Summary of Study ST001190

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000802. The data can be accessed directly via it's Project DOI: 10.21228/M8139N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001190
Study TitleSepsis-related metabolic changes in ileum, jejunum, skeletal muscle, liver and lung
Study SummaryRationale: Sepsis is a multi-organ disease affecting the ileum and jejunum (small intestine),liver, skeletal muscle, and lung clinically. Recently, specific alterations in circulating metabolites have been found in patients with sepsis which are thought to contribute to the pathogenesis of disease. The specific metabolic changes in the ileum, jejunum, liver, skeletal muscle, and lung have not previously been investigated. Methods: Live Pseudomonas aeruginosa isolated from a patient was given via IV catheter to pigs to induce severe sepsis. Eighteen hours later, ileum, jejunum, medial gastrocnemius skeletal muscle, liver, and lung were harvested and flash frozen. Tissues were subsequently processed for non-targeted metabolomics analysis using gas chromatography/mass spectrometry. Results: After 18 hours of sepsis, the ileum and the liver demonstrated significant changes in metabolites involved in linoleic acid metabolism, the ileum and lung had significant changes in valine/leucine/isoleucine metabolism, the jejunum, skeletal muscle, and liver had significant changes in arginine/ proline metabolism, and the skeletal muscle and lung had significant changes in aminoacyl-tRNA biosynthesis by pathway analysis. Pathway analysis also identified changes in metabolic pathways unique for different tissues, including changes in the citric acid cycle (jejunum), beta-alanine metabolism (skeletal muscle), and purine metabolism (liver). Conclusion: These findings demonstrate both overlapping metabolic pathways affected in different tissues and those that are unique to others and provide insight into the metabolic changes in sepsis leading to organ dysfunction. This may allow therapeutic interventions that focus on multiple tissues or single tissues once the relationship of the altered metabolites/metabolism to the underlying pathogenesis of sepsis is determined.
Institute
Indiana University School of Medicine
DepartmentIndiana Center for Musculoskeletal Health / Dept. of Pathology & Laboratory Medicine
LaboratoryMultiple Centers
Last NameWillis
First NameMonte
AddressNA
Emailmonte_willis@outlook.com
Phone(984) 999-5431
Submit Date2018-12-03
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2019-06-08
Release Version1
Monte Willis Monte Willis
https://dx.doi.org/10.21228/M8139N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000802
Project DOI:doi: 10.21228/M8139N
Project Title:Sepsis-related metabolic changes in ileum, jejunum, skeletal muscle, liver and lung
Project Type:GC-MS Non-targeted analysis
Project Summary:Identification of metabolic changes in ileum, jejunum, skeletal muscle, liver, and lung after 18 hours of continuous IV Pseudomonas auriginosa induced sepsis.
Institute:Indiana University School of Medicine
Department:Indiana Center for Musculoskeletal Health / Dept. of Pathology & Laboratory Medicine
Laboratory:Multiple Centers
Last Name:Willis
First Name:Monte
Address:NA
Email:monte_willis@outlook.com
Phone:(984) 999-5431
Funding Source:NIH

Subject:

Subject ID:SU001257
Subject Type:Animal
Subject Species:Sus scrofa
Taxonomy ID:9823

Factors:

Subject type: Animal; Subject species: Sus scrofa (Factor headings shown in green)

