Summary of Study ST001168

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000780. The data can be accessed directly via it's Project DOI: 10.21228/M8VH63 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples | Perform analysis on untargeted data
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST001168
Study Title	Alterations in fecal metabolic patterns are associated with atrial fibrillation
Study Summary	Little evidence has been reported in characterizing the fecal alterations in metabolic patterns in atrial fibrillation (AF). We include the result of the global alterations that occur in the intestinal microbiota in a cohort of AF patients and matched controls based on a strategy of metabolomic analyses. Our findings characterize the disordered microbial metabolite profiles in AF.
Institute	Beijing Chaoyang Hospital
Last Name	Zuo
First Name	Kun
Address	8th Gongtinanlu Rd, Chaoyang District, Beijing, China, 100020
Email	zuokun699@163.com
Phone	86-10-15210511744
Submit Date	2019-04-10
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2019-04-17
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000780
Project DOI:	doi: 10.21228/M8VH63
Project Title:	Alterations in fecal metabolic patterns are associated with atrial fibrillation
Project Summary:	Little evidence has been reported in characterizing the fecal alterations in metabolic patterns in atrial fibrillation (AF). We include the result of the global alterations that occur in the intestinal microbiota in a cohort of AF patients and matched controls based on a strategy of metabolomic analyses. Our findings characterize the disordered microbial metabolite profiles in AF.
Institute:	Beijing Chaoyang Hospital
Last Name:	Zuo
First Name:	Kun
Address:	8th Gongtinanlu Rd,, Chaoyang District, Beijing, Beijing, 10020, China
Email:	zuokun699@163.com
Phone:	10-86-15210511744

Subject:

Subject ID:	SU001233
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Group
SA080734	af37	A
SA080735	af9	A
SA080736	af40	A
SA080737	af36	A
SA080738	af39	A
SA080739	af35	A
SA080740	af32	A
SA080741	af33	A
SA080742	af34	A
SA080743	af41	A
SA080744	af42	A
SA080745	af48	A
SA080746	af49	A
SA080747	af50	A
SA080748	af47	A
SA080749	af46	A
SA080750	af43	A
SA080751	af44	A
SA080752	af45	A
SA080753	af31	A
SA080754	af38	A
SA080755	af15	A
SA080756	af16	A
SA080757	af17	A
SA080758	af18	A
SA080759	af14	A
SA080760	af13	A
SA080761	af10	A
SA080762	af11	A
SA080763	af30	A
SA080764	af19	A
SA080765	af12	A
SA080766	af26	A
SA080767	af27	A
SA080768	af29	A
SA080769	af20	A
SA080770	af25	A
SA080771	af28	A
SA080772	af21	A
SA080773	af24	A
SA080774	af22	A
SA080775	af23	A
SA080776	FC29	C
SA080777	FC2	C
SA080778	FC17	C
SA080779	FC14	C
SA080780	FC13	C
SA080781	FC12	C
SA080782	FC9	C
SA080783	FC16	C
SA080784	FA4	C
SA080785	FA9	C
SA080786	FA7	C
SA080787	FA6	C
SA080788	FA13	C
SA080789	FA2	C
SA080790	FA3	C
SA080791	FA12	C
SA080792	FC10	C

Showing results 1 to 59 of 59

Showing results 1 to 59 of 59

Collection:

Collection ID:	CO001227
Collection Summary:	Fecal samples were collected from each participant, immediately frozen at −20 °C, transported on ice to the laboratory and then stored at −80 °C.
Sample Type:	Feces

Treatment:

Treatment ID:	TR001248
Treatment Summary:	To explore how the host metabolic pattern alterations were impacted by the gut microbiota dysbiosis in AF patients, serum samples were collected and analyzed by high-throughput liquid chromatography-mass spectrometry (LC/MS).

Sample Preparation:

Sampleprep ID:	SP001241
Sampleprep Summary:	50 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis.

Sampleprep ID:

SP001241

Sampleprep Summary:

50 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis.

Combined analysis:

Analysis ID	AN001931	AN001932
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Thermo Dionex Ultimate 3000	Thermo Dionex Ultimate 3000
Column	Thermo Hypergod C18 (100 x 4.6mm,3um)	Thermo Hypergod C18 (100 x 4.6mm,3um)
MS Type	ESI	ESI
MS instrument type	Orbitrap	Orbitrap
MS instrument name	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Ion Mode	POSITIVE	NEGATIVE
Units	feature area	feature area

Chromatography:

Chromatography ID:	CH001402
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Thermo Hypergod C18 (100 x 4.6mm,3um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS001787
Analysis ID:	AN001931
Instrument Name:	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100.
Ion Mode:	POSITIVE

MS ID:	MS001788
Analysis ID:	AN001932
Instrument Name:	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100.
Ion Mode:	NEGATIVE