Summary of Study ST001055

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000708. The data can be accessed directly via it's Project DOI: 10.21228/M8596T This work is supported by NIH grant, U2C- DK119886.

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Study IDST001055
Study TitleFatty acid profiling of liver tissue using GC-MS.
Study TypeTime Course
Study SummaryLiver tissue were harvested from wild type and CAR knockout mice treated for 48 or 72h with or without Car agonist (TCPOBOP).
Institute
Pennsylvania State University
LaboratoryOmiecinski Lab
Last NameOmiecinski
First NameCurt
Address101 Life Sciences Building
Emailcjo10@psu.edu, dmw178@psu.edu
Phone8148651572
Submit Date2018-09-11
Num Groups8
Total Subjects48
Num Males48
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2019-01-22
Release Version1
Curt Omiecinski Curt Omiecinski
https://dx.doi.org/10.21228/M8596T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000708
Project DOI:doi: 10.21228/M8596T
Project Title:Metabolic approaches reveal the role of CAR in energy metabolism
Project Type:Time Course
Project Summary:The constitutive androstane receptor (CAR; NR1I3) contributes important regulatory roles in biotransformation, xenobiotic transport function, energy metabolism and lipid homeostasis. In this investigation, global serum and liver tissue metabolomes were assessed analytically in wild type and CAR-null transgenic mice using NMR, GC/MS and UPLC/MS-MS-based metabolomics. Significantly, CAR activation increased serum levels of fatty acids, lactate, ketone bodies and tricarboxylic acid cycle products, whereas levels of phosphatidylcholine, sphingomyelin, amino acids and liver glucose were decreased following short-term activation of CAR. Mechanistically, quantitative mRNA analysis demonstrated significantly decreased expression of key gluconeogenic pathways, and increased expression of glucose utilization pathways, changes likely resulting from down-regulation of the hepatic glucose sensor and bi-directional transporter, Glut2. Short-term CAR activation also resulted in enhanced fatty acid synthesis and impaired β-oxidation. In summary, CAR contributes an expansive role regulating energy metabolism, significantly impacting glucose, and monocarboxylic acid, as well as fatty acid metabolism and lipid homeostasis, through receptor-mediated regulation of several genes in multiple associated pathways.
Institute:Pennsylvania State University
Laboratory:Omiecinski Lab
Last Name:Omiecinski
First Name:Curt
Address:101 Life Sciences Building
Email:cjo10@psu.edu, dmw178@psu.edu
Phone:8148651572

Subject:

Subject ID:SU001097
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Wild Type C57BL/6 and CAR Knockout
Age Or Age Range:Approximately 8 weeks old
Gender:Male
Animal Light Cycle:12 h
Animal Feed:ad libitum
Animal Water:ad libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Time Point (h) TCPOBOP (mg/kg)
SA071550CARKO_DMSO_48H_103CARKO 48 -
SA071551CARKO_DMSO_48H_104CARKO 48 -
SA071552CARKO_DMSO_48H_106CARKO 48 -
SA071553CARKO_DMSO_48H_102CARKO 48 -
SA071554CARKO_DMSO_48H_105CARKO 48 -
SA071555CARKO_DMSO_48H_101CARKO 48 -
SA071556CARKO_TCP_48H_206CARKO 48 2
SA071557CARKO_TCP_48H_207CARKO 48 2
SA071558CARKO_TCP_48H_205CARKO 48 2
SA071559CARKO_TCP_48H_204CARKO 48 2
SA071560CARKO_TCP_48H_201CARKO 48 2
SA071561CARKO_TCP_48H_202CARKO 48 2
SA071562CARKO_TCP_48H_203CARKO 48 2
SA071563CARKO_DMSO_72H_302CARKO 72 -
SA071564CARKO_DMSO_72H_301CARKO 72 -
SA071565CARKO_DMSO_72H_303CARKO 72 -
SA071566CARKO_DMSO_72H_304CARKO 72 -
SA071567CARKO_DMSO_72H_305CARKO 72 -
SA071568CARKO_DMSO_72H_306CARKO 72 -
SA071569CARKO_TCP_72H_405CARKO 72 2
SA071570CARKO_TCP_72H_406CARKO 72 2
SA071571CARKO_TCP_72H_404CARKO 72 2
SA071572CARKO_TCP_72H_403CARKO 72 2
SA071573CARKO_TCP_72H_402CARKO 72 2
SA071574CARKO_TCP_72H_401CARKO 72 2
SA071575WT_DMSO_48H_103WT 48 -
SA071576WT_DMSO_48H_102WT 48 -
SA071577WT_DMSO_48H_104WT 48 -
SA071578WT_DMSO_48H_105WT 48 -
SA071579WT_DMSO_48H_101WT 48 -
SA071580WT_DMSO_48H_107WT 48 -
SA071581WT_DMSO_48H_106WT 48 -
SA071582WT_TCP_48H_206WT 48 2
SA071583WT_TCP_48H_201WT 48 2
SA071584WT_TCP_48H_205WT 48 2
SA071585WT_TCP_48H_203WT 48 2
SA071586WT_TCP_48H_202WT 48 2
SA071587WT_TCP_48H_204WT 48 2
SA071588WT_DMSO_72H_302WT 72 -
SA071589WT_DMSO_72H_301WT 72 -
SA071590WT_DMSO_72H_303WT 72 -
SA071591WT_DMSO_72H_306WT 72 -
SA071592WT_DMSO_72H_304WT 72 -
SA071593WT_DMSO_72H_305WT 72 -
SA071594WT_TCP_72H_405WT 72 2
SA071595WT_TCP_72H_406WT 72 2
SA071596WT_TCP_72H_404WT 72 2
SA071597WT_TCP_72H_402WT 72 2
SA071598WT_TCP_72H_401WT 72 2
SA071599WT_TCP_72H_403WT 72 2
Showing results 1 to 50 of 50

Collection:

Collection ID:CO001091
Collection Summary:Liver tissue was snap-frozen in liquid nitrogen and stored at -80C.
Sample Type:Liver
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001111
Treatment Summary:Each mouse was treated with either a single dose of 2 mg/kg of TCPOBOP or the vehicle control via intraperitoneal injection for either 48 or 72h.

Sample Preparation:

Sampleprep ID:SP001104
Sampleprep Summary:Liver tissue was homogenized in a methanol-water mixture, dried and resuspended in phosphate buffer containing TSP-d4.
Sampleprep Protocol Filename:GCMS_FattyAcid_protocol.pdf
Processing Method:Homogenization

Combined analysis:

Analysis ID AN001724
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Agilent HP-5MS
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975C
Ion Mode POSITIVE
Units Peak Area

Chromatography:

Chromatography ID:CH001218
Instrument Name:Agilent 7890A
Column Name:Agilent HP-5MS
Chromatography Type:GC

MS:

MS ID:MS001594
Analysis ID:AN001724
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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