Summary of Study ST001077

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000721. The data can be accessed directly via it's Project DOI: 10.21228/M8GM4Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST001077
Study TitleMulti-platform metabolomics data from Ndufs4-/- skeletal muscles (part-II)
Study TypeTargeted LC-MS/MS of Ndufs4-/- soleus muscles
Study SummaryTargeted analysis of amino acids and acylcarnitines in mouse Ndufs4-/- and WT soleus muscles
Institute
North-West University
Last NameLouw
First NameRoan
AddressHofman Street
EmailRoan.Louw@nwu.ac.za
Phone+27 18 299 4074
Submit Date2018-10-16
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Roan Louw Roan Louw
https://dx.doi.org/10.21228/M8GM4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000721
Project DOI:doi: 10.21228/M8GM4Z
Project Title:Multi-platform metabolomics data from Ndufs4-/- skeletal muscles
Project Type:Multi-platform metabolomics study
Project Summary:Multi-platform metabolomics analysis of white quadriceps and soleus skeletal muscles from the Ndufs4 knockout (Ndufs4-/-) mouse model of human Leigh syndrome.
Institute:North-West University
Last Name:Louw
First Name:Roan
Address:Hofman Street
Email:Roan.Louw@nwu.ac.za
Phone:+27 18 299 4074

Subject:

Subject ID:SU001121
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Ndufs4, https://www.jax.org/strai n/02705 8
Age Or Age Range:45-50 days
Gender:Male
Animal Animal Supplier:Jackson Laboratory (ME, USA)
Animal Light Cycle:12:12 h
Animal Feed:Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub
Animal Water:ad libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA072563Ms_14Ndufs4-/-
SA072564Ms_17Ndufs4-/-
SA072565Ms_19Ndufs4-/-
SA072566Ms_12Ndufs4-/-
SA072567Ms_8Ndufs4-/-
SA072568Ms_4Ndufs4-/-
SA072569Ms_5Ndufs4-/-
SA072570Ms_21Ndufs4-/-
SA072571Ms_10Ndufs4-/-
SA072572Ms_24Ndufs4-/-
SA072573Ms_37Ndufs4-/-
SA072574Ms_38Ndufs4-/-
SA072575Ms_40Ndufs4-/-
SA072576Ms_35Ndufs4-/-
SA072577Ms_34Ndufs4-/-
SA072578Ms_2Ndufs4-/-
SA072579Ms_26Ndufs4-/-
SA072580Ms_29Ndufs4-/-
SA072581Ms_23Ndufs4-/-
SA072582Ms_39WT
SA072583Ms_13WT
SA072584Ms_15WT
SA072585Ms_16WT
SA072586Ms_18WT
SA072587Ms_11WT
SA072588Ms_9WT
SA072589Ms_3WT
SA072590Ms_6WT
SA072591Ms_7WT
SA072592Ms_20WT
SA072593Ms_22WT
SA072594Ms_32WT
SA072595Ms_33WT
SA072596Ms_36WT
SA072597Ms_31WT
SA072598Ms_30WT
SA072599Ms_25WT
SA072600Ms_27WT
SA072601Ms_28WT
SA072602Ms_1WT
Showing results 1 to 40 of 40

Collection:

Collection ID:CO001115
Collection Summary:Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used.
Sample Type:Muscle

Treatment:

Treatment ID:TR001135
Treatment Summary:The animals did not receive any treatment

Sample Preparation:

Sampleprep ID:SP001128
Sampleprep Summary:Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform).

Combined analysis:

Analysis ID AN001759
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1200
Column Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410 QQQ
Ion Mode POSITIVE
Units Normalized peak area

Chromatography:

Chromatography ID:CH001243
Instrument Name:Agilent 1200
Column Name:Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001627
Analysis ID:AN001759
Instrument Name:Agilent 6410 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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