Summary of Study ST001169

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000781. The data can be accessed directly via it's Project DOI: 10.21228/M8QT12 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001169
Study Title	Alterations in serum metabolic patterns are associated with atrial fibrillation
Study Summary	Little evidence has been reported in characterizing the serum alterations in metabolic patterns in atrial fibrillation (AF). We include the result of the global alterations that occur in the intestinal microbiota in a cohort of AF patients and matched controls based on a strategy of metabolomic analyses. Our findings characterize the disordered microbial metabolite profiles in AF.
Institute	Beijing Chaoyang Hospital
Last Name	Zuo
First Name	Kun
Address	8th Gongtinanlu Rd, Chaoyang District, Beijing, China, 100020
Email	zuokun699@163.com
Phone	86-10-15210511744
Submit Date	2019-04-11
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2019-04-17
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR000781
Project DOI:	doi: 10.21228/M8QT12
Project Title:	Alterations in serum metabolic patterns are associated with atrial fibrillation
Project Summary:	Little evidence has been reported in characterizing the serum alterations in metabolic patterns in atrial fibrillation (AF). We include the result of the global alterations that occur in the intestinal microbiota in a cohort of AF patients and matched controls based on a strategy of metabolomic analyses. Our findings characterize the disordered microbial metabolite profiles in AF.
Institute:	Beijing Chaoyang Hospital
Last Name:	Zuo
First Name:	Kun
Address:	8th Gongtinanlu Rd,, Chaoyang District, Beijing, Beijing, 10020, China
Email:	zuokun699@163.com
Phone:	10-86-15210511744

Subject:

Subject ID:	SU001234
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Group
SA080793	afb-34	A
SA080794	afb-35	A
SA080795	afb-33	A
SA080796	afb-31	A
SA080797	afb-30	A
SA080798	afb-36	A
SA080799	afb-32	A
SA080800	afb-38	A
SA080801	afb-42	A
SA080802	afb-13	A
SA080803	afb-41	A
SA080804	afb-40	A
SA080805	afb-39	A
SA080806	afb-29	A
SA080807	afb-37	A
SA080808	afb-17	A
SA080809	afb-19	A
SA080810	afb-16	A
SA080811	afb-15	A
SA080812	afb-14	A
SA080813	afb-28	A
SA080814	afb-20	A
SA080815	afb-18	A
SA080816	afb-26	A
SA080817	afb-27	A
SA080818	afb-24	A
SA080819	afb-23	A
SA080820	afb-21	A
SA080821	afb-22	A
SA080822	B55	C
SA080823	A50	C
SA080824	B63	C
SA080825	B30	C
SA080826	B37	C
SA080827	B24	C
SA080828	B21	C
SA080829	B40	C
SA080830	B4	C
SA080831	B13	C
SA080832	B57	C
SA080833	B11	C
SA080834	B17	C
SA080835	A5	C
SA080836	B49	C
SA080837	B14	C
SA080838	A20	C
SA080839	A19	C
SA080840	A30	C
SA080841	A31	C
SA080842	A49	C
SA080843	A23	C
SA080844	A22	C
SA080845	A9	C
SA080846	A10	C
SA080847	A13	C
SA080848	A6	C
SA080849	A54	C
SA080850	A18	C
SA080851	B60	C
SA080852	B23	C
SA080853	B8	C
SA080854	A33	C
SA080855	A47	C
SA080856	A34	C
SA080857	B9	C

Showing results 1 to 65 of 65

Showing results 1 to 65 of 65

Collection:

Collection ID:	CO001228
Collection Summary:	Blood samples were collected from each participant, immediately frozen at −20 °C, transported on ice to the laboratory and then stored at −80 °C.
Sample Type:	Blood (serum)

Treatment:

Treatment ID:	TR001249
Treatment Summary:	To explore how the host metabolic pattern alterations were impacted by the gut microbiota dysbiosis in AF patients, serum samples were collected and analyzed by high-throughput liquid chromatography-mass spectrometry (LC/MS).

Sample Preparation:

Sampleprep ID:	SP001242
Sampleprep Summary:	The serum samples were thawed at room temperature and 100 μL was pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 300 μL of methanol, and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were vortexed for 30 s and centrifuged at 12000 rpm for 15 minat 4 °C. 200 μL of the supernatant was transferred to a vial for further analysis.

Sampleprep ID:

SP001242

Sampleprep Summary:

The serum samples were thawed at room temperature and 100 μL was pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 300 μL of methanol, and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were vortexed for 30 s and centrifuged at 12000 rpm for 15 minat 4 °C. 200 μL of the supernatant was transferred to a vial for further analysis.

Combined analysis:

Analysis ID	AN001933	AN001934
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Thermo Dionex Ultimate 3000	Thermo Dionex Ultimate 3000
Column	Thermo Hypergod C18 (100 x 4.6mm,3um)	Thermo Hypergod C18 (100 x 4.6mm,3um)
MS Type	ESI	ESI
MS instrument type	Orbitrap	Orbitrap
MS instrument name	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Ion Mode	POSITIVE	NEGATIVE
Units	feature area	feature area

Chromatography:

Chromatography ID:	CH001403
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Thermo Hypergod C18 (100 x 4.6mm,3um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS001789
Analysis ID:	AN001933
Instrument Name:	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100.
Ion Mode:	POSITIVE

MS ID:	MS001790
Analysis ID:	AN001934
Instrument Name:	Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100.
Ion Mode:	NEGATIVE