Summary of Study ST001060

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000711. The data can be accessed directly via it's Project DOI: 10.21228/M8S383 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001060
Study TitleNMR Metabolomics of Near-Term Fetal and Newborn Sheep Cardiac Tissue (part-I)
Study TypeComparison
Study SummaryCardiac tissue from near-term fetal and newborn sheep were compared via NMR metabolomic analysis
Institute
University of Florida
DepartmentBiochemistry & Molecular Biology
Last NameWalejko
First NameJacquelyn
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailjwalejko@ufl.edu
Phonena
Submit Date2018-08-31
Num Groups2
Total Subjects41
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2018-10-10
Release Version1
Jacquelyn Walejko Jacquelyn Walejko
https://dx.doi.org/10.21228/M8S383
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000711
Project DOI:doi: 10.21228/M8S383
Project Title:Multi-omics Approach Reveals Metabolic Changes in the Heart at Birth
Project Summary:During late gestation, the fetal heart primarily relies on glucose and lactate to support rapid growth and development. While numerous studies describe changes in heart metabolism a few weeks after birth to preferentially utilize fatty acids, little is known about metabolic changes of the heart within the first day following birth. Therefore, we used the ovine model of pregnancy to investigate metabolic differences between the near-term fetal and the newborn heart. Samples were collected for metabolomic, lipidomic, and transcriptomic approaches from the left and right ventricles and intraventricular septum in 7 fetuses at gestational day 142 and 7 newborn lambs on the day of birth. We observed greater abundance of metabolites involved in butanoate and propanoate metabolism, and glycolysis in the term fetal heart (FDR-corrected p<0.10) and differential expression in these pathways were confirmed with single-sample gene set enrichment analysis (ssGSEA) (FDR-corrected p<0.05). Immediately following birth, newborn hearts displayed enrichment in purine, fatty acid, and glycerophospholipid metabolic pathways, as well as oxidative phosphorylation with significant alterations in both lipids and metabolites to support transcriptomic findings. While other studies suggest a switch from carbohydrate metabolism to fatty acid metabolism in the neonatal heart in as early as 2 weeks following birth, our data show that this metabolic switch in the heart begins by the first day of postnatal life. A better understanding of metabolic alterations that occur in the heart following birth may improve treatment of neonates at risk for heart failure.
Institute:University of Florida
Department:Pharmacodynamics
Last Name:Keller-Wood
First Name:Maureen
Address:1345 SW Archer Rd, PO 100487, Gainesville, FL, 32610
Email:kellerwd@cop.ufl.edu
Phone:NA

Subject:

Subject ID:SU001102
Subject Type:Mammal
Subject Species:Ovis aries
Taxonomy ID:9940
Age Or Age Range:142 days gestation (fetal); Within 24 hours of birth (newborn)

Factors:

Subject type: Mammal; Subject species: Ovis aries (Factor headings shown in green)

