Summary of Study ST001112
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000743. The data can be accessed directly via it's Project DOI: 10.21228/M8N68W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001112 |
| Study Title | The nutrition value of fish fillet is related to fish maturation or fish age? |
| Study Summary | Integrated analysis of transcriptomics and metabolomics in Blunt Snout Bream. With the improvement of living standards, people’s demand for food nutrient is getting higher and higher. Fish is one kind of protein-rich food and is increasingly favored by consumers. It has been well recognized that flesh composition of fish is closely related to its maturation and growth stages, but few researches have explored these differences. Besides, hormone residues in fish after artificial inducing reproduction also attract consumers’ concern. In this study, we try to address these concerns by using a combination of transcriptomics and metabolomics analysis to identify the key pathways, genes, and metabolites regulation which may affect flesh nutrition of one typical aquaculture species in China, blunt snout bream (Megalobrama amblycephala). |
| Institute | Huazhong Agricultural University |
| Last Name | Guan |
| First Name | Ningnan |
| Address | College of Fisheries,Huazhong Agricultural University 1 Shizishan Road Hongshan District Wuhan, Hubei |
| ningnan06@163.com | |
| Phone | +8613007100773 |
| Submit Date | 2017-09-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | bcd |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-01-22 |
| Release Version | 1 |
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Project:
| Project ID: | PR000743 |
| Project DOI: | doi: 10.21228/M8N68W |
| Project Title: | The nutrition value of fish fillet is related to fish maturation or fish age |
| Project Type: | metabolomic |
| Project Summary: | With the improvement of living standards, people’s demand for food nutrient is getting higher and higher. Fish is one kind of protein-rich food and is increasingly favored by consumers. It has been well recognized that flesh composition of fish is closely related to its maturation and growth stages, but few researches have explored these differences. Besides, hormone residues in fish after artificial inducing reproduction also attract consumers’ concern. In this study, we try to address these concerns by using a combination of transcriptomics and metabolomics analysis to identify the key pathways, genes, and metabolites regulation which may affect flesh nutrition of one typical aquaculture species in China, blunt snout bream (Megalobrama amblycephala). |
| Institute: | Huazhong Agricultural University |
| Department: | Breeding and Reproduction of Ministry of Education |
| Laboratory: | Key Laboratory of Agricultural Animal Genetics |
| Last Name: | Guan |
| First Name: | Ningnan |
| Address: | College of Fisheries,Huazhong Agricultural University 1 Shizishan Road Hongshan District Wuhan, Hubei |
| Email: | ningnan06@163.com |
| Phone: | +8613007100773 |
| Funding Source: | National Natural Science Foundation of China |
| Project Comments: | none |
| Publications: | none |
| Contributors: | Shiming-Wan, Bowen-Zhao, Laifang-Zhou, Weizhuo-Zhang |
Subject:
| Subject ID: | SU001157 |
| Subject Type: | Fish |
| Subject Species: | Megalobrama amblycephala |
| Taxonomy ID: | 75352 |
Factors:
Subject type: Fish; Subject species: Megalobrama amblycephala (Factor headings shown in green)
| mb_sample_id | local_sample_id | age(years) | mature | spawned | hormone treatment |
|---|---|---|---|---|---|
| SA075861 | M-GroupI_23 | 1 | No | No | Yes |
| SA075862 | M-GroupI_19 | 1 | No | No | Yes |
| SA075863 | M-GroupI_14 | 1 | No | No | Yes |
| SA075864 | M-GroupI_28 | 1 | No | No | Yes |
| SA075865 | M-GroupI_36 | 1 | No | No | Yes |
| SA075866 | M-GroupI_45 | 1 | No | No | Yes |
| SA075867 | M-GroupI_41 | 1 | No | No | Yes |
| SA075868 | M-GroupI_10 | 1 | No | No | Yes |
| SA075869 | M-GroupI_32 | 1 | No | No | Yes |
| SA075870 | M-GroupI_6 | 1 | No | No | Yes |
| SA075871 | M-GroupII_20 | 2 | No | No | Yes |
| SA075872 | M-GroupII_15 | 2 | No | No | Yes |
| SA075873 | M-GroupII_11 | 2 | No | No | Yes |
| SA075874 | M-GroupII_7 | 2 | No | No | Yes |
| SA075875 | M-GroupII_29 | 2 | No | No | Yes |
| SA075876 | M-GroupII_24 | 2 | No | No | Yes |
| SA075877 | M-GroupII_33 | 2 | No | No | Yes |
| SA075878 | M-GroupII_42 | 2 | No | No | Yes |
