Summary of Study ST001179
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000791. The data can be accessed directly via it's Project DOI: 10.21228/M8F97P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001179 |
| Study Title | Metabolomic analysis of skeletal muscle in young and aged mice |
| Study Summary | Sarcopenia is the age-induced, progressive loss of skeletal muscle mass and function, which results in poor muscle performance. To better understand changes in skeletal muscles during sarcopenia, we performed a metabolomic analysis of skeletal muscle in young (8-week-old) and aged (28-month-old) mice using CE-TOFMS. Our data shows that the metabolites including glucose and polyamine metabolism were decreased in aged mice compared with young mice. In addition, neurotransmitter levels were higher in aged mice. |
| Institute | Kyoto Prefectural University |
| Last Name | Kamei |
| First Name | Yasutomi |
| Address | 1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan |
| ran960116@yahoo.co.jp | |
| Phone | +81-75-703-5661 |
| Submit Date | 2019-05-06 |
| Analysis Type Detail | CE-MS |
| Release Date | 2019-07-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000791 |
| Project DOI: | doi: 10.21228/M8F97P |
| Project Title: | Metabolomic analysis of skeletal muscle in young and aged mice |
| Project Summary: | To better understand the metabolic changes in skeletal muscle during aging, we performed a metabolomic analysis of skeletal muscle in young (8-week-old) and aged (28-month-old) mice using CE-TOFMS. |
| Institute: | Kyoto Prefectural University |
| Last Name: | Kamei |
| First Name: | Yasutomi |
| Address: | 1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan |
| Email: | ran960116@yahoo.co.jp |
| Phone: | +81-75-703-5661 |
Subject:
| Subject ID: | SU001245 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wild type |
| Age Or Age Range: | 8-week-old and 28-month-old |
| Weight Or Weight Range: | 25.4 ± 0.2 g for young and 32.0 ± 0.2 g for aged |
| Gender: | Male |
| Animal Animal Supplier: | Shimizu Laboratory Supplies (Kyoto, Japan) |
| Animal Light Cycle: | 12-h light-dark cycle |
| Animal Feed: | CE2 |
| Species Group: | C57BL/6J |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Age |
|---|---|---|
| SA081606 | Old-3 | 28m |
| SA081607 | Old-4 | 28m |
| SA081608 | Old-5 | 28m |
| SA081609 | Old-2 | 28m |
| SA081610 | Old-1 | 28m |
| SA081611 | Young-2 | 8w |
| SA081612 | Young-3 | 8w |
| SA081613 | Young-4 | 8w |
| SA081614 | Young-5 | 8w |
| SA081615 | Young-1 | 8w |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO001239 |
| Collection Summary: | Gastrocnemius muscles were collected and flash-frozen in liquid N2. |
| Sample Type: | Muscle |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001260 |
| Treatment Summary: | No specific treatment to mention. |
Sample Preparation:
| Sampleprep ID: | SP001253 |
| Sampleprep Summary: | Gastrocnemius muscles of 8-week-old male mice (N=5, young group) and 28-month-old male mice (N=5, aged group) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). Frozen muscle specimens were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to CE-TOFMS analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment. |
Combined analysis:
| Analysis ID | AN001956 | AN001957 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | ESI | ESI |
| MS instrument type | TOF | TOF |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | fold | fold |
Chromatography:
| Chromatography ID: | CH001418 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS001811 |
| Analysis ID: | AN001956 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | POSITIVE |
| MS ID: | MS001812 |
| Analysis ID: | AN001957 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
