Summary of Study ST000957

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000658. The data can be accessed directly via it's Project DOI: 10.21228/M8MM5K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000957
Study TitleGlobal metabolomics of human milk fractions
Study Typecross sectional
Study SummaryPediatric obesity has more than doubled in children and tripled in adolescents over the past 30 years. Recent findings demonstrate that differences in energy harvesting bacteria promote obesity in the host and appear to be influenced by early life factors such as mode of delivery, maternal obesity, and breastfeeding. The goal of this proposal is to investigate how human milk impacts the infant gut microbiome during the first 12-months of life and identify the microbe-host interactions that mediates the protective role of breastfeeding on infant adiposity. The results of this exploratory study will characterize factors that influence microbial transmission between mothers and offspring and identify human milk compounds that stabilize a healthy infant microbiome with potential to reduce pediatric obesity.
Institute
University of Florida
DepartmentSECIM
LaboratoryR1-187
Last NameCarney
First NameOlivia
AddressClinical and Translational Research Building, University of Florida College of Medicine, 2004 Mowry Road, Gainesville, FL 32608
Emailocarney1@ufl.edu
Phone352-294-8361
Submit Date2018-04-15
Num Groups3
Total Subjects12
Study Comments12 samples (4 mothers with 3 human milk fractions: fat, skim and whole)
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Olivia Carney Olivia Carney
https://dx.doi.org/10.21228/M8MM5K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000658
Project DOI:doi: 10.21228/M8MM5K
Project Title:Human milk metabolomics and microb-host interactions associated with pediatric obesity
Project Type:observational longitudinal human study
Project Summary:Pediatric obesity has more than doubled in children and tripled in adolescents over the past 30 years. Recent findings demonstrate that differences in energy harvesting bacteria promote obesity in the host and appear to be influenced by early life factors such as mode of delivery, maternal obesity, and breastfeeding. The goal of this proposal is to investigate how human milk impacts the infant gut microbiome during the first 12-months of life and identify the microbe-host interactions that mediates the protective role of breastfeeding on infant adiposity. The results of this exploratory study will characterize factors that influence microbial transmission between mothers and offspring and identify human milk compounds that stabilize a healthy infant microbiome with potential to reduce pediatric obesity.
Institute:University of Florida
Department:Health Outcomes and Policy
Laboratory:R1-187
Last Name:Lemas
First Name:Dominick
Address:Clinical and Translational Research Building, University of Florida College of Medicine, 2004 Mowry Road, Rm 2234, Gainesville, FL 32608
Email:djlemas@ufl.edu
Phone:352-294-5971
Funding Source:Start-up Funds

Subject:

Subject ID:SU000996
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Milk fraction
SA057291BLS010Afat
SA057292BLS001Afat
SA057293BLS003Afat
SA057294BLS002Afat
SA057295BLS002A_2skim
SA057296BLS001A_2skim
SA057297BLS003A_2skim
SA057298BLS010A_2skim
SA057299BLS010A_3whole
SA057300BLS003A_3whole
SA057301BLS002A_3whole
SA057302BLS001A_3whole
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000990
Collection Summary:mothers will arrive in a fasted state in the morning and complete an infant feeding questionnaire as described previously (Lemas et al. 2016). Mothers will then pump ~30mL human milk (HM) into polypropylene vials and stored at -80ºC.
Sample Type:Human Milk

Treatment:

Treatment ID:TR001010
Treatment Summary:human milk samples were fractionated (fat and skim) using centrifugation and whole milk samples wer snap frozen at -80c.

Sample Preparation:

Sampleprep ID:SP001003
Sampleprep Summary:None
Sampleprep Protocol Filename:GMetabolomics_LCMS_Protocol_092117.pdf
Appendix_A_Internal_Standard_Prep_GLCMS.pdf

Combined analysis:

Analysis ID AN001570 AN001571
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units peak height peak height

Chromatography:

Chromatography ID:CH001100
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:350 ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001448
Analysis ID:AN001570
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001449
Analysis ID:AN001571
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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