Summary of Study ST001045
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000701. The data can be accessed directly via it's Project DOI: 10.21228/M82H46 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001045 |
| Study Title | FASN effect on HCT116 metabolism probed by 13C6-glucose tracer (part I) |
| Study Type | compare the metabolic pathways in control and stable FASN knockdown HCT116 cells using 13C Glc tracer |
| Study Summary | 13C6-glucose tracer used to track the effects of FASN on HCT116 CRC cells metabolism |
| Institute | University of Kentucky |
| Department | Markey Cancer Center |
| Last Name | Zaytseva |
| First Name | Kate |
| Address | B336-42, BBSRB 741 South Limestone Lexington, Kentucky 40536 |
| yyzayt2@uky.edu | |
| Phone | 000-000-0000 |
| Submit Date | 2018-08-31 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-03-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000701 |
| Project DOI: | doi: 10.21228/M82H46 |
| Project Title: | The role of Fatty Acid Synthase (FASN) in Colorectal Cancer (CRC) metastasis |
| Project Summary: | Determine the mechansims ( including alterations in metabolic pathways) that mediate the effect of FASN on metastasis in CRC |
| Institute: | University of Kentucky |
| Department: | Markey Cancer Center |
| Last Name: | Zaytseva |
| First Name: | Kate |
| Address: | B336-42, BBSRB 741 South Limestone Lexington, Kentucky 40536 |
| Email: | yyzayt2@uky.edu |
| Phone: | 000-000-0000 |
Subject:
| Subject ID: | SU001085 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | protocol.id |
|---|---|---|
| SA070416 | 5_ FASNsh_ no label Glc | 12CGlc_FASN_shRNA |
| SA070417 | 1_ NTC _no label Glc | 12CGlc_NTC_shRNA |
| SA070418 | 8_ FASNsh _13C Glc_3 | 13CGlc_FASN_shRNA |
| SA070419 | 7_ FASNsh _13C Glc_2 | 13CGlc_FASN_shRNA |
| SA070420 | 6_ FASNsh _13C Glc_1 | 13CGlc_FASN_shRNA |
| SA070421 | 2_ NTC _13C Glc_1 | 13CGlc_NTC_shRNA |
| SA070422 | 4_ NTC _13C Glc_3 | 13CGlc_NTC_shRNA |
| SA070423 | 3_ NTC _13C Glc_2 | 13CGlc_NTC_shRNA |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO001079 |
| Collection Summary: | Collection and quench of cells in tissue culture Media is extracted from plate. |
| Collection Protocol ID: | cell_quench_collection cells_media |
| Collection Protocol Filename: | WS_Fan_Quench_cell_tissue.pdf |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001099 |
| Treatment Summary: | Grown in unlabeled glucose and transfected with FASN shRNA. Grown in unlabeled glucose and transfected with non targeted shRNA. Grown in labeled glucose and transfected with FASN shRNA. Grown in labeled glucose and transfected with non targeted shRNA. |
| Treatment Protocol ID: | 12CGlc_FASN_shRNA 12CGlc_NTC_shRNA 13CGlc_FASN_shRNA 13CGlc_NTC_shRNA |
Sample Preparation:
| Sampleprep ID: | SP001092 |
| Sampleprep Summary: | Lipid extraction from cells. Polar extraction from cells. Protein extraction and quantification. |
| Sampleprep Protocol ID: | lipid_extraction polar_extraction protein_extraction |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf WS_Fan_Prot_Quant.pdf |
Combined analysis:
| Analysis ID | AN001709 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | GC-EI-MS, Thermo ITQ |
| Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| MS Type | EI |
| MS instrument type | GC-ITQ |
| MS instrument name | Thermo ITQ |
| Ion Mode | POSITIVE |
| Units | normalized peak area |
Chromatography:
| Chromatography ID: | CH001206 |
| Chromatography Summary: | Targeted GC |
| Instrument Name: | GC-EI-MS, Thermo ITQ |
| Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001582 |
| Analysis ID: | AN001709 |
| Instrument Name: | Thermo ITQ |
| Instrument Type: | GC-ITQ |
| MS Type: | EI |
| MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
| Ion Mode: | POSITIVE |
