Summary of Study ST001019

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000682. The data can be accessed directly via it's Project DOI: 10.21228/M8HQ2J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001019
Study Title	Lipidomic profiling of heart and plasma of mice following swim training versus pressure overload
Study Summary	Lipid profiling was performed on hearts and plasma from mice subjected to a physiological stimulus (4 weeks of swim exercise training) or pathological stimulus (4 weeks of pressure overload – transverse aortic constriction; TAC)
Institute	Baker Heart and Diabetes Institute
Laboratory	Cardiac Hypertrophy
Last Name	Tham
First Name	Yow Keat
Address	75 Commercial Rd, Melbourne 3004
Email	yowkeat.tham@baker.edu.au
Phone	+65385321266
Submit Date	2018-07-15
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2018-07-25
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000682
Project DOI:	doi: 10.21228/M8HQ2J
Project Title:	Lipidomic profiles of the heart and circulation in response to exercise versus cardiac pathology: A resource of potential biomarkers and drug targets
Project Summary:	Lipid profiling was performed on hearts and plasma from mice subjected to a physiological stimulus (4 weeks of swim exercise training) or pathological stimulus (4 weeks of pressure overload – transverse aortic constriction; TAC)
Institute:	Baker Heart and Diabetes Institute
Laboratory:	Cardiac Hypertrophy
Last Name:	Tham
First Name:	Yow Keat
Address:	75 Commercial Road, Melbourne 3004
Email:	yowkeat.tham@baker.edu.au
Phone:	+65385321266
Funding Source:	National Health and Medical Research Council

Subject:

Subject ID:	SU001058
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	FVB/N
Age Or Age Range:	16.5 to 18.5 weeks old
Weight Or Weight Range:	25 to 30 grams
Gender:	Male
Animal Animal Supplier:	in house breeding
Animal Feed:	Standard chow

