Summary of Study ST001120
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000751. The data can be accessed directly via it's Project DOI: 10.21228/M8M676 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001120 |
| Study Title | Reprogrammed Lipid Metabolism in Bladder Cancer with Cisplatin Resistance |
| Study Summary | This study conducted comprehensive and comparative lipidomic profiling of two isogenic human T24 bladder cell lines, which are characterized as sensitive or resistant to the cisplatin-induced cell apoptosis. |
| Institute | Cedars-Sinai Medical Center |
| Department | Departments of Surgery and Biomedical Sciences |
| Last Name | Kim |
| First Name | Jayoung |
| Address | 8700 Beverly Blvd., Davis 5071 Los Angeles, CA 90048, USA |
| Jayoung.Kim@cshs.org | |
| Phone | 310-423-7168 |
| Submit Date | 2017-04-10 |
| Study Comments | Treatment with ACSS1 inhibitor |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-03-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000751 |
| Project DOI: | doi: 10.21228/M8M676 |
| Project Title: | Lipid Metabolism in Bladder Cancer with Cisplatin Resistance |
| Project Summary: | This study conducted comprehensive and comparative lipidomic profiling of two isogenic human T24 bladder cell lines, which are characterized as sensitive or resistant to the cisplatin-induced cell apoptosis. |
| Institute: | Cedars-Sinai Medical Center |
| Department: | Departments of Surgery and Biomedical Sciences |
| Last Name: | Kim |
| First Name: | Jayoung |
| Address: | 8700 Beverly Blvd., Davis 5071 Los Angeles, CA 90048, USA |
| Email: | Jayoung.Kim@cshs.org |
| Phone: | 310-423-7168 |
Subject:
| Subject ID: | SU001177 |
| Subject Type: | Cell line |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Cell line; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Cell line | Treatment |
|---|---|---|---|
| SA077741 | R-_02 | T24R | No |
| SA077742 | R-_01 | T24R | No |
| SA077743 | R+_02 | T24R | Yes |
| SA077744 | R+_01 | T24R | Yes |
| SA077745 | S-_01 | T24S | No |
| SA077746 | S-_02 | T24S | No |
| SA077747 | S+_01 | T24S | Yes |
| SA077748 | S+_02 | T24S | Yes |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO001171 |
| Collection Summary: | T24 BC cells were obtained and cultured, according to instructions provided by the ATCC. Media was supplemented with 10% fetal bovine serum, 2% glutamine and 1% antibiotics (Invitrogen, Carlsbad, CA). Cells were maintained under a humidified atmosphere of 5% CO2 at 37°C. These cell lines were negative for mycoplasma and all other infectious agents evaluated. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001192 |
| Treatment Summary: | T24 BC cells were characterized in our laboratory as cisplatin resistant BC (T24R, R) or cisplatin sensitive BC cells (T24S, S) to cisplatin-induced apoptosis. |
Sample Preparation:
| Sampleprep ID: | SP001185 |
| Sampleprep Summary: | We separated lipid extracts from proteins and polar hydrophilic small molecules in a way where the lipids are found in the top layer of liquid-liquid separations, rather than in the bottom layer. Decanting the top layer, therefore, ensured that proteins or polar compounds did not contaminate extracts. |
Combined analysis:
| Analysis ID | AN001832 | AN001833 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | normalized_intensity | normalized_intensity |
Chromatography:
| Chromatography ID: | CH001311 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001693 |
| Analysis ID: | AN001832 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
| MS ID: | MS001694 |
| Analysis ID: | AN001833 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | NEGATIVE |
