Summary of Study ST000944

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000649. The data can be accessed directly via it's Project DOI: 10.21228/M8SD52 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000944
Study TitleAmino Acids, Acylcarnitine, & Insulin for P20 Participants
Study TypeMS analysis
Study SummarySee attached document.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2018-03-09
Raw Data AvailableYes
Raw Data File Type(s)d
Chear StudyNo
Analysis Type DetailGC-MS/LC-MS
Release Date2020-09-12
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8SD52
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000649
Project DOI:doi: 10.21228/M8SD52
Project Title:Metabolomic profiles and development of metabolic risk during puberty in the ELEMENT cohort
Project Type:MS analysis
Project Summary:Prospective investigation of amino acids and acylcarnitine metabolites with change in metabolic biomarkers during the pubertal transition among 208 peripubertal youth.
Institute:University of Michigan
Department:Nutritional Sciences
Laboratory:Peterson Lab
Last Name:Perng
First Name:Wei
Address:Ann Arbor, MI
Email:perngwei@umich.edu
Phone:734-764-7594

Subject:

Subject ID:SU000983
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id SampleType
SA055979S00027034Blinded
SA055980S00027033Blinded
SA055981S00027035Blinded
SA055982S00027036Blinded
SA055983S00027037Blinded
SA055984S00027032Blinded
SA055985S00027031Blinded
SA055986S00027027Blinded
SA055987S00027028Blinded
SA055988S00027029Blinded
SA055989S00027030Blinded
SA055990S00027038Blinded
SA055991S00027040Blinded
SA055992S00027047Blinded
SA055993S00027046Blinded
SA055994S00027048Blinded
SA055995S00027049Blinded
SA055996S00027050Blinded
SA055997S00027045Blinded
SA055998S00027044Blinded
SA055999S00027026Blinded
SA056000S00027041Blinded
SA056001S00027042Blinded
SA056002S00027043Blinded
SA056003S00027039Blinded
SA056004S00027024Blinded
SA056005S00027008Blinded
SA056006S00027007Blinded
SA056007S00027009Blinded
SA056008S00027010Blinded
SA056009S00027011Blinded
SA056010S00027006Blinded
SA056011S00027005Blinded
SA056012S00027001Blinded
SA056013S00027002Blinded
SA056014S00027003Blinded
SA056015S00027004Blinded
SA056016S00027012Blinded
SA056017S00027013Blinded
SA056018S00027021Blinded
SA056019S00027020Blinded
SA056020S00027022Blinded
SA056021S00027023Blinded
SA056022S00027051Blinded
SA056023S00027019Blinded
SA056024S00027018Blinded
SA056025S00027014Blinded
SA056026S00027015Blinded
SA056027S00027016Blinded
SA056028S00027017Blinded
SA056029S00027025Blinded
SA056030S00027053Blinded
SA056031S00027087Blinded
SA056032S00027086Blinded
SA056033S00027088Blinded
SA056034S00027089Blinded
SA056035S00027090Blinded
SA056036S00027085Blinded
SA056037S00027084Blinded
SA056038S00027080Blinded
SA056039S00027081Blinded
SA056040S00027082Blinded
SA056041S00027083Blinded
SA056042S00027091Blinded
SA056043S00027092Blinded
SA056044S00027100Blinded
SA056045S00027099Blinded
SA056046S00027101Blinded
SA056047S00027102Blinded
SA056048S00027064Blinded
SA056049S00027098Blinded
SA056050S00027097Blinded
SA056051S00027093Blinded
SA056052S00027094Blinded
SA056053S00027095Blinded
SA056054S00027096Blinded
SA056055S00027079Blinded
SA056056S00027078Blinded
SA056057S00027060Blinded
SA056058S00027059Blinded
SA056059S00027061Blinded
SA056060S00027062Blinded
SA056061S00027063Blinded
SA056062S00027058Blinded
SA056063S00027057Blinded
SA056064S00027000Blinded
SA056065S00027054Blinded
SA056066S00027055Blinded
SA056067S00027056Blinded
SA056068S00027065Blinded
SA056069S00027066Blinded
SA056070S00027074Blinded
SA056071S00027073Blinded
SA056072S00027075Blinded
SA056073S00027076Blinded
SA056074S00027077Blinded
SA056075S00027072Blinded
SA056076S00027071Blinded
SA056077S00027067Blinded
SA056078S00027068Blinded
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Collection:

Collection ID:CO000977
Collection Summary:None
Sample Type:Plasma;Feces;Cells;Tissue

Treatment:

Treatment ID:TR000997
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000990
Sampleprep Summary:Amino acids are analyzed using the Phenomenex EZfaast kit. Samples are extracted, semi-purified, derivitized and measured by EI-GCMS using norvaline as an internal standard for normalization. See reference for User Guide and analytical details. Analytes are reported as uM with CV's generally 10%, but Histidine is exceptional with a CV ~30
Sampleprep Protocol Filename:Amino_acids_analysis.pdf

Chromatography:

Chromatography ID:CH001087
Chromatography Summary:None
Methods Filename:EZGCMS_1.M
Instrument Name:Agilent 7890N
Column Name:Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm (Phenomenex)
Chromatography Type:GC
  
Chromatography ID:CH001088
Methods Filename:QM-004-HSS_50mm_ACar+_MRM-Insert-gradtest3
Instrument Name:Agilent 6490A
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN001549
Analysis Type:MS
Instrument Name:Agilent 7890N
Chromatography ID:CH001087
Num Factors:1
Num Metabolites:22
Units:uM
  
Analysis ID:AN001550
Analysis Type:MS
Instrument Name:Agilent 6490A QQQ
Chromatography ID:CH001088
Num Factors:1
Num Metabolites:27
Units:uM
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