Summary of Study ST000945

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000650. The data can be accessed directly via it's Project DOI: 10.21228/M8NM34 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000945
Study TitleThe gut microbiota influence the basal physiological parameters in equine athletes
Study SummaryComparison of serum metabolome and feces metagenome data from 52 horses before and after an endurance competition.
Institute
INRA
Last NameMach
First NameNúria
AddressDomaine de Vilvert, 78352 Jouy en Josas, France
Emailnuria.mach@inra.fr
Phone+33 (0)1 34 65 26 75
Submit Date2018-03-15
Analysis Type DetailNMR
Release Date2019-07-17
Release Version1
Núria Mach Núria Mach
https://dx.doi.org/10.21228/M8NM34
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000650
Project DOI:doi: 10.21228/M8NM34
Project Title:The gut microbiota influence the basal physiological parameters in equine athletes
Project Type:Comparison metabolome time
Project Summary:Comparison of serum metabolome and feces metagenome data from 52 horses before and after an endurance competition.
Institute:INRA
Last Name:Mach
First Name:Núria
Address:Domaine Vilvert, 78352 Jouy en Josas, France
Email:nuria.mach@inra.fr
Phone:+33 (0)1 34 65 26 75

Subject:

Subject ID:SU000984
Subject Type:Mammal
Subject Species:Equus caballus
Taxonomy ID:9796

Factors:

Subject type: Mammal; Subject species: Equus caballus (Factor headings shown in green)

mb_sample_id local_sample_id Time
SA056187ECAOMIC.237_T0T0
SA056188ECAOMIC.236_T0T0
SA056189ECAOMIC.235_T0T0
SA056190ECAOMIC.238_T0T0
SA056191ECAOMIC.239_T0T0
SA056192ECAOMIC.242_T0T0
SA056193ECAOMIC.241_T0T0
SA056194ECAOMIC.234_T0T0
SA056195ECAOMIC.232_T0T0
SA056196ECAOMIC.227_T0T0
SA056197ECAOMIC.226_T0T0
SA056198ECAOMIC.224_T0T0
SA056199ECAOMIC.228_T0T0
SA056200ECAOMIC.229_T0T0
SA056201ECAOMIC.243_T0T0
SA056202ECAOMIC.231_T0T0
SA056203ECAOMIC.201_T0T0
SA056204ECAOMIC.244_T0T0
SA056205ECAOMIC.256_T0T0
SA056206ECAOMIC.255_T0T0
SA056207ECAOMIC.254_T0T0
SA056208ECAOMIC.257_T0T0
SA056209ECAOMIC.259_T0T0
SA056210ECAOMIC.262_T0T0
SA056211ECAOMIC.260_T0T0
SA056212ECAOMIC.253_T0T0
SA056213ECAOMIC.251_T0T0
SA056214ECAOMIC.246_T0T0
SA056215ECAOMIC.245_T0T0
SA056216ECAOMIC.247_T0T0
SA056217ECAOMIC.248_T0T0
SA056218ECAOMIC.250_T0T0
SA056219ECAOMIC.249_T0T0
SA056220ECAOMIC.222_T0T0
SA056221ECAOMIC.233_T0T0
SA056222ECAOMIC.213_T0T0
SA056223ECAOMIC.211_T0T0
SA056224ECAOMIC.214_T0T0
SA056225ECAOMIC.215_T0T0
SA056226ECAOMIC.216_T0T0
SA056227ECAOMIC.210_T0T0
SA056228ECAOMIC.209_T0T0
SA056229ECAOMIC.203_T0T0
SA056230ECAOMIC.202_T0T0
SA056231ECAOMIC.204_T0T0
SA056232ECAOMIC.205_T0T0
SA056233ECAOMIC.206_T0T0
SA056234ECAOMIC.217_T0T0
SA056235ECAOMIC.212_T0T0
SA056236ECAOMIC.218_T0T0
SA056237ECAOMIC.220_T0T0
SA056238ECAOMIC.221_T0T0
SA056239ECAOMIC.205_T1T1
SA056240ECAOMIC.251_T1T1
SA056241ECAOMIC.247_T1T1
SA056242ECAOMIC.253_T1T1
SA056243ECAOMIC.220_T1T1
SA056244ECAOMIC.226_T1T1
SA056245ECAOMIC.250_T1T1
SA056246ECAOMIC.209_T1T1
SA056247ECAOMIC.249_T1T1
SA056248ECAOMIC.206_T1T1
SA056249ECAOMIC.254_T1T1
SA056250ECAOMIC.248_T1T1
SA056251ECAOMIC.255_T1T1
SA056252ECAOMIC.260_T1T1
SA056253ECAOMIC.202_T1T1
SA056254ECAOMIC.222_T1T1
SA056255ECAOMIC.262_T1T1
SA056256ECAOMIC.201_T1T1
SA056257ECAOMIC.259_T1T1
SA056258ECAOMIC.257_T1T1
SA056259ECAOMIC.227_T1T1
SA056260ECAOMIC.224_T1T1
SA056261ECAOMIC.256_T1T1
SA056262ECAOMIC.203_T1T1
SA056263ECAOMIC.204_T1T1
SA056264ECAOMIC.246_T1T1
SA056265ECAOMIC.236_T1T1
SA056266ECAOMIC.229_T1T1
SA056267ECAOMIC.214_T1T1
SA056268ECAOMIC.237_T1T1
SA056269ECAOMIC.238_T1T1
SA056270ECAOMIC.235_T1T1
SA056271ECAOMIC.215_T1T1
SA056272ECAOMIC.216_T1T1
SA056273ECAOMIC.232_T1T1
SA056274ECAOMIC.233_T1T1
SA056275ECAOMIC.217_T1T1
SA056276ECAOMIC.234_T1T1
SA056277ECAOMIC.213_T1T1
SA056278ECAOMIC.239_T1T1
SA056279ECAOMIC.244_T1T1
SA056280ECAOMIC.211_T1T1
SA056281ECAOMIC.218_T1T1
SA056282ECAOMIC.245_T1T1
SA056283ECAOMIC.210_T1T1
SA056284ECAOMIC.243_T1T1
SA056285ECAOMIC.221_T1T1
SA056286ECAOMIC.228_T1T1
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Collection:

