Summary of Study ST001085

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000721. The data can be accessed directly via it's Project DOI: 10.21228/M8GM4Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001085
Study TitleMulti-platform metabolomics data from Ndufs4-/- skeletal muscles (part-IV)
Study TypeUnargeted GC-TOF-MS of Ndufs4-/- soleus muscles
Study SummaryUntargeted GC-TOF-MS analysis of Ndufs4-/- and WT mouse soleus muscles.
Institute
North-West University
Last NameLouw
First NameRoan
AddressHofman Street
EmailRoan.Louw@nwu.ac.za
Phone+27 18 299 4074
Submit Date2018-10-18
Raw Data AvailableYes
Raw Data File Type(s)peg
Analysis Type DetailGC-MS
Release Date2019-01-22
Release Version1
Roan Louw Roan Louw
https://dx.doi.org/10.21228/M8GM4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000721
Project DOI:doi: 10.21228/M8GM4Z
Project Title:Multi-platform metabolomics data from Ndufs4-/- skeletal muscles
Project Type:Multi-platform metabolomics study
Project Summary:Multi-platform metabolomics analysis of white quadriceps and soleus skeletal muscles from the Ndufs4 knockout (Ndufs4-/-) mouse model of human Leigh syndrome.
Institute:North-West University
Last Name:Louw
First Name:Roan
Address:Hofman Street
Email:Roan.Louw@nwu.ac.za
Phone:+27 18 299 4074

Subject:

Subject ID:SU001129
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Ndufs4, https://www.jax.org/strai n/02705 8
Age Or Age Range:45-50 days
Gender:Male
Animal Animal Supplier:Jackson Laboratory (ME, USA)
Animal Light Cycle:12:12 h
Animal Feed:Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub
Animal Water:ad libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA073389Ms_14Ndufs4-/-
SA073390Ms_17Ndufs4-/-
SA073391Ms_19Ndufs4-/-
SA073392Ms_12Ndufs4-/-
SA073393Ms_8Ndufs4-/-
SA073394Ms_4Ndufs4-/-
SA073395Ms_5Ndufs4-/-
SA073396Ms_21Ndufs4-/-
SA073397Ms_10Ndufs4-/-
SA073398Ms_24Ndufs4-/-
SA073399Ms_37Ndufs4-/-
SA073400Ms_38Ndufs4-/-
SA073401Ms_40Ndufs4-/-
SA073402Ms_35Ndufs4-/-
SA073403Ms_34Ndufs4-/-
SA073404Ms_2Ndufs4-/-
SA073405Ms_26Ndufs4-/-
SA073406Ms_29Ndufs4-/-
SA073407Ms_23Ndufs4-/-
SA073408Ms_39WT
SA073409Ms_13WT
SA073410Ms_15WT
SA073411Ms_16WT
SA073412Ms_18WT
SA073413Ms_11WT
SA073414Ms_9WT
SA073415Ms_3WT
SA073416Ms_6WT
SA073417Ms_7WT
SA073418Ms_20WT
SA073419Ms_22WT
SA073420Ms_32WT
SA073421Ms_33WT
SA073422Ms_36WT
SA073423Ms_31WT
SA073424Ms_30WT
SA073425Ms_25WT
SA073426Ms_27WT
SA073427Ms_28WT
SA073428Ms_1WT
Showing results 1 to 40 of 40

Collection:

Collection ID:CO001123
Collection Summary:Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used.
Sample Type:Muscle

Treatment:

Treatment ID:TR001143
Treatment Summary:The animals did not receive any treatment.

Sample Preparation:

Sampleprep ID:SP001136
Sampleprep Summary:Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform).
Sampleprep Protocol Filename:GC_soleus_sample_prep_protocol.pdf

Combined analysis:

Analysis ID AN001770
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus HT TOF
Ion Mode POSITIVE
Units Normalized peak area

Chromatography:

Chromatography ID:CH001250
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS001636
Analysis ID:AN001770
Instrument Name:Leco Pegasus HT TOF
Instrument Type:GC-TOF
MS Type:EI
Ion Mode:POSITIVE
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