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Tissue	Intervention
SA063942	#B10_0024 YKT_11/8/16_#15	Heart	NON SWIM
SA063943	#B10_0014 YKT_11/8/16_#5	Heart	NON SWIM
SA063944	#B10_0026 YKT_11/8/16_#17	Heart	NON SWIM
SA063945	#B10_0027 YKT_11/8/16_#18	Heart	NON SWIM
SA063946	#B10_0016 YKT_11/8/16_#7	Heart	NON SWIM
SA063947	#B10_0023 YKT_11/8/16_#14	Heart	NON SWIM
SA063948	#B10_0051 YKT_11/8/16_#41	Heart	SHAM
SA063949	#B10_0043 YKT_11/8/16_#33	Heart	SHAM
SA063950	#B10_0036 YKT_11/8/16_#26	Heart	SHAM
SA063951	#B10_0050 YKT_11/8/16_#40	Heart	SHAM
SA063952	#B10_0030 YKT_11/8/16_#21	Heart	SHAM
SA063953	#B10_0025 YKT_11/8/16_#16	Heart	SWIM
SA063954	#B10_0017 YKT_11/8/16_#8	Heart	SWIM
SA063955	#B10_0015 YKT_11/8/16_#6	Heart	SWIM
SA063956	#B10_0018 YKT_11/8/16_#9	Heart	SWIM
SA063957	#B10_0019 YKT_11/8/16_#10	Heart	SWIM
SA063958	#B10_0022 YKT_11/8/16_#13	Heart	SWIM
SA063959	#B10_0040 YKT_11/8/16_#30	Heart	TACMOD
SA063960	#B10_0035 YKT_11/8/16_#25	Heart	TACMOD
SA063961	#B10_0033 YKT_11/8/16_#24	Heart	TACMOD
SA063962	#B10_0032 YKT_11/8/16_#23	Heart	TACMOD
SA063963	#B10_0039 YKT_11/8/16_#29	Heart	TACMOD
SA063964	#B10_0031 YKT_11/8/16_#22	Heart	TACMOD
SA063965	#B10_0041 YKT_11/8/16_#31	Heart	TACSEV
SA063966	#B10_0049 YKT_11/8/16_#39	Heart	TACSEV
SA063967	#B10_0042 YKT_11/8/16_#32	Heart	TACSEV
SA063968	#B10_0048 YKT_11/8/16_#38	Heart	TACSEV
SA063969	#B10_0044 YKT_11/8/16_#34	Heart	TACSEV
SA063970	#B10_0047 YKT_11/8/16_#37	Heart	TACSEV
SA063971	#B10_0089 YKT_11/8/16_#77	Plasma	NON SWIM
SA063972	#B10_0091 YKT_11/8/16_#79	Plasma	NON SWIM
SA063973	#B10_0092 YKT_11/8/16_#80	Plasma	NON SWIM
SA063974	#B10_0088 YKT_11/8/16_#76	Plasma	NON SWIM
SA063975	#B10_0078 YKT_11/8/16_#67	Plasma	NON SWIM
SA063976	#B10_0081 YKT_11/8/16_#69	Plasma	NON SWIM
SA063977	#B10_0095 YKT_11/8/16_#83	Plasma	SHAM
SA063978	#B10_0108 YKT_11/8/16_#95	Plasma	SHAM
SA063979	#B10_0115 YKT_11/8/16_#102	Plasma	SHAM
SA063980	#B10_0116 YKT_11/8/16_#103	Plasma	SHAM
SA063981	#B10_0100 YKT_11/8/16_#88	Plasma	SHAM
SA063982	#B10_0087 YKT_11/8/16_#75	Plasma	SWIM
SA063983	#B10_0082 YKT_11/8/16_#70	Plasma	SWIM
SA063984	#B10_0080 YKT_11/8/16_#68	Plasma	SWIM
SA063985	#B10_0090 YKT_11/8/16_#78	Plasma	SWIM
SA063986	#B10_0083 YKT_11/8/16_#71	Plasma	SWIM
SA063987	#B10_0084 YKT_11/8/16_#72	Plasma	SWIM
SA063988	#B10_0105 YKT_11/8/16_#92	Plasma	TACMOD
SA063989	#B10_0104 YKT_11/8/16_#91	Plasma	TACMOD
SA063990	#B10_0096 YKT_11/8/16_#84	Plasma	TACMOD
SA063991	#B10_0097 YKT_11/8/16_#85	Plasma	TACMOD
SA063992	#B10_0098 YKT_11/8/16_#86	Plasma	TACMOD
SA063993	#B10_0099 YKT_11/8/16_#87	Plasma	TACMOD
SA063994	#B10_0114 YKT_11/8/16_#101	Plasma	TACSEV
SA063995	#B10_0106 YKT_11/8/16_#93	Plasma	TACSEV
SA063996	#B10_0107 YKT_11/8/16_#94	Plasma	TACSEV
SA063997	#B10_0109 YKT_11/8/16_#96	Plasma	TACSEV
SA063998	#B10_0112 YKT_11/8/16_#99	Plasma	TACSEV
SA063999	#B10_0113 YKT_11/8/16_#100	Plasma	TACSEV

Showing results 1 to 58 of 58

Showing results 1 to 58 of 58

Collection:

Collection ID:	CO001052
Collection Summary:	At study endpoint, prior to tissue collection, mice were anesthetized with sodium pentobarbitone (Lethabarb, 80mg/kg). Once mice had reached an adequate level of anesthesia (assessed by testing the pedal reflex), blood was collected via cardiac puncture using a 25 G needle and 1 ml syringe. This procedure was followed by cervical dislocation. Collected blood was stored on ice in K2EDTA tubes (Beckton Dickinson), centrifuged at 4°C, 4000 g for 10 min, and plasma collected into fresh tubes and stored at -80°C.
Sample Type:	Blood (plasma)
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR001072
Treatment Summary:	Swim training - Physiological hypertrophy in mice was achieved via swim training twice daily for 4 weeks, as previously described (McMullen et al., 2003). Swim training commenced in adult mice at approximately 3 months of age with 10 min sessions on the first day, with 10 min increments each day until the maximum of 90 min per session was reached. Mice were rested for at least 4 hours between each session, and water temperature was maintained between 30 to 32°C to avoid thermal stress. At the end of every session, mice were individually towel dried to prevent hypothermia. Plasma and hearts were collected 3 h after the last swim session. Transverse aortic constriction - Male mice were subjected to either sham or TAC surgery at approximately 3-3.5 months of age, as previously described (Xu et al., 2008). Two variations of TAC were performed, designated TAC-moderate (TAC-MOD) and TAC-severe (TAC-SEV). Aortic banding performed on the TAC-MOD mice used a blunt 25 G needle (outside diameter 0.5 mm) placed next to the aorta during ligation to obtain a consistent degree of banding (constriction of blood vessel from ~1 mm to 0.5 mm), while a 27 G needle (outside diameter 0.4 mm) was used for the TAC-SEV group (constriction of blood vessel from ~1 mm to 0.4 mm). Sham operated mice were subjected to the same procedure but no ligation was made.

Treatment ID:

TR001072

Treatment Summary:

Swim training - Physiological hypertrophy in mice was achieved via swim training twice daily for 4 weeks, as previously described (McMullen et al., 2003). Swim training commenced in adult mice at approximately 3 months of age with 10 min sessions on the first day, with 10 min increments each day until the maximum of 90 min per session was reached. Mice were rested for at least 4 hours between each session, and water temperature was maintained between 30 to 32°C to avoid thermal stress. At the end of every session, mice were individually towel dried to prevent hypothermia. Plasma and hearts were collected 3 h after the last swim session. Transverse aortic constriction - Male mice were subjected to either sham or TAC surgery at approximately 3-3.5 months of age, as previously described (Xu et al., 2008). Two variations of TAC were performed, designated TAC-moderate (TAC-MOD) and TAC-severe (TAC-SEV). Aortic banding performed on the TAC-MOD mice used a blunt 25 G needle (outside diameter 0.5 mm) placed next to the aorta during ligation to obtain a consistent degree of banding (constriction of blood vessel from ~1 mm to 0.5 mm), while a 27 G needle (outside diameter 0.4 mm) was used for the TAC-SEV group (constriction of blood vessel from ~1 mm to 0.4 mm). Sham operated mice were subjected to the same procedure but no ligation was made.

Sample Preparation:

Sampleprep ID:	SP001065
Sampleprep Summary:	Cardiac tissue samples were homogenized and sonicated in phosphate buffered saline (PBS) (pH 7.4). The Bicinchoninic acid (BCA) assay (Thermo Scientific) was used to determine protein concentration. A mixture of internal standards (10µl) in CHCl3: methanol (1:1) and 200µl of CHCl3/methanol (2:1) were added to each sample (ventricles- 50µg protein in 10µl; plasma- 10µl) before being briefly vortexed. Samples were first mixed (rotary mixer, 10 min), sonicated (water bath, 30 min at room temperature [RT]) then rested at RT (20 min). Samples were centrifuged (16,000 g, 10 min) and the supernatant was dried under a constant stream of nitrogen gas at 40°C. Extracted lipids were resuspended in 50µl H2O saturated butanol then sonicated (10 min), before 50 µl of methanol with 10mM ammonium was added. Resuspended extracts were centrifuged (3,350 g, 5 min), and the supernatant transferred to glass vials with Teflon insert caps and stored at -80°C until analyzed. Lipid extracts were thawed at RT for an hour, then sonicated in a water bath for 15 min at RT before analysis.

Sampleprep ID:

SP001065

Sampleprep Summary:

Cardiac tissue samples were homogenized and sonicated in phosphate buffered saline (PBS) (pH 7.4). The Bicinchoninic acid (BCA) assay (Thermo Scientific) was used to determine protein concentration. A mixture of internal standards (10µl) in CHCl3: methanol (1:1) and 200µl of CHCl3/methanol (2:1) were added to each sample (ventricles- 50µg protein in 10µl; plasma- 10µl) before being briefly vortexed. Samples were first mixed (rotary mixer, 10 min), sonicated (water bath, 30 min at room temperature [RT]) then rested at RT (20 min). Samples were centrifuged (16,000 g, 10 min) and the supernatant was dried under a constant stream of nitrogen gas at 40°C. Extracted lipids were resuspended in 50µl H2O saturated butanol then sonicated (10 min), before 50 µl of methanol with 10mM ammonium was added. Resuspended extracts were centrifuged (3,350 g, 5 min), and the supernatant transferred to glass vials with Teflon insert caps and stored at -80°C until analyzed. Lipid extracts were thawed at RT for an hour, then sonicated in a water bath for 15 min at RT before analysis.

Combined analysis:

Analysis ID	AN001667
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Agilent 6490
Column	Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um)
MS Type	ESI
MS instrument type	Triple quadrupole
MS instrument name	Agilent 6490 QQQ
Ion Mode	POSITIVE
Units	pmol/umol PC

Chromatography:

Chromatography ID:	CH001174
Instrument Name:	Agilent 6490
Column Name:	Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS001542
Analysis ID:	AN001667
Instrument Name:	Agilent 6490 QQQ
Instrument Type:	Triple quadrupole
MS Type:	ESI
Ion Mode:	POSITIVE