln, 13C3-glycerol, and 13C8-octanoate as tracers. These tracers were designed to probe the central energy producing and anabolic pathways (glycolysis, pentose phosphate pathway, Krebs Cycle, glutaminolysis, nucleotide synthesis and lipid turnover). We found that glycolysis was not associated with the rate of breast cancer cell proliferation, glutaminolysis did not support lipid synthesis in primary breast or breast cancer cells, but was a major contributor to pyrimidine ring synthesis in all cell types; anaplerotic pyruvate carboxylation was activated in breast cancer versus primary cells. We also found that glucose metabolism in individual breast cancer cell lines differed between in vitro cultures and tumor xenografts, but not the metabolic distinctions between cell lines, which may reflect the influence of tumor architecture/microenvironment.
University of Kentucky
Last NameLane
First NameAndrew
AddressRm 516 Biopharm Complex, 789 S. Limestone St.,Univ. of Kentucky, Lexington, KY 40536
Submit Date2018-01-24
Analysis Type DetailNMR
Release Date2018-06-05
Release Version1
Andrew Lane Andrew Lane application/zip

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Project ID:PR000642
Project DOI:doi: 10.21228/M8PM4T
Project Title:Breast Cancer Metabolism
Project Summary:The program comprises three project areas utilizing stable isotope resolved metabolomics to gain a mechanistic understanding of NSCLC in situ. The projects combine cell culture, animal models and human subjects to define the influence of the tumor microenvironment on cancer progression.
Institute:University of Kentucky
Last Name:Lane
First Name:Andrew
Address:Rm 516 Biopharm Complex, 789 S. Limestone St.,Univ. of Kentucky, Lexington, KY 40536


Subject ID:SU000965
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606


Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell Line Tracer
SA055350HMEC_UC13Glc_Ctl_toc50HMEC Uniformly Labelled Glucose
SA055351HMEC_C13Gln_Ctl_toc50HMEC Uniformly Labelled Glutamine
SA055352MCF7_UC13Glc_Ctl_toc50MCF-7 Uniformly Labelled Glucose
SA055353MCF7_C13Gln_Ctl_toc50MCF-7 Uniformly Labelled Glutamine
SA055354ZR75_1_cells_24h_c13glcSep09_toc50ZR-75-1 Uniformly Labelled Glucose
SA055355ZR751_Nor_HiGlc_U13C15NGln_TOCSYZR-75-1 Uniformly Labelled Glutamine
Showing results 1 to 6 of 6


Collection ID:CO000959
Collection Summary:Cells were cultured in various media then harvested after 24 hours.
Sample Type:Cells


Treatment ID:TR000979
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000972
Sampleprep Summary:Cell lines are seperated into polar, non-polar, protein, and media.
Sampleprep Protocol Filename:Quench_Cell_Tissue_Fan03252015.pdf


Analysis ID:AN001520
Analysis Type:NMR
Num Factors:6
Num Metabolites:7


NMR ID:NM000117
Analysis ID:AN001520
Instrument Name:INOVA
Instrument Type:CW-NMR
NMR Experiment Type:Other
Spectrometer Frequency:800 MHz