Summary of Study ST001051
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000704. The data can be accessed directly via it's Project DOI: 10.21228/M8P96G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001051 |
| Study Title | High Salt Diet versus Low Salt Diet in rats |
| Study Summary | Two groups of rats were fed either a high salt diet or a low salt diet. This study aims to look at salt intake in correlation to altering other metabolites and the onset of hypertension |
| Institute | University of Toledo |
| Last Name | Chakraborty |
| First Name | Saroj |
| Address | 3000 Arlington Ave. Toledo, Ohio 43614-2598 |
| Saroj.Chakraborty@rockets.utoledo.edu | |
| Phone | 419.383.5174 |
| Submit Date | 2018-09-12 |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000704 |
| Project DOI: | doi: 10.21228/M8P96G |
| Project Title: | Untargetted MS analysis for Diet Comparison in Rats |
| Project Summary: | Primary metabolism was analyzed in 18 rat samples using untargetted GC-TOF MS analysis. |
| Institute: | University of California, Davis |
| Last Name: | Heil |
| First Name: | Hailey |
| Address: | 451 Health Science Drive Davis CA 95616 |
| Email: | hoheil@ucdavis.edu |
| Phone: | 9167471106 |
Subject:
| Subject ID: | SU001093 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Factors:
Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet |
|---|---|---|
| SA071391 | HS_S12549 | High Salt |
| SA071392 | HS_S12550 | High Salt |
| SA071393 | HS_S12542 | High Salt |
| SA071394 | HS_S12547 | High Salt |
| SA071395 | HS_S12548 | High Salt |
| SA071396 | HS_S12543 | High Salt |
| SA071397 | HS_S12545 | High Salt |
| SA071398 | HS_S12544 | High Salt |
| SA071399 | HS_S12546 | High Salt |
| SA071400 | LS_S12560 | Low Salt |
| SA071401 | LS_S12561 | Low Salt |
| SA071402 | LS_S12562 | Low Salt |
| SA071403 | LS_S12559 | Low Salt |
| SA071404 | LS_S12554 | Low Salt |
| SA071405 | LS_S12555 | Low Salt |
| SA071406 | LS_S12556 | Low Salt |
| SA071407 | LS_S12557 | Low Salt |
| SA071408 | LS_S12558 | Low Salt |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO001087 |
| Collection Summary: | Blood was collected from rats after completing either the high salt or low salt diet for a number of days. Rats fasted overnight before blood collection. Blood was then processed to submit only the plasma for GC-MS analysis. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR001107 |
| Treatment Summary: | 18 rats were placed into either a high salt diet group (2.0% NaCl) or a low salt diet group (0.3% NaCl), to create 2 groups of 9 rats each. The diet began at day 32 of life and continued for a number of days. |
Sample Preparation:
| Sampleprep ID: | SP001100 |
| Sampleprep Summary: | Samples were vortexed for 3 seconds to homogenize. Added 30 µL of each sample to a 1.5 mL eppendorf tube. Added 1 mL of extraction solvent (3:3:2 ACN:IPA:Water). Vortexed for 10 seconds. Shake for 6 minutes at 4°C. Centrifuged for 2 minutes at 14000 rcf. Aliquoted 2 x 475 µL portions of the supernatant into new tubes. Save one aliquote at -20°C for backup and dry the other in a centrivap to complete dryness. After the sample is dry, add 500 µL of 50:50 ACN:IPA. Centifuged for 2 minutes at 14000 rcf. Aliquoted 475 µL of supernatant to a new tube. Evaporate to complete dryness in centrivap. Submit for derivitzation. |
Combined analysis:
| Analysis ID | AN001720 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus HT TOF |
| Ion Mode | POSITIVE |
| Units | Peak Height |
Chromatography:
| Chromatography ID: | CH001216 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Temperature: | 50-330C |
| Flow Rate: | 1 mL min^(-1) |
| Injection Temperature: | 50°C ramped to 250°C by 12°C s-1 |
| Oven Temperature: | 50°C for 1 min, then ramped at 20°C min-1 to 330°C, held constant for 5 min |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001592 |
| Analysis ID: | AN001720 |
| Instrument Name: | Leco Pegasus HT TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
