Summary of Study ST001092
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000708. The data can be accessed directly via it's Project DOI: 10.21228/M8596T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001092 |
| Study Title | Metabolic profiling by NMR analysis in liver aqueous extract samples |
| Study Type | Time Course |
| Study Summary | Liver tissue were harvested from wild type and CAR knockout mice treated for 48 or 72h with or without TCPOBOP. |
| Institute | Pennsylvania State University |
| Laboratory | Omiecinski Lab |
| Last Name | Omiecinski |
| First Name | Curt |
| Address | 101 Life Sciences Building |
| cjo10@psu.edu, dmw178@psu.edu | |
| Phone | 8148651572 |
| Submit Date | 2018-09-11 |
| Num Groups | 8 |
| Total Subjects | 48 |
| Num Males | 48 |
| Raw Data Available | Yes |
| Analysis Type Detail | NMR |
| Release Date | 2019-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000708 |
| Project DOI: | doi: 10.21228/M8596T |
| Project Title: | Metabolic approaches reveal the role of CAR in energy metabolism |
| Project Type: | Time Course |
| Project Summary: | The constitutive androstane receptor (CAR; NR1I3) contributes important regulatory roles in biotransformation, xenobiotic transport function, energy metabolism and lipid homeostasis. In this investigation, global serum and liver tissue metabolomes were assessed analytically in wild type and CAR-null transgenic mice using NMR, GC/MS and UPLC/MS-MS-based metabolomics. Significantly, CAR activation increased serum levels of fatty acids, lactate, ketone bodies and tricarboxylic acid cycle products, whereas levels of phosphatidylcholine, sphingomyelin, amino acids and liver glucose were decreased following short-term activation of CAR. Mechanistically, quantitative mRNA analysis demonstrated significantly decreased expression of key gluconeogenic pathways, and increased expression of glucose utilization pathways, changes likely resulting from down-regulation of the hepatic glucose sensor and bi-directional transporter, Glut2. Short-term CAR activation also resulted in enhanced fatty acid synthesis and impaired β-oxidation. In summary, CAR contributes an expansive role regulating energy metabolism, significantly impacting glucose, and monocarboxylic acid, as well as fatty acid metabolism and lipid homeostasis, through receptor-mediated regulation of several genes in multiple associated pathways. |
| Institute: | Pennsylvania State University |
| Laboratory: | Omiecinski Lab |
| Last Name: | Omiecinski |
| First Name: | Curt |
| Address: | 101 Life Sciences Building |
| Email: | cjo10@psu.edu, dmw178@psu.edu |
| Phone: | 8148651572 |
Subject:
| Subject ID: | SU001136 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Wild Type C57BL/6 and CAR Knockout |
| Age Or Age Range: | Approximately 8 weeks old |
| Gender: | Male |
| Animal Light Cycle: | 12 h |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype | Time Point (h) | TCPOBOP (mg/kg) |
|---|---|---|---|---|
| SA074484 | WT_DMSO_48H_101 | WT | 48 | - |
| SA074485 | WT_DMSO_48H_106 | WT | 48 | - |
| SA074486 | WT_DMSO_48H_105 | WT | 48 | - |
| SA074487 | WT_DMSO_48H_102 | WT | 48 | - |
| SA074488 | WT_DMSO_48H_103 | WT | 48 | - |
| SA074489 | WT_DMSO_48H_104 | WT | 48 | - |
| SA074490 | WT_TCP_48H_206 | WT | 48 | 2 |
| SA074491 | WT_TCP_48H_205 | WT | 48 | 2 |
| SA074492 | WT_TCP_48H_202 | WT | 48 | 2 |
| SA074493 | WT_TCP_48H_201 | WT | 48 | 2 |
| SA074494 | WT_TCP_48H_204 | WT | 48 | 2 |
| SA074495 | WT_TCP_48H_203 | WT | 48 | 2 |
| SA074496 | WT_DMSO_72H_306 | WT | 72 | - |
| SA074497 | WT_DMSO_72H_305 | WT | 72 | - |
| SA074498 | WT_DMSO_72H_301 | WT | 72 | - |
| SA074499 | WT_DMSO_72H_304 | WT | 72 | - |
| SA074500 | WT_DMSO_72H_302 | WT | 72 | - |
| SA074501 | WT_DMSO_72H_303 | WT | 72 | - |
| SA074502 | WT_TCP_72H_406 | WT | 72 | 2 |
| SA074503 | WT_TCP_72H_405 | WT | 72 | 2 |
| SA074504 | WT_TCP_72H_403 | WT | 72 | 2 |
| SA074505 | WT_TCP_72H_401 | WT | 72 | 2 |
| SA074506 | WT_TCP_72H_402 | WT | 72 | 2 |
| SA074507 | WT_TCP_72H_404 | WT | 72 | 2 |
| Showing results 1 to 24 of 24 |
Collection:
| Collection ID: | CO001130 |
| Collection Summary: | Liver tissue was snap-frozen in liquid nitrogen and stored at -80C. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001150 |
| Treatment Summary: | Each mouse was treated with either a single dose of 2 mg/kg of CAR agonist (TCPOBOP) or the vehicle control via intraperitoneal injection. |
Sample Preparation:
| Sampleprep ID: | SP001143 |
| Sampleprep Summary: | Liver tissue was homogenized in a methanol-water mixture, dried and resuspended in phosphate buffer containing TSP-d4. |
| Sampleprep Protocol Filename: | NMR_metabolomics_protocol.pdf |
| Processing Method: | Homogenization |
| Processing Storage Conditions: | 4? |
| Extraction Method: | Methanol/Water |
| Extract Storage: | On ice |
| Sample Resuspension: | D2O |
| Sample Spiking: | TSP-d4 |
Analysis:
| Analysis ID: | AN001778 |
| Laboratory Name: | Omiecinski Lab |
| Analysis Type: | NMR |
| Num Factors: | 4 |
NMR:
| NMR ID: | NM000134 |
| Analysis ID: | AN001778 |
| Instrument Name: | Bruker AVANCE DRX |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | Other |
| Spectrometer Frequency: | 600 MHz |
| NMR Solvent: | D2O |
