Summary of Study ST001115

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000746. The data can be accessed directly via it's Project DOI: 10.21228/M87X0C This work is supported by NIH grant, U2C- DK119886.

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Study IDST001115
Study TitleGrowth cone-enriched lipidome of embryonic to early postnatal mouse brain
Study SummaryA growth cone (GC) is a part of a neuron considered a hub for axon growth, motility and guidance functions. Unraveling the molecular composition of GCs and events through which active GCs transition to terminal synapses is of importance to developmental and regenerative neuroscience research. Here, we present a dataset on the lipid profiling of the growth cone-enriched fractions derived from E18, P0, P3, P6 and P9 C57BL/6J mouse brains. For comparison, we analyzed non-growth cone membranes.
Institute
University of Miami
DepartmentOphthalmology, Bascom Palmer Eye Institute
LaboratorySanjoy K. Bhattacharya
Last NameBhattacharya
First NameSanjoy
Address1638 NW 10th Ave, Miami, FL 33136
Emailsbhattacharya@med.miami.edu
Phone+1 (305) 4824103
Submit Date2018-11-26
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2019-01-16
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M87X0C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000746
Project DOI:doi: 10.21228/M87X0C
Project Title:Growth cone-enriched lipidome of embryonic to early postnatal mouse brain
Project Summary:A growth cone (GC) is a part of a neuron considered a hub for axon growth, motility and guidance functions. Unraveling the molecular composition of GCs and events through which active GCs transition to terminal synapses is of importance to developmental and regenerative neuroscience research. Here, we present a dataset on the lipid profiling of the growth cone-enriched fractions derived from E18, P0, P3, P6 and P9 C57BL/6J mouse brains. For comparison, we analyzed non-growth cone membranes.
Institute:University of Miami
Department:Ophthalmology, Bascom Palmer Eye Institute
Laboratory:Sanjoy K. Bhattacharya
Last Name:Bhattacharya
First Name:Sanjoy
Address:1638 NW 10th Ave, Miami, FL 33136
Email:sbhattacharya@med.miami.edu
Phone:+1 (305) 4824103
Contributors:Sanjoy K. Bhattacharya, Anna M. Trzeciecka

Subject:

Subject ID:SU001161
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Animal Animal Supplier:The Jackson Laboratory

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Fraction Age
SA076141GC_E18_1GC E18
SA076142GC_E18_6GC E18
SA076143GC_E18_5GC E18
SA076144GC_E18_2GC E18
SA076145GC_E18_3GC E18
SA076146GC_E18_4GC E18
SA076147GC_P0_6GC P0
SA076148GC_P0_5GC P0
SA076149GC_P0_2GC P0
SA076150GC_P0_1GC P0
SA076151GC_P0_4GC P0
SA076152GC_P0_3GC P0
SA076153GC_P3_6GC P3
SA076154GC_P3_4GC P3
SA076155GC_P3_5GC P3
SA076156GC_P3_1GC P3
SA076157GC_P3_2GC P3
SA076158GC_P3_3GC P3
SA076159GC_P6_6GC P6
SA076160GC_P6_5GC P6
SA076161GC_P6_4GC P6
SA076162GC_P6_1GC P6
SA076163GC_P6_2GC P6
SA076164GC_P6_3GC P6
SA076165GC_P9_6GC P9
SA076166GC_P9_5GC P9
SA076167GC_P9_1GC P9
SA076168GC_P9_4GC P9
SA076169GC_P9_2GC P9
SA076170GC_P9_3GC P9
SA076171M_E18_6M E18
SA076172M_E18_5M E18
SA076173M_E18_1M E18
SA076174M_E18_2M E18
SA076175M_E18_4M E18
SA076176M_E18_3M E18
SA076177M_P0_6M P0
SA076178M_P0_5M P0
SA076179M_P0_4M P0
SA076180M_P0_1M P0
SA076181M_P0_2M P0
SA076182M_P0_3M P0
SA076183M_P3_5M P3
SA076184M_P3_4M P3
SA076185M_P3_6M P3
SA076186M_P3_3M P3
SA076187M_P3_1M P3
SA076188M_P3_2M P3
SA076189M_P6_5M P6
SA076190M_P6_4M P6
SA076191M_P6_6M P6
SA076192M_P6_3M P6
SA076193M_P6_1M P6
SA076194M_P6_2M P6
SA076195M_P9_6M P9
SA076196M_P9_5M P9
SA076197M_P9_3M P9
SA076198M_P9_1M P9
SA076199M_P9_2M P9
SA076200M_P9_4M P9
Showing results 1 to 60 of 60

Collection:

Collection ID:CO001155
Collection Summary:Growth cones (GC) were enriched by sucrose-gradient centrifugation from embryonic and postnatal C57BL/6J mouse brains. Non-GC membranes (M) were collected for comparison.
Sample Type:Brain
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001175
Treatment Summary:As described in the method.

Sample Preparation:

Sampleprep ID:SP001168
Sampleprep Summary:As described in the method.
Extract Storage:-20℃

Combined analysis:

Analysis ID AN001811
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Accela 600
Column Thermo Acclaim C30 (150 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units main area

Chromatography:

Chromatography ID:CH001278
Chromatography Summary:Reversed phase chromatographic separation utilized Accela Autosampler (Thermo), Accela 600 pump (Thermo) and Acclaim C30 column: 3 µm, 2.1x150 mm (Thermo). The column temperature was maintained at 30˚C and tray temperature at 20˚C. Solvent A was composed of 10 mM ammonium acetate (LC-MS grade) in 60:40 methanol:water (LC-MS grade) with 0.2% formic acid (FA; LC-MS grade). Solvent B was composed of 10 mM ammonium acetate with 60:40 methanol:chloroform with 0.2% FA. The flow rate was 260 µL/min and injection volume was 10 µL. The gradient was 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min.
Instrument Name:Thermo Accela 600
Column Name:Thermo Acclaim C30 (150 x 2.1mm,3um)
Column Temperature:30
Flow Gradient:35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min
Flow Rate:260 µL/min
Solvent A:60% methanol/40% water; 0.2% formic acid; 10 mM ammonium acetate
Solvent B:60% methanol/40% chloroform; 0.2% formic acid; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS001671
Analysis ID:AN001811
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:UNSPECIFIED
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