Summary of Study ST000950

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000653. The data can be accessed directly via it's Project DOI: 10.21228/M88D75 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000950
Study TitleHuman NK vs T cell metabolism using 13C-Glucose tracer with/out galactose (part II)
Study Typeisotope tracer
Study SummaryNK versus CD8 T naïve and CD8 T memory SIRM experiment using 13C-Glucose tracer with/out galactose
Institute
University of Kentucky
DepartmentMarkey Cancer Center
Last NamePresnell
First NameSteve
AddressDept Of Pathology, UKMC
Emailsrpres2@uky.edu
Phone000-000-0000
Submit Date2018-04-13
Study CommentsFor additional comments about the study, like listing a publication.
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2019-05-15
Release Version1
Steve Presnell Steve Presnell
https://dx.doi.org/10.21228/M88D75
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000653
Project DOI:doi: 10.21228/M88D75
Project Title:NK versus CD8 T naïve and CD8 T memory
Project Summary:Exp to look at lactate/ alanine excreted in supernatant, lactate, citrulline and other TCA intermediates inside cells
Institute:University of Kentucky
Department:Markey Cancer Center
Last Name:Lutz
First Name:Charlie
Address:Dept Of Pathology, UKMC
Email:ctlutz2@uky.edu
Phone:000-000-0000

Subject:

Subject ID:SU000989
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id cell_type
SA05710420150602_T_memory_plate11-cellsCD8_T_memory
SA05710520150602_T_memory_plate9-cellsCD8_T_memory
SA05710620150602_T_memory_plate10-cellsCD8_T_memory
SA05710720150602_T_memory_plate12-cellsCD8_T_memory
SA05710820150602_T_naive_plate7-cellsCD8_T_naive
SA05710920150602_T_naive_plate8-cellsCD8_T_naive
SA05711020150602_T_naive_plate6-cellsCD8_T_naive
SA05711120150602_T_naive_plate5-cellsCD8_T_naive
SA05711220150602_NK_plate2-cellsNK
SA05711320150602_NK_plate1-cellsNK
SA05711420150602_NK_plate3-cellsNK
SA05711520150602_NK_plate4-cellsNK
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000983
Collection Summary:Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate.
Collection Protocol ID:blood_collection cell_quench_collection cells_media
Collection Protocol Filename:WS_Fan_Quench_cell_tissue.pdf
Sample Type:T-Cells

Treatment:

Treatment ID:TR001003
Treatment Summary:Grown in unlabeled glucose and treated with galactose. Grown in labeled glucose and treated with galactose. Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose.
Treatment Protocol ID:galactose_12CGlc galactose_13CGlc normal normal_12CGlc normal_13CGlc

Sample Preparation:

Sampleprep ID:SP000996
Sampleprep Summary:Cells of interest extracted from the blood. Polar extraction from cells.
Sampleprep Protocol ID:cell_extraction polar_extraction
Sampleprep Protocol Filename:WS_Fan_Extract_Polar_Lipid_Prot.pdf

Chromatography:

Chromatography ID:CH001093
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

Analysis:

Analysis ID:AN001559
Analysis Type:MS
Instrument Name:GC-EI-MS, Thermo ITQ
Chromatography ID:CH001093
Num Factors:3
Num Metabolites:256
Units:normalized peak area
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