Summary of Study ST000943

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000943
Study TitleActivity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv3916c
Study Typetimecourse
Study SummaryThese experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.
Institute
Weill Cornell Medicine
Last NameRhee
First NameKyu
AddressDepartment of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY
Emailkyr9001@med.cornell.edu
Phone979-229-1494
Submit Date2018-03-07
Analysis Type DetailLC-MS
Release Date2018-04-10
Release Version1
Kyu Rhee Kyu Rhee
https://dx.doi.org/10.21228/M8388M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000187
Project DOI:doi: 10.21228/M8388M
Project Title:FLUTE: Functionalizing Lists of Unknown TB Entities
Project Type:NIH U19
Project Summary:functional genomics of proteins of unknown function in M. tuberculosis
Institute:Weill Cornell Medicine
Department:Microbiology and Immunology
Last Name:Rhee
First Name:Kyu
Address:#N/A
Email:kyr9001@med.cornell.edu

Subject:

Subject ID:SU000982
Subject Type:Bacteria
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332
Species Group:Bacteria

Factors:

Subject type: Bacteria; Subject species: Mycobacterium tuberculosis H37Rv (Factor headings shown in green)

mb_sample_id local_sample_id Time Protein
SA0559673916_B_1515 Active
SA0559683916_A_1515 Active
SA0559693916_C_1515 Active
SA055970Inactivated-3916_C_1515 Inactivated
SA055971Inactivated-3916_B_1515 Inactivated
SA055972Inactivated-3916_A_1515 Inactivated
SA0559733916_C_3030 Active
SA0559743916_B_3030 Active
SA0559753916_A_3030 Active
SA055976Inactivated-3916_C_3030 Inactivated
SA055977Inactivated-3916_A_3030 Inactivated
SA055978Inactivated-3916_B_3030 Inactivated
SA0559613916_A_0- Active
SA0559623916_C_0- Active
SA0559633916_B_0- Active
SA055964Inactivated-3916_B_0- Inactivated
SA055965Inactivated-3916_C_0- Inactivated
SA055966Inactivated-3916_A_0- Inactivated
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000976
Collection Summary:Rv3916c from M. tuberculosis H37Rv was expressed in E. coli cells and purified.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000996
Treatment Summary:Protein was incubated with whole-cell lysate in either Active form in Inactive (heat-inactivated).

Sample Preparation:

Sampleprep ID:SP000989
Sampleprep Summary:Protein was incubated with whole-cell lysate in either Active form or Inactive (heat-inactivated).

Chromatography:

Chromatography ID:CH001086
Chromatography Summary:Aqueous normal phase diamond hydride
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Chromatography Type:Normal phase

Analysis:

Analysis ID:AN001547
Analysis Type:MS
Instrument Name:Agilent 6220 TOF
Chromatography ID:CH001086
Num Factors:6
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST000943_AN001547_Results.txt
Units:peak counts
  
Analysis ID:AN001548
Analysis Type:MS
Instrument Name:Agilent 6220 TOF
Chromatography ID:CH001086
Num Factors:6
Has Mz:1
Has Rt:1
Rt Units:Minutes
Results File:ST000943_AN001548_Results.txt
Units:peak counts
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