Summary of Study ST001085
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000721. The data can be accessed directly via it's Project DOI: 10.21228/M8GM4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001085 |
Study Title | Multi-platform metabolomics data from Ndufs4-/- skeletal muscles (part-IV) |
Study Type | Unargeted GC-TOF-MS of Ndufs4-/- soleus muscles |
Study Summary | Untargeted GC-TOF-MS analysis of Ndufs4-/- and WT mouse soleus muscles. |
Institute | North-West University |
Last Name | Louw |
First Name | Roan |
Address | Hofman Street |
Roan.Louw@nwu.ac.za | |
Phone | +27 18 299 4074 |
Submit Date | 2018-10-18 |
Raw Data Available | Yes |
Raw Data File Type(s) | peg |
Analysis Type Detail | GC-MS |
Release Date | 2019-01-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000721 |
Project DOI: | doi: 10.21228/M8GM4Z |
Project Title: | Multi-platform metabolomics data from Ndufs4-/- skeletal muscles |
Project Type: | Multi-platform metabolomics study |
Project Summary: | Multi-platform metabolomics analysis of white quadriceps and soleus skeletal muscles from the Ndufs4 knockout (Ndufs4-/-) mouse model of human Leigh syndrome. |
Institute: | North-West University |
Last Name: | Louw |
First Name: | Roan |
Address: | Hofman Street |
Email: | Roan.Louw@nwu.ac.za |
Phone: | +27 18 299 4074 |
Subject:
Subject ID: | SU001129 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
Age Or Age Range: | 45-50 days |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
Animal Light Cycle: | 12:12 h |
Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
Animal Water: | ad libitum |
Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Genotype |
---|---|---|
SA073389 | Ms_14 | Ndufs4-/- |
SA073390 | Ms_17 | Ndufs4-/- |
SA073391 | Ms_19 | Ndufs4-/- |
SA073392 | Ms_12 | Ndufs4-/- |
SA073393 | Ms_8 | Ndufs4-/- |
SA073394 | Ms_4 | Ndufs4-/- |
SA073395 | Ms_5 | Ndufs4-/- |
SA073396 | Ms_21 | Ndufs4-/- |
SA073397 | Ms_10 | Ndufs4-/- |
SA073398 | Ms_24 | Ndufs4-/- |
SA073399 | Ms_37 | Ndufs4-/- |
SA073400 | Ms_38 | Ndufs4-/- |
SA073401 | Ms_40 | Ndufs4-/- |
SA073402 | Ms_35 | Ndufs4-/- |
SA073403 | Ms_34 | Ndufs4-/- |
SA073404 | Ms_2 | Ndufs4-/- |
SA073405 | Ms_26 | Ndufs4-/- |
SA073406 | Ms_29 | Ndufs4-/- |
SA073407 | Ms_23 | Ndufs4-/- |
SA073408 | Ms_39 | WT |
SA073409 | Ms_13 | WT |
SA073410 | Ms_15 | WT |
SA073411 | Ms_16 | WT |
SA073412 | Ms_18 | WT |
SA073413 | Ms_11 | WT |
SA073414 | Ms_9 | WT |
SA073415 | Ms_3 | WT |
SA073416 | Ms_6 | WT |
SA073417 | Ms_7 | WT |
SA073418 | Ms_20 | WT |
SA073419 | Ms_22 | WT |
SA073420 | Ms_32 | WT |
SA073421 | Ms_33 | WT |
SA073422 | Ms_36 | WT |
SA073423 | Ms_31 | WT |
SA073424 | Ms_30 | WT |
SA073425 | Ms_25 | WT |
SA073426 | Ms_27 | WT |
SA073427 | Ms_28 | WT |
SA073428 | Ms_1 | WT |
Showing results 1 to 40 of 40 |
Collection:
Collection ID: | CO001123 |
Collection Summary: | Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used. |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR001143 |
Treatment Summary: | The animals did not receive any treatment. |
Sample Preparation:
Sampleprep ID: | SP001136 |
Sampleprep Summary: | Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform). |
Sampleprep Protocol Filename: | GC_soleus_sample_prep_protocol.pdf |
Combined analysis:
Analysis ID | AN001770 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus HT TOF |
Ion Mode | POSITIVE |
Units | Normalized peak area |
Chromatography:
Chromatography ID: | CH001250 |
Instrument Name: | Agilent 7890A |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS001636 |
Analysis ID: | AN001770 |
Instrument Name: | Leco Pegasus HT TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |