Summary of Study ST000951
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000653. The data can be accessed directly via it's Project DOI: 10.21228/M88D75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000951 |
| Study Title | Human NK vs T cell metabolism using 13C-Glucose tracer with/out oligomycin (part III) |
| Study Type | isotope tracer |
| Study Summary | NK versus CD8 T naïve and CD8 T memory SIRM experiment using 13C-Glucose tracer with/out oligomycin |
| Institute | University of Kentucky |
| Department | Markey Cancer Center |
| Last Name | Presnell |
| First Name | Steve |
| Address | Dept Of Pathology, UKMC |
| srpres2@uky.edu | |
| Phone | 000-000-0000 |
| Submit Date | 2018-04-13 |
| Study Comments | For additional comments about the study, like listing a publication. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-05-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000653 |
| Project DOI: | doi: 10.21228/M88D75 |
| Project Title: | NK versus CD8 T naïve and CD8 T memory |
| Project Summary: | Exp to look at lactate/ alanine excreted in supernatant, lactate, citrulline and other TCA intermediates inside cells |
| Institute: | University of Kentucky |
| Department: | Markey Cancer Center |
| Last Name: | Lutz |
| First Name: | Charlie |
| Address: | Dept Of Pathology, UKMC |
| Email: | ctlutz2@uky.edu |
| Phone: | 000-000-0000 |
Subject:
| Subject ID: | SU000990 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | cell_type |
|---|---|---|
| SA057116 | 20150818_T_memory_plate8 | CD8_T_memory |
| SA057117 | 20150818_T_memory_plate7 | CD8_T_memory |
| SA057118 | 20150818_T_memory_plate9 | CD8_T_memory |
| SA057119 | 20150818_T_memory_plate10 | CD8_T_memory |
| SA057120 | 20150818_T_naive_plate5 | CD8_T_naive |
| SA057121 | 20150818_T_naive_plate6 | CD8_T_naive |
| SA057122 | 20150818_T_naive_plate4 | CD8_T_naive |
| SA057123 | 20150818_T_naive_plate3 | CD8_T_naive |
| SA057124 | 20150818_NK_plate2 | NK |
| SA057125 | 20150818_NK_plate1 | NK |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000984 |
| Collection Summary: | Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate. |
| Collection Protocol ID: | blood_collection cell_quench_collection cells_media |
| Collection Protocol Filename: | WS_Fan_Quench_cell_tissue.pdf |
| Sample Type: | T-Cells |
Treatment:
| Treatment ID: | TR001004 |
| Treatment Summary: | Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose. Grown in unlabeled glucose and treated with oligomycin. Grown in labeled glucose and treated with oligomycin. |
| Treatment Protocol ID: | normal normal_12CGlc normal_13CGlc oligomycin_12CGlc oligomycin_13CGlc |
Sample Preparation:
| Sampleprep ID: | SP000997 |
| Sampleprep Summary: | Cells of interest extracted from the blood. Polar extraction from cells. |
| Sampleprep Protocol ID: | cell_extraction polar_extraction |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf |
Combined analysis:
| Analysis ID | AN001560 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | GC-EI-MS, Thermo ITQ |
| Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| MS Type | EI |
| MS instrument type | GC-ITQ |
| MS instrument name | Thermo ITQ |
| Ion Mode | POSITIVE |
| Units | normalized peak area |
Chromatography:
| Chromatography ID: | CH001094 |
| Chromatography Summary: | Targeted GC |
| Instrument Name: | GC-EI-MS, Thermo ITQ |
| Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001438 |
| Analysis ID: | AN001560 |
| Instrument Name: | Thermo ITQ |
| Instrument Type: | GC-ITQ |
| MS Type: | EI |
| MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
| Ion Mode: | POSITIVE |
