Summary of Study ST001072

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000715. The data can be accessed directly via it's Project DOI: 10.21228/M8839T This work is supported by NIH grant, U2C- DK119886.

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Study IDST001072
Study TitleThe in-gel proteomic characterization of exercise-induced sweat - (part III)
Study SummaryThe in-gel proteomic characterization of exercise-induced sweat to add depth to the evaluation of the sweat protein content.
Institute
UES Inc;Air Force Research Laboratory
Last NameHarshman
First NameSean
Address2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433
Emailsean.harshman.ctr@us.af.mil
Phone937-938-3785
Submit Date2018-10-08
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2018-12-11
Release Version1
Sean Harshman Sean Harshman
https://dx.doi.org/10.21228/M8839T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000715
Project DOI:doi: 10.21228/M8839T
Project Title:The proteomic and metabolomic characterization of exercise-induced sweat
Project Summary:Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte correlation with human performance parameters.
Institute:UES Inc;Air Force Research Laboratory
Last Name:Harshman
First Name:Sean
Address:2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433
Email:sean.harshman.ctr@us.af.mil
Phone:937-938-3785

Subject:

Subject ID:SU001116
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Gel
SA072380InGel_#10175ug_Peptide
SA072381InGel_#9175ug_Peptide
SA072382InGel_#12175ug_Peptide
SA072383InGel_#13175ug_Peptide
SA072384InGel_#1175ug_Peptide
SA072385InGel_#8175ug_Peptide
SA072386InGel_#11175ug_Peptide
SA072387InGel_#3175ug_Peptide
SA072388InGel_#7175ug_Peptide
SA072389InGel_#4175ug_Peptide
SA072390InGel_#2175ug_Peptide
SA072391InGel_#5175ug_Peptide
SA072392InGel_#6175ug_Peptide
SA072393InGel_K2ug_Protein
SA072394InGel_L2ug_Protein
SA072395InGel_M2ug_Protein
SA072396InGel_P2ug_Protein
SA072397InGel_J2ug_Protein
SA072398InGel_S2ug_Protein
SA072399InGel_N2ug_Protein
SA072400InGel_B2ug_Protein
SA072401InGel_D2ug_Protein
SA072402InGel_C2ug_Protein
SA072403InGel_A2ug_Protein
SA072404InGel_E2ug_Protein
SA072405InGel_F2ug_Protein
SA072406InGel_H2ug_Protein
SA072407InGel_G2ug_Protein
SA072408InGel_I2ug_Protein
Showing results 1 to 29 of 29

Collection:

Collection ID:CO001110
Collection Summary:Sweat collected from the forearms of participants.
Sample Type:Sweat

Treatment:

Treatment ID:TR001130
Treatment Summary:Sweat induced by exercise on a treadmill.

Sample Preparation:

Sampleprep ID:SP001123
Sampleprep Summary:Sweat samples were collected, pooled, and frozen on liquid nitrogen. Following, frozen samples were lyophilized and frozen until analysis. Samples were run in 2 SDS-PAGE gels, excised, digested, and analyzed by LS-MS-MS. Gel 1 was loaded based on 175ug of sweat peptides (Factor) and Gel 2 was loaded based on 2ug of protein (Factor).

Combined analysis:

Analysis ID AN001754
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS
Column Easy-Spray PepMap 50μm x 150μm, 100Å, 2μm
MS Type ESI
MS instrument type Single quadrupole
MS instrument name Thermo LTQ XL
Ion Mode POSITIVE
Units Spectral Counts

Chromatography:

Chromatography ID:CH001238
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Easy-Spray PepMap 50μm x 150μm, 100Å, 2μm
Chromatography Type:Reversed phase

MS:

MS ID:MS001622
Analysis ID:AN001754
Instrument Name:Thermo LTQ XL
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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