Summary of Study ST001113
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000744. The data can be accessed directly via it's Project DOI: 10.21228/M8HD5K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001113 |
| Study Title | Exposure to Oral Contraceptives Alters Human Endometrial Stem Cells Culture Media Metabolomics |
| Study Summary | Although the effects of oral contraceptives (OCs) in the endometrium has been well established, its influence in the production and metabolism of endometrial mesenchymal stem cells (EnMSC) remains unclear. Therefore, we analyzed the effect of OCs in the EnMSC secretome by culture media quantitative metabolomics. The EnMSC were collected from menstrual shedding of five donors (OC group, n=3; control, non-OC group, n=2) and cultured for three passages. Cells characterization was performed by flow cytometry, and culture media was collected at the end of each passage for further quantitative metabolomics. The metabolites with higher discriminant power for sample classification were Alanine, PC aa C30:0, c4-OH-Pro, PC aa C32:2, PC ae C32:2, PC ae C40:2, glycine and PC ae C32:1 for the OC group, whereas PC aa C36:6, PC aa C34:4, SM OH C16:1, SM C26:0, PC aa C38:0, serine and PC aa C36:5 were representative of the non-OC group. This panel of metabolites showed 98% of sensitivity in sample classification according with respective groups. Altered concentrations of metabolites may be an effect of OC hormonal properties on EnMSC metabolism. Thus, this metabolomic approach could assist in the management of future stem cell therapies according to patients’ specific responses to hormone treatments. |
| Institute | Universidade Federal de Sao Paulo |
| Last Name | Cordeiro |
| First Name | Fernanda |
| Address | Rua Embau, 231, Vila Clementino |
| fernandabertuccez85@gmail.com | |
| Phone | 11996667402 |
| Submit Date | 2018-01-16 |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2019-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000744 |
| Project DOI: | doi: 10.21228/M8HD5K |
| Project Title: | Exposure to Oral Contraceptives Alters Human Endometrial Stem Cells Culture Media Metabolomics |
| Project Summary: | The project analyzed the effect of oral contraceptives in the metabolism of endometrial mesenchmal stem cells, via culture media quantitative metabolomics. |
| Institute: | Universidade Federal de Sao Paulo |
| Last Name: | Bertuccez Cordeiro |
| First Name: | Fernanda |
| Address: | Rua Embau, 231, Vila Clementino, Sao Paulo, São Paulo, 04039-060, Brazil |
| Email: | fernandabertuccez85@gmail.com |
| Phone: | 5511996667402 |
Subject:
| Subject ID: | SU001158 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Woman |
Factors:
Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Study Group |
|---|---|---|
| SA075910 | R12 | non-OC |
| SA075911 | R8 | non-OC |
| SA075912 | R7 | non-OC |
| SA075913 | R9 | non-OC |
| SA075914 | R10 | non-OC |
| SA075915 | R11 | non-OC |
| SA075901 | R1 | OC |
| SA075902 | R15 | OC |
| SA075903 | R14 | OC |
| SA075904 | R13 | OC |
| SA075905 | R6 | OC |
| SA075906 | R2 | OC |
| SA075907 | R3 | OC |
| SA075908 | R4 | OC |
| SA075909 | R5 | OC |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO001152 |
| Collection Summary: | The endometrial mesenchymal stem cells (EnMSC) were collected from menstrual shedding, isolated and cultured in vitro from donors who used or not oral contraceptives. The EnMSC were grown up until the second passage (P2) and 24h before the end of each passage (P0, P1 and P2) the culture medium was completely replaced for the collection. The samples were centrifuged at 400 × g for 5 minutes, filtered with 0,2 µm filter and stored at -80 ºC until the metabolomics analysis without any extraction method. |
| Sample Type: | Stem cells culture media |
Treatment:
| Treatment ID: | TR001172 |
| Treatment Summary: | Culture media samples were collected by the end of each passage, kept under -20C until the moment of analysis. |
Sample Preparation:
| Sampleprep ID: | SP001165 |
| Sampleprep Summary: | The endometrial mesenchymal stem cells (EnMSC) were collected from menstrual shedding, isolated and cultured in vitro from donors who used or not oral contraceptives. The EnMSC were grown up until the second passage (P2) and 24h before the end of each passage (P0, P1 and P2) the culture medium was completely replaced for the collection. The samples were centrifuged at 400 × g for 5 minutes, filtered with 0,2 µm filter and stored at -80 ºC until the metabolomics analysis without any extraction method. |
Combined analysis:
| Analysis ID | AN001809 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | None |
| Column | none |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 4000 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | umol |
Chromatography:
| Chromatography ID: | CH001274 |
| Instrument Name: | None |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS001667 |
| Analysis ID: | AN001809 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | UNSPECIFIED |
