Summary of Study ST001177
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000789. The data can be accessed directly via it's Project DOI: 10.21228/M8PT1R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001177 |
| Study Title | Analysis of short chain phosphatidylcholine (PC) on the Golgi membrane |
| Study Type | Golgi membrane lipids characterization |
| Study Summary | Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. We have identified that short chain lipids promote membrane properties that are conducive for fission. Here we investigated short chain PCs on Golgi membrane. These findings will advance the understanding of how lipid geometry contributes to membrane deformation needed for vesicle fission. |
| Institute | University of Kentucky |
| Last Name | Morris |
| First Name | Andrew |
| Address | 900 S limestone |
| a.j.morris@uky.edu | |
| Phone | 859-323-3749 |
| Submit Date | 2019-04-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-05-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000789 |
| Project DOI: | doi: 10.21228/M8PT1R |
| Project Title: | Short Chain Lipid Project |
| Project Type: | Untargted analysis |
| Project Summary: | Detect phosphatidylcholine (PC) with short acyl chains on the Golgi membrane |
| Institute: | Brigham and Women's Hospital |
| Department: | Division of Rheumatology, Immunology and Allergy |
| Last Name: | Hsu |
| First Name: | Victor |
| Address: | 60 Fenwood Road, Boston, MA 02115 USA |
| Email: | vhsu@bwh.harvard.edu |
| Phone: | 617-525-1103 |
Subject:
| Subject ID: | SU001243 |
| Subject Type: | Other |
| Subject Species: | Cricetulus griseus |
| Taxonomy ID: | 10029 |
Factors:
Subject type: Other; Subject species: Cricetulus griseus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample Type |
|---|---|---|
| SA081599 | 20180922_sample_01 | Golgi membrane sample |
| Showing results 1 to 1 of 1 |
Collection:
| Collection ID: | CO001237 |
| Collection Summary: | Golgi membrane were prepared from Chinese hamster ovary cell line as described previously. J Cell Biol 159, 69-78 (2002); EMBO J 24, 4133-4143 (2005). |
| Sample Type: | CHO cells |
Treatment:
| Treatment ID: | TR001258 |
| Treatment Summary: | Golgi membrane were prepared as described previously. J Cell Biol 159, 69-78 (2002); EMBO J 24, 4133-4143 (2005). |
Sample Preparation:
| Sampleprep ID: | SP001251 |
| Sampleprep Summary: | 5 mg of Golgi membrane fraction was extracted using acidified CHCl3/methanol (1:2) |
Combined analysis:
| Analysis ID | AN001953 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | ACQUITY BEH C8 column (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH001416 |
| Chromatography Summary: | Lipid extracts were separated on a Waters ACQUITY BEH C8 column (2.1 × 100 mm, 1.7 μm) with the temperature maintained at 40 °C. The flow rate was 250 μL/min, and the mobile phases were consisted of 60:40 water/acetonitrile (A), and 90:10 isopropanol/acetonitrile (B), both containing 10 mM ammonium formate and 0.1% formic acid. The samples were eluted with a linear gradient from 5 % B to 97 % B over 20 min, maintained at 97 % B for 4 min and re-equilibration with 5 % B for 6 min. The sample injection volume was 5 μL |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACQUITY BEH C8 column (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | a linear gradient from 5 % B to 97 % B over 20 min, maintained at 97 % B for 4 min and re-equilibration with 5 % B for 6 min. |
| Flow Rate: | 250 µL/min |
| Solvent A: | 60% water/40% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001808 |
| Analysis ID: | AN001953 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data analysis and accurate mass calculation were performed using the software Xcalibur 4.0 |
| Ion Mode: | POSITIVE |
