Summary of Study ST001064
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000714. The data can be accessed directly via it's Project DOI: 10.21228/M8CT1Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001064 |
| Study Title | Nude mice orthotopicly implanted with human glioma cell lines |
| Study Summary | Malignant gliomas are considered to be one of the deadliest human cancers, accounting for about 60% of all primary brain tumors. Cachexia is a complex metabolic derangement and muscle atrophy syndrome, which causes high mortalities in patients with advanced cancers including brain tumors. However, cachexia symptoms induced by gliomas and mechanisms underlying muscle atrophy are unclear. Herein, we developed a glioma cachexia model using nude mice orthotopicly implanted with two glioma cell lines (WHO II CHG5 and WHO IV U87). U87 mice developed more severe cachexia symptoms than CHG5 mice, including more evident anorexia, greater body weight loss and mortality. Unlike non-central nervous system cancer cachexia, glioma cachexia did not induce remarkable systemic inflammation but massive multi-organ atrophy. It also caused significantly decreased skeletal muscle mass and strength, which were associated with down-regulated myosin and AKT, and up-regulated AMPK, FOXO and Atrogin1. Interestingly, expressions of MuRF1, MyoD1, eIF3f, desmin and vimentin were not significantly changed. Consistently, NMR-based metabolomic analyses revealed pronounced metabolic derangements in cachectic gastrocnemius relative to controls. Glucose, glycerol, 3-hydroxybutyrate and glycine were remarkably down-regulated, whereas largely released amino acids due to proteolysis including glutamate, arginine, leucine and isoleucine were up-regulated in cachectic gastrocnemius. Moreover, glucose and lipid metabolism, protein biosynthesis and amino acid metabolism were disturbed dramatically in both glioma-bearing mice. U87 mice showed more changed metabolite levels and altered metabolic pathways. This work uncovers malignant grade-dependent glioma cachexia symptoms and metabolic derangements of skeletal muscle for the first time, and provides hints for new therapeutic approaches. |
| Institute | Xiamen University |
| Last Name | Cui |
| First Name | Pengfei |
| Address | 422 siming south road |
| edmundcui@126.com | |
| Phone | 15060796092 |
| Submit Date | 2018-09-22 |
| Analysis Type Detail | NMR |
| Release Date | 2020-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000714 |
| Project DOI: | doi: 10.21228/M8CT1Z |
| Project Title: | Cachexia symptoms induced by Gliomas - NMR metabolomics |
| Project Summary: | Malignant gliomas are considered to be one of the deadliest human cancers, accounting for about 60% of all primary brain tumors. Cachexia is a complex metabolic derangement and muscle atrophy syndrome, which causes high mortalities in patients with advanced cancers including brain tumors. However, cachexia symptoms induced by gliomas and mechanisms underlying muscle atrophy are unclear. Herein, we developed a glioma cachexia model using nude mice orthotopicly implanted with two glioma cell lines (WHO II CHG5 and WHO IV U87). U87 mice developed more severe cachexia symptoms than CHG5 mice, including more evident anorexia, greater body weight loss and mortality. Unlike non-central nervous system cancer cachexia, glioma cachexia did not induce remarkable systemic inflammation but massive multi-organ atrophy. It also caused significantly decreased skeletal muscle mass and strength, which were associated with down-regulated myosin and AKT, and up-regulated AMPK, FOXO and Atrogin1. Interestingly, expressions of MuRF1, MyoD1, eIF3f, desmin and vimentin were not significantly changed. Consistently, NMR-based metabolomic analyses revealed pronounced metabolic derangements in cachectic gastrocnemius relative to controls. Glucose, glycerol, 3-hydroxybutyrate and glycine were remarkably down-regulated, whereas largely released amino acids due to proteolysis including glutamate, arginine, leucine and isoleucine were up-regulated in cachectic gastrocnemius. Moreover, glucose and lipid metabolism, protein biosynthesis and amino acid metabolism were disturbed dramatically in both glioma-bearing mice. U87 mice showed more changed metabolite levels and altered metabolic pathways. This work uncovers malignant grade-dependent glioma cachexia symptoms and metabolic derangements of skeletal muscle for the first time, and provides hints for new therapeutic approaches. |
| Institute: | Xiamen University |
| Department: | Chemistry |
| Last Name: | Cui |
| First Name: | Pengfei |
| Address: | 422 siming south road |
| Email: | edmundcui@126.com |
| Phone: | 86-15060796092 |
Subject:
| Subject ID: | SU001175 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Celltype | Treatment |
|---|---|---|---|
| SA077661 | C2 | CHG5 | lowgrade |
| SA077662 | C4 | CHG5 | lowgrade |
| SA077663 | C6 | CHG5 | lowgrade |
| SA077664 | C1 | CHG5 | lowgrade |
| SA077665 | C5 | CHG5 | lowgrade |
| SA077666 | C3 | CHG5 | lowgrade |
| SA077667 | H7 | HEB | control |
| SA077668 | H2 | HEB | control |
| SA077669 | H3 | HEB | control |
| SA077670 | H4 | HEB | control |
| SA077671 | H6 | HEB | control |
| SA077672 | H1 | HEB | control |
| SA077673 | H5 | HEB | control |
| SA077674 | U6 | U87 | highgrade |
| SA077675 | U5 | U87 | highgrade |
| SA077676 | U1 | U87 | highgrade |
| SA077677 | U2 | U87 | highgrade |
| SA077678 | U3 | U87 | highgrade |
| SA077679 | U4 | U87 | highgrade |
| Showing results 1 to 19 of 19 |
Collection:
| Collection ID: | CO001169 |
| Collection Summary: | Muscles were rapidly dissected, weighted and frozen in liquid nitrogen and stored at -80 °C until analyses. |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR001189 |
| Treatment Summary: | Right caudate nuclei of mice were separately injected with 2.0 μL of the suspension of 2.0 × 105 of HEB, CHG5 and U87 cells. muscles were rapidly dissected, weighted and frozen. |
Sample Preparation:
| Sampleprep ID: | SP001182 |
| Sampleprep Summary: | Generally, gastrocnemius muscle samples were homogenized after adding ice-cold methanol, chloroform and water at a volume ratio of 4:4:2.85 to obtain a two-phase extract. |
Analysis:
| Analysis ID: | AN001829 |
| Analysis Type: | NMR |
| Num Factors: | 3 |
NMR:
| NMR ID: | NM000142 |
| Analysis ID: | AN001829 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 850 MHz |
