Summary of Study ST000953
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000654. The data can be accessed directly via it's Project DOI: 10.21228/M84M4V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000953 |
Study Title | Identification of putative cryoprotectant metabolites in Aphaenogaster picea |
Study Type | three treatments (control, cold shock, cold acclimation) |
Study Summary | Quantification of metabolite abundances produced in response to acute or chronic cold stress (4 replicates for each of three treatments) |
Institute | University of Florida |
Department | SECIM |
Last Name | Cahan |
First Name | Sara |
Address | Department of Biology, Univ. of VT, Burlington, VT 05405 |
scahan@uvm.edu | |
Phone | 8026562962 |
Submit Date | 2018-04-11 |
Num Groups | 3 |
Total Subjects | 12 |
Study Comments | SECIM pilot and feasibility, NIH U24 DK097209 (if applicable) |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2019-05-15 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000654 |
Project DOI: | doi: 10.21228/M84M4V |
Project Title: | The effect of a trehalose diet on cold tolerance in Apheanogaster picea |
Project Summary: | Investigation of physiological resistance to low temperatures in a northern freeze-avoidant ant species. |
Institute: | University of Vermont |
Department: | Biology |
Last Name: | Cahan |
First Name: | Sara |
Address: | Department of Biology, Univ. of VT, Burlington, VT 05405 |
Email: | scahan@uvm.edu |
Phone: | (802)656-2962 |
Subject:
Subject ID: | SU000992 |
Subject Type: | Insect |
Subject Species: | Aphaenogaster picea |
Taxonomy ID: | 602698 |
Genotype Strain: | Field colonies of forest ant |
Species Group: | Insects |
Factors:
Subject type: Insect; Subject species: Aphaenogaster picea (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment |
---|---|---|
SA057136 | EW-06-ACC | ACC |
SA057137 | EW-02-ACC | ACC |
SA057138 | EW-10-ACC | ACC |
SA057139 | EW-07-ACC | ACC |
SA057140 | EW-07-RCH | RCH |
SA057141 | EW-06-RCH | RCH |
SA057142 | EW-02-RCH | RCH |
SA057143 | EW-10-RCH | RCH |
SA057144 | EW-07-RT | RT |
SA057145 | EW-06-RT | RT |
SA057146 | EW-02-RT | RT |
SA057147 | EW-10-RT | RT |
Showing results 1 to 12 of 12 |
Collection:
Collection ID: | CO000986 |
Collection Summary: | Live animals flash-frozen in liquid nitrogen and kept at -80. Sample source is whole animal tissue (~40 workers/sample) |
Sample Type: | Whole Tissue |
Treatment:
Treatment ID: | TR001006 |
Treatment Summary: | Control - at 25 degrees C. Cold shock - 0 degrees C for one hour, recovery at 25 degrees one hour. Acclimation - two weeks at 4 degrees C. Acronyms used in study design for treatment (ACC - acclimation, RCH - cold shock, RT - room temperature) |
Sample Preparation:
Sampleprep ID: | SP000999 |
Sampleprep Summary: | none |
Sampleprep Protocol Filename: | GMetabolomics_LCMS_Protocol_092117.pdf Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
Sample Spiking: | Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
Combined analysis:
Analysis ID | AN001562 | AN001563 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Height | peak height |
Chromatography:
Chromatography ID: | CH001096 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 350 ul/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001440 |
Analysis ID: | AN001562 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS001441 |
Analysis ID: | AN001563 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |