Summary of Study ST001044
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000700. The data can be accessed directly via it's Project DOI: 10.21228/M8697H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001044 |
| Study Title | PGC1-A effect on TCA enzymes |
| Study Type | Tracer time course in medium |
| Study Summary | 13C6-glucose used to track the effects of PGC1-A on RCC4 and Caki-1 metabolism |
| Institute | University of Alabama, Birmingham |
| Department | Urology |
| Last Name | Sudarshan |
| First Name | Sunil |
| Address | NA |
| sudarshan@uab.edu | |
| Phone | 000-000-0000 |
| Submit Date | 2018-08-31 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-03-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000700 |
| Project DOI: | doi: 10.21228/M8697H |
| Project Title: | PGC1-A induces TCA enzymes |
| Project Summary: | Demonstrate the experimental protocol of stable isotope tracer based metabolomic investigation of cell metabolism |
| Institute: | University of Alabama, Birmingham |
| Department: | Urology |
| Last Name: | Sudarshan |
| First Name: | Sunil |
| Address: | NA |
| Email: | sudarshan@uab.edu |
| Phone: | 000-000-0000 |
Subject:
| Subject ID: | SU001084 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | cell_type | protocol.id |
|---|---|---|---|
| SA070408 | 9_Caki-1_adGFP_13CGlc_rep1-cells-polar_GCMS_A | Caki-1 | adGFP_13CGlc |
| SA070409 | 10_Caki-1_adGFP_13CGlc_rep2-cells-polar_GCMS_A | Caki-1 | adGFP_13CGlc |
| SA070410 | 12_Caki-1_adPGC1A_13CGlc_rep2-cells-polar_GCMS_A | Caki-1 | adPGC1A_13CGlc |
| SA070411 | 11_Caki-1_adPGC1A_13CGlc_rep1-cells-polar_GCMS_A | Caki-1 | adPGC1A_13CGlc |
| SA070412 | 4_RCC4_adGFP_13CGlc_rep2-cells-polar_GCMS_A | RCC4 | adGFP_13CGlc |
| SA070413 | 3_RCC4_adGFP_13CGlc_rep1-cells-polar_GCMS_A | RCC4 | adGFP_13CGlc |
| SA070414 | 5_RCC4_adPGC1A_13CGlc_rep1-cells-polar_GCMS_A | RCC4 | adPGC1A_13CGlc |
| SA070415 | 6_RCC4_adPGC1A_13CGlc_rep2-cells-polar_GCMS_A | RCC4 | adPGC1A_13CGlc |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO001078 |
| Collection Summary: | Collection and quench of cells in tissue culture Media is extracted from plate. |
| Collection Protocol ID: | cell_quench_collection cells_media |
| Collection Protocol Filename: | WS_Fan_Quench_cell_tissue.pdf |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001098 |
| Treatment Summary: | Grown in unlabeled glucose and infected with adGFP. Grown in labeled glucose and infected with adGFP. Grown in unlabeled glucose and infected with adPGC1A. Grown in labeled glucose and infected with adPGC1A. |
| Treatment Protocol ID: | adGFP_12CGlc adGFP_13CGlc adPGC1A_12CGlc adPGC1A_13CGlc |
Sample Preparation:
| Sampleprep ID: | SP001091 |
| Sampleprep Summary: | Lipid extraction from cells. Polar extraction from cells. Protein extraction and quantification. |
| Sampleprep Protocol ID: | lipid_extraction polar_extraction protein_extraction |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf WS_Fan_Prot_Quant.pdf |
Combined analysis:
| Analysis ID | AN001708 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | GC-EI-MS, Thermo ITQ |
| Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| MS Type | EI |
| MS instrument type | GC-ITQ |
| MS instrument name | Thermo ITQ |
| Ion Mode | POSITIVE |
| Units | normalized peak area |
Chromatography:
| Chromatography ID: | CH001205 |
| Chromatography Summary: | Targeted GC |
| Instrument Name: | GC-EI-MS, Thermo ITQ |
| Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001581 |
| Analysis ID: | AN001708 |
| Instrument Name: | Thermo ITQ |
| Instrument Type: | GC-ITQ |
| MS Type: | EI |
| MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
| Ion Mode: | POSITIVE |
