Summary of Study ST001138
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000703. The data can be accessed directly via it's Project DOI: 10.21228/M8T10P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001138 |
| Study Title | P4HA1 knockdown in the breast cell line MDA231 Gln metabolism (part V) |
| Study Type | isotope tracer |
| Study Summary | Determine the effect on glutamine metabolism of knocking down P4HA1 in the MDA231 cell line. |
| Institute | University of Kentucky |
| Department | Markey Cancer Center |
| Last Name | Xiong |
| First Name | Gaofeng |
| Address | BBSRC361, 741 South Limestone, Lexington, KY 40536, USA |
| gaofeng.xiong@uky.edu | |
| Phone | 000-000-0000 |
| Submit Date | 2019-02-15 |
| Study Comments | For additional comments about the study, like listing a publication. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2019-03-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000703 |
| Project DOI: | doi: 10.21228/M8T10P |
| Project Title: | P4HA1 knockdown in the breast cell line MDA231 |
| Project Summary: | Determine the effect of knocking down P4HA1 in the MDA231 cell line (including alterations to metabolic pathways). |
| Institute: | University of Kentucky |
| Department: | Markey Cancer Center |
| Last Name: | Xu |
| First Name: | Ren |
| Address: | BBSRC361, 741 South Limestone, Lexington, KY 40536, USA |
| Email: | ren.xu2010@uky.edu |
| Phone: | 000-000-0000 |
Subject:
| Subject ID: | SU001202 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | protocol.id |
|---|---|---|
| SA078285 | 1_MDA231_12C14NGln_shCTL_rep1-cells-polar_NMR_A | 12C14NGln_shCTL |
| SA078286 | 5_MDA231_12C14NGln_shP4HA1_rep1-cells-polar_NMR_A | 12C14NGln_shP4HA1 |
| SA078287 | 2_MDA231_13C15NGln_shCTL_rep1-cells-polar_NMR_A | 13C15NGln_shCTL |
| SA078288 | 4_MDA231_13C15NGln_shCTL_rep3-cells-polar_NMR_A | 13C15NGln_shCTL |
| SA078289 | 3_MDA231_13C15NGln_shCTL_rep2-cells-polar_NMR_A | 13C15NGln_shCTL |
| SA078290 | 8_MDA231_13C15NGln_shP4HA1_rep3-cells-polar_NMR_A | 13C15NGln_shP4HA1 |
| SA078291 | 6_MDA231_13C15NGln_shP4HA1_rep1-cells-polar_NMR_A | 13C15NGln_shP4HA1 |
| SA078292 | 7_MDA231_13C15NGln_shP4HA1_rep2-cells-polar_NMR_A | 13C15NGln_shP4HA1 |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO001196 |
| Collection Summary: | Collection and quench of cells in culture |
| Collection Protocol ID: | cell_quench_collection |
| Collection Protocol Filename: | 3A_Cells_Quench_Cell_Fan_20170412.pdf |
| Sample Type: | cultured cells |
Treatment:
| Treatment ID: | TR001217 |
| Treatment Summary: | Cells grown in unlabeled glutamine and infected with empty vector. Cells grown in unlabeled glutamine and infected with shP4HA1 lentivirus. Cells grown in labeled glutamine and infected with empty vector. Cells grown in labeled glutamine and infected with shP4HA1 lentivirus. |
| Treatment Protocol ID: | 12C14NGln_shCTL 12C14NGln_shP4HA1 13C15NGln_shCTL 13C15NGln_shP4HA1 |
Sample Preparation:
| Sampleprep ID: | SP001210 |
| Sampleprep Summary: | Polar extraction from cells. Protein extraction and quantification. |
| Sampleprep Protocol ID: | polar_extraction protein_extraction |
| Sampleprep Protocol Filename: | 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf 4D_17Jun4_Fan_Prot_Quant.pdf |
Analysis:
| Analysis ID: | AN001868 |
| Analysis Type: | NMR |
| Num Factors: | 4 |
| Num Metabolites: | 187 |
| Units: | normalized peak area |
NMR:
| NMR ID: | NM000146 |
| Analysis ID: | AN001868 |
| Instrument Name: | Agilent 600 |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | PRESAT (1H1D) |
| Standard Concentration: | 0.5 mM |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | cryogenic triple resonance HCN |
| NMR Solvent: | D20 |
| NMR Tube Size: | 1.7 mm |
| Shimming Method: | gradient shimming |
| Pulse Sequence: | PRESAT |
| Water Suppression: | presaturation |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 15 celsius |
| Acquisition Time: | 2 s |
| Relaxation Delay: | 4 s |
| Baseline Correction Method: | Bernstein Polynomial |
