Summary of Study ST001046

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000701. The data can be accessed directly via it's Project DOI: 10.21228/M82H46 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001046
Study TitleFASN effect on HCT116 metabolism probed by 13C6-glucose tracer (part II)
Study TypeCompare the metabolic pathways in control and stable FASN knockdown HCT116 cells using 13C Glc tracer
Study Summary13C6-glucose tracer used to track the effects of FASN on KM20 cells metabolism
University of Kentucky
DepartmentMarkey Cancer Center
Last NameZaytseva
First NameKate
AddressB336-42, BBSRB 741 South Limestone Lexington, Kentucky 40536
Submit Date2018-08-31
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2019-03-06
Release Version1
Kate Zaytseva Kate Zaytseva application/zip

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Project ID:PR000701
Project DOI:doi: 10.21228/M82H46
Project Title:The role of Fatty Acid Synthase (FASN) in Colorectal Cancer (CRC) metastasis
Project Summary:Determine the mechansims ( including alterations in metabolic pathways) that mediate the effect of FASN on metastasis in CRC
Institute:University of Kentucky
Department:Markey Cancer Center
Last Name:Zaytseva
First Name:Kate
Address:B336-42, BBSRB 741 South Limestone Lexington, Kentucky 40536


Subject ID:SU001086
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606


Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id
SA0704245_ FASNsh_ no label Glc12CGlc_FASN_shRNA
SA0704251_ NTC _no label Glc12CGlc_NTC_shRNA
SA0704268_ FASNsh _13C Glc_313CGlc_FASN_shRNA
SA0704277_ FASNsh _13C Glc_213CGlc_FASN_shRNA
SA0704286_ FASNsh _13C Glc_113CGlc_FASN_shRNA
SA0704292_ NTC _13C Glc_113CGlc_NTC_shRNA
SA0704304_ NTC _13C Glc_313CGlc_NTC_shRNA
SA0704313_ NTC _13C Glc_213CGlc_NTC_shRNA
Showing results 1 to 8 of 8


Collection ID:CO001080
Collection Summary:Collection and quench of cells in tissue culture Media is extracted from plate.
Collection Protocol ID:cell_quench_collection cells_media
Collection Protocol Filename:WS_Fan_Quench_cell_tissue.pdf
Sample Type:Cultured cells


Treatment ID:TR001100
Treatment Summary:Grown in unlabeled glucose and transfected with FASN shRNA. Grown in unlabeled glucose and transfected with non targeted shRNA. Grown in labeled glucose and transfected with FASN shRNA. Grown in labeled glucose and transfected with non targeted shRNA.
Treatment Protocol ID:12CGlc_FASN_shRNA 12CGlc_NTC_shRNA 13CGlc_FASN_shRNA 13CGlc_NTC_shRNA

Sample Preparation:

Sampleprep ID:SP001093
Sampleprep Summary:Lipid extraction from cells. Polar extraction from cells. Protein extraction and quantification.
Sampleprep Protocol ID:lipid_extraction polar_extraction protein_extraction
Sampleprep Protocol Filename:WS_Fan_Extract_Polar_Lipid_Prot.pdf

Combined analysis:

Analysis ID AN001710
Analysis type MS
Chromatography type GC
Chromatography system GC-EI-MS, Thermo ITQ
Column SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
MS Type EI
MS instrument type GC-ITQ
MS instrument name Thermo ITQ
Units normalized peak area


Chromatography ID:CH001207
Chromatography Summary:Targeted GC
Instrument Name:GC-EI-MS, Thermo ITQ
Column Name:SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane)
Chromatography Type:GC


MS ID:MS001583
Analysis ID:AN001710
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide).