Summary of Study ST001105
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000739. The data can be accessed directly via it's Project DOI: 10.21228/M85675 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001105 |
Study Title | Retinal ganglion cells lipid profiling |
Study Summary | The aim of this project was to characterize lipid profiles of the retinal ganglion cells (RGCs) with different regenerative capacity. RGCs were FACS-sorted (7,000 cells/sample) from mice of OPN4 Cre tdT and Thy1-CFP genotype. The treatment conditions included intact, optic nerve crush (ONC) and ONC plus CNTF to promote regeneration. Samples were then subject to lipid extraction and analyzed using LC-MS/MS, followed by identification and relative quantification in LipidSearch software. |
Institute | University of Miami |
Department | Ophthalmology, Bascom Palmer Eye Institute |
Laboratory | Sanjoy K. Bhattacharya |
Last Name | Bhattacharya |
First Name | Sanjoy |
Address | 1638 NW 10th Ave, Miami, FL 33136 |
sbhattacharya@med.miami.edu | |
Phone | +1 (305) 4824103 |
Submit Date | 2018-11-26 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2019-01-22 |
Release Version | 1 |
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Project:
Project ID: | PR000739 |
Project DOI: | doi: 10.21228/M85675 |
Project Title: | Retinal ganglion cells lipid profiling |
Project Summary: | The aim of this project was to characterize lipid profiles of the retinal ganglion cells (RGCs) with different regenerative capacity. RGCs were FACS-sorted (7,000 cells/sample) from mice of OPN4 Cre tdT and Thy1-CFP genotype. The treatment conditions included intact, optic nerve crush (ONC) and ONC plus CNTF to promote regeneration. Samples were then subject to lipid extraction and analyzed using LC-MS/MS, followed by identification and relative quantification in LipidSearch software. |
Institute: | University of Miami |
Department: | Ophthalmology, Bascom Palmer Eye Institute |
Laboratory: | Sanjoy K. Bhattacharya |
Last Name: | Bhattacharya |
First Name: | Sanjoy |
Address: | 900 NW 17th St, Miami, FL 33136 |
Email: | sbhattacharya@med.miami.edu |
Phone: | +1 (305) 4824103 |
Funding Source: | NEI/NIH U01EY027257 |
Contributors: | Kevin (Kyung) Park, Sanjoy K. Bhattacharya, Ana Carolina Ayupe Oliveira Beckedorff, Anna Trzeciecka |
Subject:
Subject ID: | SU001150 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment |
---|---|---|
SA075191 | KPF_QC3 | - |
SA075192 | KPF_QC1 | - |
SA075193 | KPF_QC4 | - |
SA075194 | KPF_QC2 | - |
SA075195 | KPF_QC5 | - |
SA075196 | KPF_QC6 | - |
SA075197 | KPF_18 | CNTF +ONC |
SA075198 | KPF_27 | CNTF +ONC |
SA075199 | KP_A3 | CNTF +ONC |
SA075200 | KP_A10 | CNTF +ONC |
SA075201 | KPF_17 | CNTF +ONC |
SA075202 | KP_A11 | CNTF +ONC |
SA075203 | KP_A4 | CNTF +ONC |
SA075204 | KPF_26 | CNTF +ONC |
SA075205 | KPF_11 | CNTF +ONC |
SA075206 | KPF_4 | CNTF +ONC |
SA075207 | KPF_3 | CNTF +ONC |
SA075208 | KPF_10 | CNTF +ONC |
SA075209 | KPF_5 | GFP +ONC |
SA075210 | KPF_13 | GFP +ONC |
SA075211 | KPF_6 | GFP +ONC |
SA075212 | KPF_12 | GFP +ONC |
SA075213 | KPF_20 | GFP +ONC |
SA075214 | KPF_19 | GFP +ONC |
SA075215 | KPF_24 | Intact |
SA075216 | KP_A8 | Intact |
SA075217 | KP_A9 | Intact |
SA075218 | KPF_8 | Intact |
SA075219 | KPF_9 | Intact |
SA075220 | KPF_22 | Intact |
SA075221 | KPF_16 | Intact |
SA075222 | KPF_23 | Intact |
SA075223 | KPF_1 | Intact |
SA075224 | KPF_25 | Intact |
SA075225 | KPF_2 | Intact |
SA075226 | KPF_15 | Intact |
SA075227 | KP_A2 | Intact |
SA075228 | KP_A1 | Intact |
SA075235 | KPF_29 | tdT +ONC |
SA075236 | KPF_28 | tdT +ONC |
SA075237 | KP_A6 | tdT +ONC |
SA075238 | KP_A12 | tdT +ONC |
SA075239 | KP_A13 | tdT +ONC |
SA075240 | KP_A5 | tdT +ONC |
SA075229 | KPF_7 | WT |
SA075230 | KPF_14 | WT |
SA075231 | KP_A14 | WT |
SA075232 | KP_A7 | WT |
SA075233 | KPF_21 | WT |
SA075234 | KPF_30 | WT |
Showing results 1 to 50 of 50 |
Collection:
Collection ID: | CO001144 |
Collection Summary: | As described in attached method file. |
Sample Type: | retinal ganglion cells |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001164 |
Treatment Summary: | As described in attached method file. |
Treatment Protocol Filename: | Methods.docx |
Sample Preparation:
Sampleprep ID: | SP001157 |
Sampleprep Summary: | As described in attached method file. |
Sampleprep Protocol Filename: | Methods.docx |
Extract Storage: | -20℃ |
Combined analysis:
Analysis ID | AN001798 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Accela 600 |
Column | Thermo Acclaim C30 (150 x 2.1mm,3um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | main area |
Chromatography:
Chromatography ID: | CH001269 |
Chromatography Summary: | As described in attached method file. |
Methods Filename: | Methods.docx |
Instrument Name: | Thermo Accela 600 |
Column Name: | Thermo Acclaim C30 (150 x 2.1mm,3um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001659 |
Analysis ID: | AN001798 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | UNSPECIFIED |