Summary of Study ST001187

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000799. The data can be accessed directly via it's Project DOI: 10.21228/M8D98R This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001187
Study TitleEffects of selenate and cadmium exposure on the honey bee metabolome (part-I)
Study SummaryWe moved one frame of brood each from five healthy honey bee colonies with marked Italian queens and housed them in a hive body at 35°C and 50% humidity under constant darkness. We then allowed the bees to emerge, mixed the newly emerged workers (NEWs) to randomize their colony of origin and placed NEWs into 13 cm x 10.5 cm x 6.5 cm wire cages equipped with feeders containing 35mL of deionized water and 35mL 50% sucrose. We also provided a pollen patty to each cage of bees consisting of 269g corn syrup, 113g sucrose and 113g of Bee Pro (Mann Lake, Hackensack, MN). To inoculate the newly emerged workers with their “core” microbiome, we collected 50 mL of foragers from the source hives of the NEWs, immobilized the bees at 4°C, aseptically dissected out the abdomens and macerated the whole abdomens in 50% sucrose. We added 1 mL of the resulting slurry to 34 mL of 50% sucrose solution and fed it to the NEWs. We allowed the bees to feed ad libitium on the mixture for two days before replacing the feeders with 50% sucrose. We allowed the bees to feed for three more days to fully establish a microbiome. Once the bees had an established microbiome, we prepared treatment feeding solutions of 50% sucrose (as a no metal/metalloid control), 50% sucrose spiked with 0.6 mg/L sodium selenate or 50% sucrose with 0.24 mg/L cadmium chloride (Alfa Aesar, Ward Hill, MA) and pollen patties spiked with either 6.0 mg/L selenium or 0.46 mg/L cadmium as in Hladun et al 2015. We again allowed the bees to feed ad libitium. We sampled three bees from 13 cages after four days of continuous exposure to the above-mentioned treatments and immediately placed the samples on dry ice, followed by long-term storage at -80 °C.
Institute
University of California, Riverside
Last NameRothman
First NameJason
Address900 University Ave.
Emailjroth002@ucr.edu
Phone9518275817
Submit Date2019-05-31
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Jason Rothman Jason Rothman
https://dx.doi.org/10.21228/M8D98R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000799
Project DOI:doi: 10.21228/M8D98R
Project Title:Untargeted metabolomics of honey bees exposed to selenate or cadmium
Project Summary:Effects of selenate and cadmium exposure on honey bees.
Institute:University of California, Riverside
Last Name:Rothman
First Name:Jason
Address:900 University Ave., Riverside, CA, 91766, USA
Email:jroth002@ucr.edu
Phone:9518275817

Subject:

Subject ID:SU001254
Subject Type:Insect
Subject Species:Apis mellifera
Taxonomy ID:7460

Factors:

Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA082474HBCD-4Cadmium
SA082475HBCD-2Cadmium
SA082476HBCD-1Cadmium
SA082477HBCD-3Cadmium
SA082478HBCT-1Control
SA082479HBCT-2Control
SA082480HBCT-4Control
SA082481HBCT-3Control
SA082482QC-5Pool
SA082483QC-4Pool
SA082484QC-1Pool
SA082485QC-2Pool
SA082486QC-3Pool
SA082487HBSE-3Selenate
SA082488HBSE-1Selenate
SA082489HBSE-2Selenate
SA082490HBSE-4Selenate
SA082491HBSE-5Selenate
Showing results 1 to 18 of 18

Collection:

Collection ID:CO001248
Collection Summary:We sampled three bees from 13 cages after four days of continuous exposure to the treatments and immediately placed the samples on dry ice, followed by long-term storage at -80 °C.
Sample Type:Insect tissue
Storage Conditions:Described in summary

Treatment:

Treatment ID:TR001269
Treatment Summary:Once the bees had an established microbiome, we prepared treatment feeding solutions of 50% sucrose (as a no metal/metalloid control), 50% sucrose spiked with 0.6 mg/L sodium selenate or 50% sucrose with 0.24 mg/L cadmium chloride (Alfa Aesar, Ward Hill, MA) and pollen patties spiked with either 6.0 mg/L selenium or 0.46 mg/L cadmium and allowed the bees to feed ad libitium.

Sample Preparation:

Sampleprep ID:SP001262
Sampleprep Summary:Samples were freeze-dried, homogenized, and extracted with 30:30:20:20 acetonitrile:methanol:water:isopropanol. Samples were then sonicated, vortexed, and centrifuged before analysis.

Combined analysis:

Analysis ID AN001979
Analysis type MS
Chromatography type Normal phase
Chromatography system Waters Acquity I-Class
Column Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Synapt-G2-Si
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH001428
Instrument Name:Waters Acquity I-Class
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B
Flow Rate:250 ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Normal phase

MS:

MS ID:MS001833
Analysis ID:AN001979
Instrument Name:Waters Synapt-G2-Si
Instrument Type:QTOF
MS Type:ESI
MS Comments:We processed the metabolite data (peak picking, alignment, deconvolution, integration, normalization, and spectral matching) with Progenesis Qi software (Nonlinear Dynamics, Durham, NC).
Ion Mode:POSITIVE
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