Summary of Study ST000982
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000671. The data can be accessed directly via it's Project DOI: 10.21228/M8XX0K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000982 |
| Study Title | Analysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephala. |
| Study Summary | Analysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephala by HPLC-MS/MS. |
| Institute | Huazhong Agricultural University |
| Last Name | Xu |
| First Name | Jia |
| Address | Lion rock street, hung shan district, Wuhan, HuBei, 430070, China |
| xujia2018hzau@163.com | |
| Phone | 13018097215 |
| Submit Date | 2018-06-25 |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-08-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000671 |
| Project DOI: | doi: 10.21228/M8XX0K |
| Project Title: | Analysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephala. |
| Project Summary: | Analysis the concentration of betaine, choline, TMA, TMAO and amino acids in plasma in Megalobrama amblycephalab by HPLC-MS/MS |
| Institute: | Huazhong Agricultural University |
| Last Name: | Xu |
| First Name: | Jia |
| Address: | Lion rock street, hung shan district, Wuhan, HuBei, 430070, China |
| Email: | xujia2018hzau@163.com |
| Phone: | 13018097215 |
Subject:
| Subject ID: | SU001021 |
| Subject Type: | Fish |
| Subject Species: | Megalobrama amblycephala |
| Taxonomy ID: | 75352 |
Factors:
Subject type: Fish; Subject species: Megalobrama amblycephala (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet |
|---|---|---|
| SA060036 | 15 | Betaine supplementary for 16 weeks |
| SA060037 | 16 | Betaine supplementary for 16 weeks |
| SA060038 | 18 | Betaine supplementary for 16 weeks |
| SA060039 | 14 | Betaine supplementary for 16 weeks |
| SA060040 | 17 | Betaine supplementary for 16 weeks |
| SA060041 | 13 | Betaine supplementary for 16 weeks |
| SA060042 | 20 | Betaine supplementary for 4 weeks |
| SA060043 | 19 | Betaine supplementary for 4 weeks |
| SA060044 | 21 | Betaine supplementary for 4 weeks |
| SA060045 | 22 | Betaine supplementary for 4 weeks |
| SA060046 | 23 | Betaine supplementary for 4 weeks |
| SA060047 | 24 | Betaine supplementary for 4 weeks |
| SA060048 | 3 | Control group |
| SA060049 | 2 | Control group |
| SA060050 | 4 | Control group |
| SA060051 | 5 | Control group |
| SA060052 | 6 | Control group |
| SA060053 | 1 | Control group |
| SA060054 | 10 | High carbohydrates group |
| SA060055 | 9 | High carbohydrates group |
| SA060056 | 11 | High carbohydrates group |
| SA060057 | 12 | High carbohydrates group |
| SA060058 | 8 | High carbohydrates group |
| SA060059 | 7 | High carbohydrates group |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO001015 |
| Collection Summary: | After 16 weeks of the feeding trial, approximately 24h after the last feeding, fish in all tanks were anaesthetized with MS-222 (Sigma Aldrich, USA) at the concentration of 100 mg L-1. Fifteen specimens from each tank were selected randomly to obtain the blood. Blood was obtained from the caudal vein using sterile syringes with pre-added anticoagulant solution and then centrifuged (3000×g) at 4°C for 10 min to obtain the serum, which was quickly frozen in liquid nitrogen and stored at -80°C for biochemical assays. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR001035 |
| Treatment Summary: | Control diet (CD group) was formulated to contain 27.11±0.38% carbohydrates. The high-carbohydrate diet (HCD group) was formulated to contain over 36.75±0.92% of easily digestible carbohydrates (mostly from flour). The betaine treatment diet (HCB) to contain a high level of carbohydrates (35.64±0.43%) with 1% betaine supplemented for 16 weeks. HC4B group was fed a combination of HCD (first twelve weeks) and HCB diets (last four weeks). |
| Treatment Protocol Filename: | Experiment_methods.docx |
Sample Preparation:
| Sampleprep ID: | SP001028 |
| Sampleprep Summary: | Fifty μl of protein precipitant (containing NVL) was added to 50 μl of serum solution of each sample, mixed thoroughly, and centrifuged at 13200 rpm for 4 min. The supernatant (10 μl) was mixed with 50 μl methanol borate buffer (0.1 M, pH=8.8) thoroughly. For amino acids, the above solution was mixed with 20 μl of diethyl ethoxymethylene-malonate at 55°C for 15 min. |
Combined analysis:
| Analysis ID | AN001608 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Unspecified |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex API 3200 QTrap |
| Ion Mode | POSITIVE |
| Units | umol/L or ng/mL |
Chromatography:
| Chromatography ID: | CH001130 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Unspecified |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001486 |
| Analysis ID: | AN001608 |
| Instrument Name: | ABI Sciex API 3200 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
