Summary of Study ST000952

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000653. The data can be accessed directly via it's Project DOI: 10.21228/M88D75 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000952
Study TitleHuman NK vs T cell metabolism using 13C-Glucose tracer with/out oligomycin and galactose (part IV)
Study Typeisotope tracer
Study SummaryHuman NK vs T cell metabolism using 13C-Glucose tracer with/out oligomycin and galactose
Institute
University of Kentucky
DepartmentMarkey Cancer Center
Last NamePresnell
First NameSteve
AddressDept Of Pathology, UKMC
Emailsrpres2@uky.edu
Phone000-000-0000
Submit Date2018-04-13
Study CommentsFor additional comments about the study, like listing a publication.
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2019-05-15
Release Version1
Steve Presnell Steve Presnell
https://dx.doi.org/10.21228/M88D75
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000653
Project DOI:doi: 10.21228/M88D75
Project Title:NK versus CD8 T naïve and CD8 T memory
Project Summary:Exp to look at lactate/ alanine excreted in supernatant, lactate, citrulline and other TCA intermediates inside cells
Institute:University of Kentucky
Department:Markey Cancer Center
Last Name:Lutz
First Name:Charlie
Address:Dept Of Pathology, UKMC
Email:ctlutz2@uky.edu
Phone:000-000-0000

Subject:

Subject ID:SU000991
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id cell_type
SA05712620150821_T_plate7CD8_T
SA05712720150821_T_plate8CD8_T
SA05712820150821_T_plate9CD8_T
SA05712920150821_T_plate6CD8_T
SA05713020150821_T_plate10CD8_T
SA05713120150821_NK_plate2NK
SA05713220150821_NK_plate3NK
SA05713320150821_NK_plate4NK
SA05713420150821_NK_plate5NK
SA05713520150821_NK_plate1NK
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000985
Collection Summary:Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate.
Collection Protocol ID:blood_collection cell_quench_collection cells_media
Collection Protocol Filename:WS_Fan_Quench_cell_tissue.pdf
Sample Type:T-Cells

Treatment:

Treatment ID:TR001005
Treatment Summary:Grown in unlabeled glucose and treated with galactose. Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose. Grown in unlabeled glucose and treated with oligomycin. Grown in labeled glucose and treated with oligomycin.
Treatment Protocol ID:galactose_12CGlc normal normal_12CGlc normal_13CGlc oligomycin_12CGlc oligomycin_13CGlc

Sample Preparation:

Sampleprep ID:SP000998
Sampleprep Summary:Cells of interest extracted from the blood. Polar extraction from cells.
Sampleprep Protocol ID:cell_extraction polar_extraction
Sampleprep Protocol Filename:WS_Fan_Extract_Polar_Lipid_Prot.pdf

Chromatography:

Chromatography ID:CH001095
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC

Analysis:

Analysis ID:AN001561
Analysis Type:MS
Instrument Name:GC-EI-MS, Thermo ITQ
Chromatography ID:CH001095
Num Factors:2
Num Metabolites:295
Units:normalized peak area
  logo