Summary of Study ST000952
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000653. The data can be accessed directly via it's Project DOI: 10.21228/M88D75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000952 |
| Study Title | Human NK vs T cell metabolism using 13C-Glucose tracer with/out oligomycin and galactose (part IV) |
| Study Type | isotope tracer |
| Study Summary | Human NK vs T cell metabolism using 13C-Glucose tracer with/out oligomycin and galactose |
| Institute | University of Kentucky |
| Department | Markey Cancer Center |
| Last Name | Presnell |
| First Name | Steve |
| Address | Dept Of Pathology, UKMC |
| srpres2@uky.edu | |
| Phone | 000-000-0000 |
| Submit Date | 2018-04-13 |
| Study Comments | For additional comments about the study, like listing a publication. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-05-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000653 |
| Project DOI: | doi: 10.21228/M88D75 |
| Project Title: | NK versus CD8 T naïve and CD8 T memory |
| Project Summary: | Exp to look at lactate/ alanine excreted in supernatant, lactate, citrulline and other TCA intermediates inside cells |
| Institute: | University of Kentucky |
| Department: | Markey Cancer Center |
| Last Name: | Lutz |
| First Name: | Charlie |
| Address: | Dept Of Pathology, UKMC |
| Email: | ctlutz2@uky.edu |
| Phone: | 000-000-0000 |
Subject:
| Subject ID: | SU000991 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | cell_type |
|---|---|---|
| SA057126 | 20150821_T_plate7 | CD8_T |
| SA057127 | 20150821_T_plate8 | CD8_T |
| SA057128 | 20150821_T_plate9 | CD8_T |
| SA057129 | 20150821_T_plate6 | CD8_T |
| SA057130 | 20150821_T_plate10 | CD8_T |
| SA057131 | 20150821_NK_plate2 | NK |
| SA057132 | 20150821_NK_plate3 | NK |
| SA057133 | 20150821_NK_plate4 | NK |
| SA057134 | 20150821_NK_plate5 | NK |
| SA057135 | 20150821_NK_plate1 | NK |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000985 |
| Collection Summary: | Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate. |
| Collection Protocol ID: | blood_collection cell_quench_collection cells_media |
| Collection Protocol Filename: | WS_Fan_Quench_cell_tissue.pdf |
| Sample Type: | T-Cells |
Treatment:
| Treatment ID: | TR001005 |
| Treatment Summary: | Grown in unlabeled glucose and treated with galactose. Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose. Grown in unlabeled glucose and treated with oligomycin. Grown in labeled glucose and treated with oligomycin. |
| Treatment Protocol ID: | galactose_12CGlc normal normal_12CGlc normal_13CGlc oligomycin_12CGlc oligomycin_13CGlc |
Sample Preparation:
| Sampleprep ID: | SP000998 |
| Sampleprep Summary: | Cells of interest extracted from the blood. Polar extraction from cells. |
| Sampleprep Protocol ID: | cell_extraction polar_extraction |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf |
Combined analysis:
| Analysis ID | AN001561 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | GC-EI-MS, Thermo ITQ |
| Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| MS Type | EI |
| MS instrument type | GC-ITQ |
| MS instrument name | Thermo ITQ |
| Ion Mode | POSITIVE |
| Units | normalized peak area |
Chromatography:
| Chromatography ID: | CH001095 |
| Chromatography Summary: | Targeted GC |
| Instrument Name: | GC-EI-MS, Thermo ITQ |
| Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001439 |
| Analysis ID: | AN001561 |
| Instrument Name: | Thermo ITQ |
| Instrument Type: | GC-ITQ |
| MS Type: | EI |
| MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
| Ion Mode: | POSITIVE |
