Summary of Study ST000931

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000931
Study TitleActivity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv3915 (CwlM)
Study Typetimecourse
Study SummaryThese experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.
Institute
Weill Cornell Medicine
Last NameRhee
First NameKyu
AddressDepartment of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY
Emailkyr9001@med.cornell.edu
Phone979-229-1494
Submit Date2018-03-28
Analysis Type DetailLC-MS
Release Date2018-04-10
Release Version1
Kyu Rhee Kyu Rhee
https://dx.doi.org/10.21228/M8388M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000187
Project DOI:doi: 10.21228/M8388M
Project Title:FLUTE: Functionalizing Lists of Unknown TB Entities
Project Type:NIH U19
Project Summary:functional genomics of proteins of unknown function in M. tuberculosis
Institute:Weill Cornell Medicine
Department:Microbiology and Immunology
Last Name:Rhee
First Name:Kyu
Address:#N/A
Email:kyr9001@med.cornell.edu

Subject:

Subject ID:SU000970
Subject Type:Bacteria
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332

Factors:

Subject type: Bacteria; Subject species: Mycobacterium tuberculosis H37Rv (Factor headings shown in green)

mb_sample_id local_sample_id timepoint (min) protein prep
SA055536CwlM_B_1515 Active
SA055537CwlM_A_1515 Active
SA055538CwlM_C_1515 Active
SA055539Inactivated-CwlM_C_1515 Inactive
SA055540Inactivated-CwlM_B_1515 Inactive
SA055541Inactivated-CwlM_A_1515 Inactive
SA055542CwlM_C_3030 Active
SA055543CwlM_B_3030 Active
SA055544CwlM_A_3030 Active
SA055545Inactivated-CwlM_C_3030 Inactive
SA055546Inactivated-CwlM_A_3030 Inactive
SA055547Inactivated-CwlM_B_3030 Inactive
SA055530CwlM_A_0- Active
SA055531CwlM_C_0- Active
SA055532CwlM_B_0- Active
SA055533Inactivated-CwlM_B_0- Inactive
SA055534Inactivated-CwlM_C_0- Inactive
SA055535Inactivated-CwlM_A_0- Inactive
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000964
Collection Summary:Rv3915 from M. tuberculosis H37Rv was expressed in E. coli cells and purified.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000984
Treatment Summary:Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated).

Sample Preparation:

Sampleprep ID:SP000977
Sampleprep Summary:Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated).

Combined analysis:

Analysis ID AN001525 AN001526
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Agilent 1290
Column Microsolv Diamond hydride (150mm,4um) Microsolv Diamond hydride (150mm,4um)
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode POSITIVE NEGATIVE
Units integrated peak counts integrated peak counts

Chromatography:

Chromatography ID:CH001075
Chromatography Summary:Aqueous normal phase diamond hydride
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Chromatography Type:Normal phase

MS:

MS ID:MS001405
Analysis ID:AN001525
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001406
Analysis ID:AN001526
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:NEGATIVE
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