Collection ID:CO000978
Collection Summary:Blood samples for metabolome pofiling were obtained from the jugular vein at rest (Basal, T0) and/or immediately after the end of the competition (T1). Pretreatment of the blood samples was carried out immediately after the collection because the access to refrigeration and electrical power supply was available under the field conditions. Briefly, whole blood samples from each horse were collected in sodium fluoride and oxalate tubes for metabolome profiling in order to inhibit further glycolysis that may increase the lactate levels after sampling. Whole blood draw for plasma generation was put at once at 4ºC to minimize the metabolic activity of cells and enzymes and kept the metabolite pattern almost stable. Clotting time at 4ºC was strictly controlled for all samples to avoid cell lyses and affect the components of the metabolome. After clotting at 4ºC, the plasma was separated from the blood cells, subsequently transported to the lab at 4ºC and frozen at -80 °C (no more than 5 h later, in all cases).
Sample Type:Blood (whole)

Treatment:

Treatment ID:TR000998
Treatment Summary:Two time course comparison of metabolome: T1 (post-endurance competition) relative to T0 (baseline, pre- endurance competition).

Sample Preparation:

Sampleprep ID:SP000991
Sampleprep Summary:The plasmas were thawed at room temperature. In the 5 mm NMR tubes, 600 µL of plasma was added with 100 µL deuterium oxide for field locking.
Sampleprep Protocol Filename:MO-Blood-Horse.docx

Analysis:

Analysis ID:AN001551
Analysis Type:NMR
Num Factors:2

NMR:

NMR ID:NM000121
Analysis ID:AN001551
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:500 MHz
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