| SA075879 | M-GroupII_46 | 2 | No | No | Yes |
| SA075880 | M-GroupII_37 | 2 | No | No | Yes |
| SA075881 | M-GroupIII_25 | 2 | Yes | No | No |
| SA075882 | M-GroupIII_21 | 2 | Yes | No | No |
| SA075883 | M-GroupIII_12 | 2 | Yes | No | No |
| SA075884 | M-GroupIII_30 | 2 | Yes | No | No |
| SA075885 | M-GroupIII_17 | 2 | Yes | No | No |
| SA075886 | M-GroupIII_34 | 2 | Yes | No | No |
| SA075887 | M-GroupIII_8 | 2 | Yes | No | No |
| SA075888 | M-GroupIII_47 | 2 | Yes | No | No |
| SA075889 | M-GroupIII_43 | 2 | Yes | No | No |
| SA075890 | M-GroupIII_39 | 2 | Yes | No | No |
| SA075891 | M-GroupIV_31 | 2 | Yes | Yes | Yes |
| SA075892 | M-GroupIV_26 | 2 | Yes | Yes | Yes |
| SA075893 | M-GroupIV_18 | 2 | Yes | Yes | Yes |
| SA075894 | M-GroupIV_35 | 2 | Yes | Yes | Yes |
| SA075895 | M-GroupIV_22 | 2 | Yes | Yes | Yes |
| SA075896 | M-GroupIV_48 | 2 | Yes | Yes | Yes |
| SA075897 | M-GroupIV_9 | 2 | Yes | Yes | Yes |
| SA075898 | M-GroupIV_13 | 2 | Yes | Yes | Yes |
| SA075899 | M-GroupIV_44 | 2 | Yes | Yes | Yes |
| SA075900 | M-GroupIV_40 | 2 | Yes | Yes | Yes |
| Showing results 1 to 40 of 40 |
Collection:
| Collection ID: | CO001151 |
| Collection Summary: | The gonads of fish were collected to identify its sex of 1-year-old fish by traditional histological analysis and the muscle samples were only selected from the females (group Ⅰ). As to 2-year-old M. amblycephala, the muscle samples were firstly collected from individuals, and then the gonads were collected to do the histological analysis. Individuals with ovary development before stage Ⅴ were recognized as non-mature females (group Ⅱ), while individuals with ovary development at stage Ⅴdwere recognized as mature females (group ⅡI). For group IV, the individuals which normally spawned during artificial reproduction, were put back to tank and then muscle samples were collected after 24 hours. For each group, muscle samples from 10 individuals were collected. After the collection, muscle samples were frozen in liquid nitrogen immediately and then transferred them into -80℃ for further study. |
| Sample Type: | Muscle and Gonad tissue |
Treatment:
| Treatment ID: | TR001171 |
| Treatment Summary: | Samples were divided into four groups: 1-year-old immature M. amblycephala females (group Ⅰ), 2-year-old immature M. amblycephala females (group Ⅰ), 2-year-old mature females before the injection of hormones (group Ⅲ) and 2-year-old females after 24 hours of their successfully spawning by injecting oxytocin (group ⅣanThe artificial oxytocin implementation procedure was as follows. Firstly, the fish were intraperitoneally injected with a luteinizing hormone-releasing hormone from the basal part of the pectoral fin (LRH-A2) (1 µg/kg fish body weight). After 10 h, the second injection was given (0.5 ml/kg body weight) (each ml contained LRH-A2 4 µg, human chorionic gonadotropin (HCG) 500 U, and domperidone (DOM) 5 mg of solution). |
Sample Preparation:
| Sampleprep ID: | SP001164 |
| Sampleprep Summary: | Muscle tissues about 25 mg from each individual were homogenized in 800 µL cold methanol and water (v/v = 1:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). 400 µL supernatant was collected after centrifugation (25000 g, 4 °C, 10 min). The supernatant in the eppendorf tube was removed and the sediment in the tube was dried. Homogenized the sediment again in 800 µL cold dichloromethane and methanol (v/v = 3:1), after centrifuging and then collected 200 µL supernatant for QC (quality control) sample (25000 g, 4 °C, 10 min). The supernatant was evacuated under vacuum, re-collected 100µL supernatant after centrifugation (25000 g, 4 °C, 10 min) for subsequent detection. |
Combined analysis:
| Analysis ID | AN001807 | AN001808 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Unspecified | Unspecified |
| Chromatography system | Waters | Waters |
| Column | ion information | ion information |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 XS | Waters Synapt G2 XS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | none | none |
Chromatography:
| Chromatography ID: | CH001273 |
| Instrument Name: | Waters |
| Column Name: | ion information |
| Chromatography Type: | Unspecified |
MS:
| MS ID: | MS001665 |
| Analysis ID: | AN001807 |
| Instrument Name: | Waters Synapt G2 XS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS001666 |
| Analysis ID: | AN001808 |
| Instrument Name: | Waters Synapt G2 XS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