mb_sample_id local_sample_id Age Area_heart Sex
SA0717391237B_LVFetal Left_ventricle Female
SA0717401481_LVFetal Left_ventricle Female
SA0717411562A_LVFetal Left_ventricle Male
SA0717421237A_LVFetal Left_ventricle Male
SA0717431555A_LVFetal Left_ventricle Male
SA0717441335B_LVFetal Left_ventricle Male
SA0717451335A_LVFetal Left_ventricle Male
SA0717461481_RVFetal Right_ventricle Female
SA0717471237B_RVFetal Right_ventricle Female
SA0717481562A_RVFetal Right_ventricle Male
SA0717491335A_RVFetal Right_ventricle Male
SA0717501335B_RVFetal Right_ventricle Male
SA0717511555A_RVFetal Right_ventricle Male
SA0717521237A_RVFetal Right_ventricle Male
SA0717531481_SFetal Septum Female
SA0717541237B_SFetal Septum Female
SA0717551237A_SFetal Septum Male
SA0717561335A_SFetal Septum Male
SA0717571562A_SFetal Septum Male
SA0717581335B_SFetal Septum Male
SA0717595562C_LVNewborn Left_ventricle Female
SA0717605562A_LVNewborn Left_ventricle Female
SA0717611767B_LVNewborn Left_ventricle Female
SA0717621767A_LVNewborn Left_ventricle Female
SA0717635562B_LVNewborn Left_ventricle Male
SA0717641462_LVNewborn Left_ventricle Male
SA0717651477_LVNewborn Left_ventricle Male
SA0717661767A_RVNewborn Right_ventricle Female
SA0717675562A_RVNewborn Right_ventricle Female
SA0717685562C_RVNewborn Right_ventricle Female
SA0717691767B_RVNewborn Right_ventricle Female
SA0717705562B_RVNewborn Right_ventricle Male
SA0717711462_RVNewborn Right_ventricle Male
SA0717721477B_RVNewborn Right_ventricle Male
SA0717735562A_SNewborn Septum Female
SA0717741767A_SNewborn Septum Female
SA0717755562C_SNewborn Septum Female
SA0717761767B_SNewborn Septum Female
SA0717771462_SNewborn Septum Male
SA0717781477B_SNewborn Septum Male
SA0717795562B_SNewborn Septum Male
Showing results 1 to 41 of 41

Collection:

Collection ID:CO001096
Collection Summary:Ewes were assigned to one of two groups: (1) a fetal group (n=7, 3 singleton and 2 multiple gestation pregnancies); (2) a newborn group (n=7, 2 singleton and 2 multiple gestation pregnancies). Heart tissue was collected from the right ventricle (RV), left ventricle (LV), and intraventricular septum (IVS) on the day of birth (newborn group) or at gestational day 142 (fetal group) and immediately frozen in liquid nitrogen. Samples were stored at -80 °C until data collection.
Sample Type:Cardiac tissue
Collection Method:Heart tissue was collected under sterile conditions and immediately frozen in liquid nitrogen
Collection Location:University of Florida
Storage Conditions:-80℃
Collection Vials:Cryovials
Storage Vials:Cryovials

Treatment:

Treatment ID:TR001116
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP001109
Sampleprep Summary:Heart tissue (20-50 mg) was cut and weighed on dry ice before being placed on a sterile agar plate. Thirty μL of deuterium oxide (D2O) was added to each tissue sample before being placed in a 4 mm HR-MAS rotor (Bruker Biospin, Billerica, MA, USA) and leftover D2O (from original 30 μL) was added to the rotor with a pipette after placement of tissue. The samples were kept on dry ice until data acquisition.

Analysis:

Analysis ID:AN001730
Laboratory Name:Advanced Magnetic Resonance Imaging and Spectroscopy Facility-University of Florida
Analysis Type:NMR
Acquisition Date:Aug-16
Software Version:Topspin 3.6
Operator Name:Jacquelyn Walejko
Num Factors:12
Num Metabolites:25
Units:Area under the curve

NMR:

NMR ID:NM000130
Analysis ID:AN001730
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Field Frequency Lock:H2O+D2O
Spectrometer Frequency:600 MHz
NMR Probe:4mm HR-MAS
NMR Solvent:D2O
NMR Tube Size:4mm HRMAS rotor
Shimming Method:Topshim
Pulse Sequence:noesypr1d
Water Suppression:Presaturation
Power Level:18 W
Receiver Gain:40.3
Offset Frequency:2819.74
Presaturation Power Level:3.98E-05
Temperature:4C
Number Of Scans:128
Dummy Scans:8
Acquisition Time:1.3598 sec
Relaxation Delay:2 sec
Spectral Width:10.0155 ppm
Num Data Points Acquired:16344
Real Data Points:32768
Line Broadening:2
Apodization:Exponential
Baseline Correction Method:Polynomial order 5
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