Summary of Study ST001101
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000737. The data can be accessed directly via it's Project DOI: 10.21228/M8DM5Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001101 |
| Study Title | Identification of potential sepsis biomarkers in burn injury conditions. |
| Study Summary | 12 mice were subjected to one of four treatment groups: no burn injury:no injection, burn injury:no infection, no burn injury:infection, and burn injury:infection. The researchers in this study are looking for metabolites in serum that could indicate sepsis in mice with burn injuries. |
| Institute | Texas Tech University |
| Department | Department of Biological Sciences |
| Last Name | Elmassry |
| First Name | Moamen |
| Address | 2901 Main St, Lubbock, TX 79409 |
| moamen.elmassry@ttu.edu | |
| Phone | 806-742-2715 |
| Submit Date | 2018-11-29 |
| Num Groups | 4 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000737 |
| Project DOI: | doi: 10.21228/M8DM5Q |
| Project Title: | Identifying sepsis biomarkes, using GC-MS, in mice with burn injuries |
| Project Summary: | 12 mice were subjected to one of four treatment groups. After completion of treatment, mouse serum was taken from each mouse, prepared for analysis, and they studied using GC-MS analysis. |
| Institute: | University of California, Davis |
| Department: | Genome and Biomedical Sciences Facility |
| Laboratory: | WCMC Metabolomics Core |
| Last Name: | Fiehn |
| First Name: | Oliver |
| Address: | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
| Email: | ofiehn@ucdavis.edu |
| Phone: | (530) 754-8258 |
Subject:
| Subject ID: | SU001145 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Burn Status | Infected |
|---|---|---|---|
| SA075053 | F | Burned | No |
| SA075054 | D | Burned | No |
| SA075055 | E | Burned | No |
| SA075056 | K | Burned | Yes |
| SA075057 | J | Burned | Yes |
| SA075058 | L | Burned | Yes |
| SA075059 | A | Healthy | No |
| SA075060 | C | Healthy | No |
| SA075061 | B | Healthy | No |
| SA075062 | G | Healthy | Yes |
| SA075063 | H | Healthy | Yes |
| SA075064 | I | Healthy | Yes |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO001139 |
| Collection Summary: | Blood were aseptically collected from mice directly from the heart then left at room temperature for a few hours to clot, then centrifuged to separate the serum. Serum was then packed on dry ice and sent to the WCMC center in Davis, CA. |
| Sample Type: | Serum |
Treatment:
| Treatment ID: | TR001159 |
| Treatment Summary: | Mice were placed into 1 of 4 treatment groups, with three mice in each group. The first group contained uninjured mice who were not infected. The second group contained thermally injured mice who were not infected. The third group contained mice that were uninjured and infected with Pseudomonas aeruginosa. The fourth group of mice were thermally injured and infected with Pseudomonas aeruginosa. |
Sample Preparation:
| Sampleprep ID: | SP001152 |
| Sampleprep Summary: | 30µL of plasma was aliquoted into a 1.5 mL eppendorph tube. 1 mL of 3:3:2 ACN:H20:IPA was added to each sample. Samples were vortexed for 10 seconds and then shaken at 4°C for 5 minutes using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 x 475 µL aliquots were removed, one dried down and submitted to derivitization, the other frozen at -20°C for backup. Samples were derivatized by adding 10 µL of MeOX and then shaken at 30°C for 1.5 hours using a orbital mixing plate. 91 µL of MSTFA + FAMES mixutre is added to each sample. Samples were shaken at 37°C for 30 minutes. Samples submitted to GC analysis. |
Combined analysis:
| Analysis ID | AN001790 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Peak Height |
Chromatography:
| Chromatography ID: | CH001264 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Temperature: | 50-330°C |
| Flow Rate: | 1 mL min^(-1) |
| Injection Temperature: | 50°C ramped to 250°C by 12°C s-1 |
| Oven Temperature: | 50°C for 1 min, then ramped at 20°C min-1 to 330°C, held constant for 5 min |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001652 |
| Analysis ID: | AN001790 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
