Summary of Study ST000953
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000654. The data can be accessed directly via it's Project DOI: 10.21228/M84M4V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000953 |
| Study Title | Identification of putative cryoprotectant metabolites in Aphaenogaster picea |
| Study Type | three treatments (control, cold shock, cold acclimation) |
| Study Summary | Quantification of metabolite abundances produced in response to acute or chronic cold stress (4 replicates for each of three treatments) |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Cahan |
| First Name | Sara |
| Address | Department of Biology, Univ. of VT, Burlington, VT 05405 |
| scahan@uvm.edu | |
| Phone | 8026562962 |
| Submit Date | 2018-04-11 |
| Num Groups | 3 |
| Total Subjects | 12 |
| Study Comments | SECIM pilot and feasibility, NIH U24 DK097209 (if applicable) |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-05-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000654 |
| Project DOI: | doi: 10.21228/M84M4V |
| Project Title: | The effect of a trehalose diet on cold tolerance in Apheanogaster picea |
| Project Summary: | Investigation of physiological resistance to low temperatures in a northern freeze-avoidant ant species. |
| Institute: | University of Vermont |
| Department: | Biology |
| Last Name: | Cahan |
| First Name: | Sara |
| Address: | Department of Biology, Univ. of VT, Burlington, VT 05405 |
| Email: | scahan@uvm.edu |
| Phone: | (802)656-2962 |
Subject:
| Subject ID: | SU000992 |
| Subject Type: | Insect |
| Subject Species: | Aphaenogaster picea |
| Taxonomy ID: | 602698 |
| Genotype Strain: | Field colonies of forest ant |
Factors:
Subject type: Insect; Subject species: Aphaenogaster picea (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA057136 | EW-06-ACC | ACC |
| SA057137 | EW-02-ACC | ACC |
| SA057138 | EW-10-ACC | ACC |
| SA057139 | EW-07-ACC | ACC |
| SA057140 | EW-07-RCH | RCH |
| SA057141 | EW-06-RCH | RCH |
| SA057142 | EW-02-RCH | RCH |
| SA057143 | EW-10-RCH | RCH |
| SA057144 | EW-07-RT | RT |
| SA057145 | EW-06-RT | RT |
| SA057146 | EW-02-RT | RT |
| SA057147 | EW-10-RT | RT |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO000986 |
| Collection Summary: | Live animals flash-frozen in liquid nitrogen and kept at -80. Sample source is whole animal tissue (~40 workers/sample) |
| Sample Type: | Whole Tissue |
Treatment:
| Treatment ID: | TR001006 |
| Treatment Summary: | Control - at 25 degrees C. Cold shock - 0 degrees C for one hour, recovery at 25 degrees one hour. Acclimation - two weeks at 4 degrees C. Acronyms used in study design for treatment (ACC - acclimation, RCH - cold shock, RT - room temperature) |
Sample Preparation:
| Sampleprep ID: | SP000999 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | GMetabolomics_LCMS_Protocol_092117.pdf Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
| Sample Spiking: | Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
Combined analysis:
| Analysis ID | AN001562 | AN001563 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Height | peak height |
Chromatography:
| Chromatography ID: | CH001096 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 350 ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001440 |
| Analysis ID: | AN001562 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001441 |
| Analysis ID: | AN001563 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
