Summary of Study ST001189

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000801. The data can be accessed directly via it's Project DOI: 10.21228/M84X26 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001189
Study Title1H NMR spectroscopy-based metabolic profiling of Ophiocordyceps sinensis and Cordyceps militaris of water-boiled and 50% ethanol-soaked extracts
Study TypeNMR
Study SummaryIntroduction Ophiocordyceps sinensis, a well-known Chinese complementary herb, is a rare and valuable therapeutic resource. Cordyceps militaris (C. militaris) is a commonly used substitute for O. sinensis. A metabolomic-based approach for exploring the similarities and differences in the metabolites of O. sinensis and C. militaris in water-boiled and 50% ethanol-soaked extracts is of great significance. Objectives To determine a vital role of extraction methodologies in influencing the metabolic composition of herbs, 1HNMR-based profiling was used to characterize the metabolic fingerprints of O. sinensis and C. militaris. Methods To make a distinction between the global metabolite profiling of O. sinensis and C. militaris extracts obtained from either the water-boiled or 50% ethanol-soaked methods, we screened the herbs samples using 1HNMR-based metabolic fingerprints combined with multivariate statistical analysis. Results This study revealed that a total of 43 (82.69% of 52) metabolites were detectable in both O. sinensis and C. militaris. According to the variable importance in projection (VIP) value and p-value from the Mann-Whitney test, 7 metabolites (alanine, aspartate, glutamate, mannitol, ornithine, serine, and trehalose) differed between O. sinensis and C. militaris. Arginine, glucose, putrescine, pyroglutamate, betaine, O-phosphocholine, and xylose differed significantly between the water-boiled and 50% ethanol-soaked methods used to prepare the herb extracts. Conclusion A total of 52 primary metabolites were identified and quantified from O. sinensis and C. militaris samples. The study suggests that a water-boiled extraction is much faster method and strongly recommended over the 50% ethanol-soaked method for both O. sinensis and C. militaris.
Institute
Sun Yat-sen University
Last NameZhong
First NameXin
AddressGuangzhou
Emailzhongxin@mail.sysu.edu.cn
Phone862084112299
Submit Date2019-04-28
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2020-04-28
Release Version1
Xin Zhong Xin Zhong
https://dx.doi.org/10.21228/M84X26
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000801
Project DOI:doi: 10.21228/M84X26
Project Title:1H NMR spectroscopy-based metabolic profiling of Ophiocordyceps sinensis and Cordyceps militaris of water-boiled and 50% ethanol-soaked extracts
Project Type:NMR
Project Summary:Introduction Ophiocordyceps sinensis, a well-known Chinese complementary herb, is a rare and valuable therapeutic resource. Cordyceps militaris (C. militaris) is a commonly used substitute for O. sinensis. A metabolomic-based approach for exploring the similarities and differences in the metabolites of O. sinensis and C. militaris in water-boiled and 50% ethanol-soaked extracts is of great significance. Objectives To determine a vital role of extraction methodologies in influencing the metabolic composition of herbs, 1HNMR-based profiling was used to characterize the metabolic fingerprints of O. sinensis and C. militaris. Methods To make a distinction between the global metabolite profiling of O. sinensis and C. militaris extracts obtained from either the water-boiled or 50% ethanol-soaked methods, we screened the herbs samples using 1HNMR-based metabolic fingerprints combined with multivariate statistical analysis. Results This study revealed that a total of 43 (82.69% of 52) metabolites were detectable in both O. sinensis and C. militaris. According to the variable importance in projection (VIP) value and p-value from the Mann-Whitney test, 7 metabolites (alanine, aspartate, glutamate, mannitol, ornithine, serine, and trehalose) differed between O. sinensis and C. militaris. Arginine, glucose, putrescine, pyroglutamate, betaine, O-phosphocholine, and xylose differed significantly between the water-boiled and 50% ethanol-soaked methods used to prepare the herb extracts. Conclusion A total of 52 primary metabolites were identified and quantified from O. sinensis and C. militaris samples. The study suggests that a water-boiled extraction is much faster method and strongly recommended over the 50% ethanol-soaked method for both O. sinensis and C. militaris.
Institute:Sun Yat-sen University
Last Name:ZHONG
First Name:Xin
Address:Guangzhou
Email:zhongxin@mail.sysu.edu.cn
Phone:862084112299

Subject:

Subject ID:SU001256
Subject Type:Fungi
Subject Species:Ophiocordyceps sinensis;Cordyceps militaris
Taxonomy ID:72228;73501

Factors:

Subject type: Fungi; Subject species: Ophiocordyceps sinensis;Cordyceps militaris (Factor headings shown in green)

mb_sample_id local_sample_id Species Treatment
SA082579B_Yh_1Cordyceps militaris 50% ethanol-soaked
SA082580B_Yh_2Cordyceps militaris 50% ethanol-soaked
SA082581B_Yh_3Cordyceps militaris 50% ethanol-soaked
SA082582B_Yh_4Cordyceps militaris 50% ethanol-soaked
SA082583B_Yh_5Cordyceps militaris 50% ethanol-soaked
SA082584A_Yh_5Cordyceps militaris Boiled water
SA082585A_Yh_4Cordyceps militaris Boiled water
SA082586A_Yh_1Cordyceps militaris Boiled water
SA082587A_Yh_2Cordyceps militaris Boiled water
SA082588A_Yh_3Cordyceps militaris Boiled water
SA082589B_cc_5Ophiocordyceps sinensis 50% ethanol-soaked
SA082590B_cc_4Ophiocordyceps sinensis 50% ethanol-soaked
SA082591B_cc_1Ophiocordyceps sinensis 50% ethanol-soaked
SA082592B_cc_3Ophiocordyceps sinensis 50% ethanol-soaked
SA082593B_cc_2Ophiocordyceps sinensis 50% ethanol-soaked
SA082594A_cc_3Ophiocordyceps sinensis Boiled water
SA082595A_cc_2Ophiocordyceps sinensis Boiled water
SA082596A_cc_5Ophiocordyceps sinensis Boiled water
SA082597A_cc_1Ophiocordyceps sinensis Boiled water
SA082598A_cc_4Ophiocordyceps sinensis Boiled water
Showing results 1 to 20 of 20

Collection:

Collection ID:CO001250
Collection Summary:two species were collected: Ophiocordyceps sinensis and Cordyceps militaris
Sample Type:fungi

Treatment:

Treatment ID:TR001271
Treatment Summary:two treatments: boiled water and 50% ethanol-soaked

Sample Preparation:

Sampleprep ID:SP001264
Sampleprep Summary:The extracts were centrifuged (15 min, 13,000 rpm) at 4oC and 3 mL of supernatant was collected. The supernatant was frozen at -80oC and then dried using a vacuum centrifugal evaporator (CHRiST, Alpha 2-4/LSC, Germany). The dried residues were separately dissolved in 3 mL of distilled water. A 600 µL extract was filtered (Millipore Amicon® ULTRA 3 kDa) and filtrates were collected. 3-(Trimethylsilyl) propanesulfonic acid (50 µL) pre-dissolved in D2O (an internal standard) was subsequently added to 450 µL of the filtrates. Finally, aliquots (480 µL) of each extract were transferred to a 5 mm NMR tube (Norell, Morganton, NC, USA) for NMR analysis.

Analysis:

Analysis ID:AN001981
Analysis Type:NMR
Num Factors:4
Num Metabolites:52
Units:ppm

NMR:

NMR ID:NM000150
Analysis ID:AN001981
Instrument Name:Bruker AV III 600 MHz NMR spectrometer
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600 MHz
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