Summary of Study ST001110
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000741. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ2Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001110 |
| Study Title | Investigating link between metabolism and circadian rhythm in Drosophila melanogaster |
| Study Summary | We are investigating the link between metabolism, circadian rhythm, and a high-fat diet with an emphasis on the role of metabolites that affect protein post translational modifications. We've hypothesized that protein modifications, which are critical to circadian clock functions, can be affected by different metabolic profiles, such as an excess or lack of fat in the diet, that ultimately regulate changes in circadian biology. |
| Institute | University of California, Davis |
| Department | College of Biological Sciences |
| Last Name | Contreras |
| First Name | Adam |
| Address | 202 Life Sciences Building, Kleiber Hall Drive, Davis, CA, 95616 |
| ajcon@ucdavis.edu | |
| Phone | 512-393-4773 |
| Submit Date | 2018-10-19 |
| Total Subjects | 96 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000741 |
| Project DOI: | doi: 10.21228/M8WQ2Z |
| Project Title: | Untargeted GC-MS Analysis in Drosophila melanogaster |
| Project Summary: | Untargeted GC-MS Analysis in Drosophila melanogaster. |
| Institute: | University of California, Davis |
| Department: | Genome and Biomedical Sciences Facility |
| Laboratory: | WCMC Metabolomics Core |
| Last Name: | Fiehn |
| First Name: | Oliver |
| Address: | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
| Email: | ofiehn@ucdavis.edu |
| Phone: | (530) 754-8258 |
Subject:
| Subject ID: | SU001155 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Species Group: | Insects |
Factors:
Subject type: Insect; Subject species: Drosophila melanogaster (Factor headings shown in green)
| mb_sample_id | local_sample_id | Organ | Treatment |
|---|---|---|---|
| SA075645 | Bodies Zt10 - Rep2_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075646 | Bodies Zt10 - Rep3_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075647 | Bodies Zt10 - Rep5_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075648 | Bodies Zt10 - Rep1_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075649 | Bodies Zt10 - Rep4_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075650 | Bodies Zt4 - Rep5_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075651 | Bodies Zt4 - Rep2_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075652 | Bodies Zt4 - Rep3_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075653 | Bodies Zt4 - Rep4_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075654 | Bodies Zt10 - Rep6_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075655 | Bodies Zt4 - Rep6_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075656 | Bodies Zt16 - Rep3_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075657 | Bodies Zt22 - Rep3_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075658 | Bodies Zt22 - Rep4_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075659 | Bodies Zt22 - Rep5_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075660 | Bodies Zt22 - Rep6_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075661 | Bodies Zt22 - Rep2_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075662 | Bodies Zt22 - Rep1_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075663 | Bodies Zt4 - Rep1_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075664 | Bodies Zt16 - Rep4_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075665 | Bodies Zt16 - Rep5_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075666 | Bodies Zt16 - Rep6_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075667 | Bodies Zt16 - Rep2_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075668 | Bodies Zt16 - Rep1_NFD | Body Tissue | Cornmeal Drosphila Diet |
| SA075669 | Bodies Zt16 - Rep4_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075670 | Bodies Zt16 - Rep5_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075671 | Bodies Zt16 - Rep3_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075672 | Bodies Zt16 - Rep2_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075673 | Bodies Zt16 - Rep1_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075674 | Bodies Zt16 - Rep6_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075675 | Bodies Zt22 - Rep1_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075676 | Bodies Zt22 - Rep5_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075677 | Bodies Zt22 - Rep4_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075678 | Bodies Zt22 - Rep3_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075679 | Bodies Zt22 - Rep2_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075680 | Bodies Zt10 - Rep6_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075681 | Bodies Zt4 - Rep1_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075682 | Bodies Zt4 - Rep5_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075683 | Bodies Zt4 - Rep4_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075684 | Bodies Zt4 - Rep3_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075685 | Bodies Zt4 - Rep2_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075686 | Bodies Zt4 - Rep6_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075687 | Bodies Zt10 - Rep1_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075688 | Bodies Zt10 - Rep5_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075689 | Bodies Zt10 - Rep4_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075690 | Bodies Zt10 - Rep3_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075691 | Bodies Zt10 - Rep2_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075692 | Bodies Zt22 - Rep6_HFD | Body Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075693 | Heads Zt4 - Rep2_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075694 | Heads Zt16 - Rep5_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075695 | Heads Zt16 - Rep4_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075696 | Heads Zt16 - Rep3_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075697 | Heads Zt16 - Rep2_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075698 | Heads Zt16 - Rep6_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075699 | Heads Zt22 - Rep1_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075700 | Heads Zt22 - Rep5_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075701 | Heads Zt22 - Rep4_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075702 | Heads Zt22 - Rep3_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075703 | Heads Zt22 - Rep2_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075704 | Heads Zt16 - Rep1_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075705 | Heads Zt10 - Rep6_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075706 | Heads Zt4 - Rep5_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075707 | Heads Zt4 - Rep4_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075708 | Heads Zt4 - Rep3_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075709 | Heads Zt22 - Rep6_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075710 | Heads Zt4 - Rep6_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075711 | Heads Zt10 - Rep1_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075712 | Heads Zt10 - Rep5_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075713 | Heads Zt10 - Rep4_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075714 | Heads Zt10 - Rep3_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075715 | Heads Zt10 - Rep2_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075716 | Heads Zt4 - Rep1_NFD | Head Tissue | Cornmeal Drosphila Diet |
| SA075717 | Heads Zt4 - Rep1_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075718 | Heads Zt10 - Rep2_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075719 | Heads Zt10 - Rep3_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075720 | Heads Zt10 - Rep4_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075721 | Heads Zt10 - Rep5_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075722 | Heads Zt10 - Rep1_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075723 | Heads Zt4 - Rep6_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075724 | Heads Zt4 - Rep2_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075725 | Heads Zt4 - Rep3_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075726 | Heads Zt4 - Rep4_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075727 | Heads Zt4 - Rep5_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075728 | Heads Zt22 - Rep6_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075729 | Heads Zt10 - Rep6_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075730 | Heads Zt22 - Rep2_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075731 | Heads Zt22 - Rep3_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075732 | Heads Zt22 - Rep4_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075733 | Heads Zt22 - Rep5_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075734 | Heads Zt16 - Rep1_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075735 | Heads Zt22 - Rep1_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075736 | Heads Zt16 - Rep2_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075737 | Heads Zt16 - Rep6_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075738 | Heads Zt16 - Rep3_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075739 | Heads Zt16 - Rep4_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075740 | Heads Zt16 - Rep5_HFD | Head Tissue | Cornmeal Drosphila Diet + 15% coconut Oil |
| SA075741 | Standard Food | N/A | Cornmeal Drosphila Diet |
| SA075742 | High Fat Food | N/A | Cornmeal Drosphila Diet + 15% coconut Oil |
| Showing results 1 to 98 of 98 |
Collection:
| Collection ID: | CO001149 |
| Collection Summary: | After each subject completed the treatment for the required time, the head of the fly was removed from the body over dry ice. Each portion of tissue was placed in a 1.5 mL tube and stored at -80°C until shipped to the WCMC for analysis. |
| Sample Type: | Tissue |
| Volumeoramount Collected: | Variable, depending on the size of the fly. |
| Storage Conditions: | -80℃ |
| Collection Vials: | 1.5 mL |
| Storage Vials: | 1.5 mL |
| Collection Tube Temp: | Dry Ice, -78.5°C |
Treatment:
| Treatment ID: | TR001169 |
| Treatment Summary: | 48 flies were split into two treatment groups, with 24 flies in each group. Group 1 consisted of a High Fat Diet (HFD, Cornmeal diet + 15% coconut oil) and Group 2 consisted of a Non-Fat Diet (NFD, Cornmeal). Within each group, flies were placed in one of four smaller groups, depending on how long they would be on either the HFD or NFD. These groups are ZT4, ZT10, ZT16, ZT22. Each of these smaller groups consisted of 6 flies, to create 4 groups of 6 flies each in both of the diet treatments. |
Sample Preparation:
| Sampleprep ID: | SP001162 |
| Sampleprep Summary: | Add 1 mL of 3:3:2 Acetonitrile:Isopropyl Alcohol:LC-MS Grade Water to each preweighed sample. Vortex each sample for 5 seconds. Shake at 4°C for 5 minutes using the Orbital Mixing Chilling/Heating Plate. Keep remaining samples on ice before shaking. Centrifuge samples for 2 minutes at 14000 rcf. Aliquot two 450 µL portions of the supernatant. Submit one to analysis and save one as backup at -20°C. To the submitted sample, dry in Labconco Centrivap. Submit to derivatization. |
| Sampleprep Protocol Filename: | SOP_blood-GCTOF-11082012_332.pdf |
| Sampleprep Protocol Comments: | Extraction solvent is prepared previously. Begin procedure at Sample Preparation under step 3. |
| Sample Derivatization: | Add 10 µL of prepared MeOX solution to each sample. Shake for 1.5 hours at 30°C. Add 91 µL of prepared MSTFA + FAME solution to each sample. Shake for 30 minutes at 37°C. |
Combined analysis:
| Analysis ID | AN001804 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus HT TOF |
| Ion Mode | POSITIVE |
| Units | Peak Height |
Chromatography:
| Chromatography ID: | CH001271 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001662 |
| Analysis ID: | AN001804 |
| Instrument Name: | Leco Pegasus HT TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250°C |
| Ionization Energy: | -70 eV |
| Dataformat: | .CDF |
| Resolution Setting: | 17 spectra s-1 |
