Summary of Study ST000949

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000653. The data can be accessed directly via it's Project DOI: 10.21228/M88D75 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000949
Study TitleHuman NK vs T cell metabolism using 13C-Glucose tracer (part I)
Study Typeisotope tracer
Study SummaryNK versus CD8 T naïve and CD8 T memory SIRM experiment using 13C-Glucose tracer
Institute
University of Kentucky
DepartmentMarkey Cancer Center
Last NamePresnell
First NameSteve
AddressDept Of Pathology, UKMC
Emailsrpres2@uky.edu
Phone000-000-0000
Submit Date2018-04-13
Study CommentsFor additional comments about the study, like listing a publication.
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2019-05-15
Release Version1
Steve Presnell Steve Presnell
https://dx.doi.org/10.21228/M88D75
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000653
Project DOI:doi: 10.21228/M88D75
Project Title:NK versus CD8 T naïve and CD8 T memory
Project Summary:Exp to look at lactate/ alanine excreted in supernatant, lactate, citrulline and other TCA intermediates inside cells
Institute:University of Kentucky
Department:Markey Cancer Center
Last Name:Lutz
First Name:Charlie
Address:Dept Of Pathology, UKMC
Email:ctlutz2@uky.edu
Phone:000-000-0000

Subject:

Subject ID:SU000988
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id cell_type
SA05709220150505_T_memory_plate5-supCD8_T_memory
SA05709320150505_T_memory_plate6-supCD8_T_memory
SA05709420150505_T_memory_plate5-cellsCD8_T_memory
SA05709520150505_T_memory_plate6-cellsCD8_T_memory
SA05709620150505_T_naive_plate4-cellsCD8_T_naive
SA05709720150505_T_naive_plate4-supCD8_T_naive
SA05709820150505_T_naive_plate3-supCD8_T_naive
SA05709920150505_T_naive_plate3-cellsCD8_T_naive
SA05710020150505_NK_plate1-supNK
SA05710120150505_NK_plate1-cellsNK
SA05710220150505_NK_plate2-cellsNK
SA05710320150505_NK_plate2-supNK
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000982
Collection Summary:Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate.
Collection Protocol ID:blood_collection cell_quench_collection cells_media
Collection Protocol Filename:WS_Fan_Quench_cell_tissue.pdf
Sample Type:T-Cells

Treatment:

Treatment ID:TR001002
Treatment Summary:Grown in unlabeled glucose. Grown in labeled glucose. Normal human, no treatment.
Treatment Protocol ID:12CGlc 13CGlc normal

Sample Preparation:

Sampleprep ID:SP000995
Sampleprep Summary:Cells of interest extracted from the blood. Polar extraction from cells.
Sampleprep Protocol ID:cell_extraction polar_extraction
Sampleprep Protocol Filename:WS_Fan_Extract_Polar_Lipid_Prot.pdf

Chromatography:

Chromatography ID:CH001092
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

Analysis:

Analysis ID:AN001558
Analysis Type:MS
Instrument Name:GC-EI-MS, Thermo ITQ
Chromatography ID:CH001092
Num Factors:3
Num Metabolites:244
Units:normalized peak area
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