mb_sample_id local_sample_id Tissue Challenge
SA082599I-15Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082600I-14Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082601I-13Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082602I-17Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082603I-21Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082604I-1Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082605I-22Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082606I-12Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082607I-20Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082608I-19Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082609I-3Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082610I-6Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082611I-10Ileum Vascular catherization/Pseudomonas aeruginosa infusion
SA082612I-4Ileum Vascular catherization/Saline infusion
SA082613I-2Ileum Vascular catherization/Saline infusion
SA082614I-11Ileum Vascular catherization/Saline infusion
SA082615I-5Ileum Vascular catherization/Saline infusion
SA082616I-16Ileum Vascular catherization/Saline infusion
SA082617I-8Ileum Vascular catherization/Saline infusion
SA082618I-9Ileum Vascular catherization/Saline infusion
SA082619I-7Ileum Vascular catherization/Saline infusion
SA082620I-18Ileum Vascular catherization/Saline infusion
SA082621J-76Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082622J-86Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082623J-78Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082624J-80Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082625J-88Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082626J-85Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082627J-82Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082628J-69Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082629J-68Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082630J-67Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082631J-74Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082632J-71Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082633J-73Jejunum Vascular catherization/Pseudomonas aeruginosa infusion
SA082634J-84Jejunum Vascular catherization/Saline infusion
SA082635J-87Jejunum Vascular catherization/Saline infusion
SA082636J-70Jejunum Vascular catherization/Saline infusion
SA082637J-75Jejunum Vascular catherization/Saline infusion
SA082638J-83Jejunum Vascular catherization/Saline infusion
SA082639J-77Jejunum Vascular catherization/Saline infusion
SA082640J-72Jejunum Vascular catherization/Saline infusion
SA082641J-79Jejunum Vascular catherization/Saline infusion
SA082642J-81Jejunum Vascular catherization/Saline infusion
SA082643Li-36Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082644Li-32Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082645Li-38Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082646Li-42Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082647Li-44Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082648Li-41Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082649Li-34Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082650Li-27Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082651Li-25Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082652Li-24Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082653Li-23Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082654Li-30Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082655Li-29Liver Vascular catherization/Pseudomonas aeruginosa infusion
SA082656Li-31Liver Vascular catherization/Saline infusion
SA082657Li-43Liver Vascular catherization/Saline infusion
SA082658Li-40Liver Vascular catherization/Saline infusion
SA082659Li-26Liver Vascular catherization/Saline infusion
SA082660Li-35Liver Vascular catherization/Saline infusion
SA082661Li-33Liver Vascular catherization/Saline infusion
SA082662Li-28Liver Vascular catherization/Saline infusion
SA082663Li-37Liver Vascular catherization/Saline infusion
SA082664Li-39Liver Vascular catherization/Saline infusion
SA082665Lu-103Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082666Lu-101Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082667Lu-GhostLung Vascular catherization/Pseudomonas aeruginosa infusion
SA082668Lu-98Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082669Lu-109Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082670Lu-108Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082671Lu-105Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082672Lu-93Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082673Lu-91Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082674Lu-90Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082675Lu-89Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082676Lu-96Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082677Lu-95-rerun2Lung Vascular catherization/Pseudomonas aeruginosa infusion
SA082678Lu-97Lung Vascular catherization/Saline infusion
SA082679Lu-110Lung Vascular catherization/Saline infusion
SA082680Lu-107Lung Vascular catherization/Saline infusion
SA082681Lu-92Lung Vascular catherization/Saline infusion
SA082682Lu-102Lung Vascular catherization/Saline infusion
SA082683Lu-99Lung Vascular catherization/Saline infusion
SA082684Lu-94Lung Vascular catherization/Saline infusion
SA082685Lu-104Lung Vascular catherization/Saline infusion
SA082686Lu-106Lung Vascular catherization/Saline infusion
SA082687M-60Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082688M-56Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082689M-58Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082690M-66Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082691M-54Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082692M-64Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082693M-63Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082694M-49Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082695M-47Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082696M-51Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082697M-46Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
SA082698M-45Skeletal Muscle (Gastrocnemius) Vascular catherization/Pseudomonas aeruginosa infusion
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Collection:

Collection ID:CO001251
Collection Summary:Tissue was harvested and flash frozen in a liquid nitrogen cooled
Sample Type:Ileum;Lung;Liver;Jejunum;Skeletal muscle

Treatment:

Treatment ID:TR001272
Treatment Summary:Fraction of cardiac tissue weighed (~25 mg wet weight), then the finely cut up tissue quickly added to fresh pre-made buffer (50 % acetyl-nitrile, 50 % water, 0.3% formic acid) at a standard concentration of 25 mg/475 microlieters (mcl) buffer then fully homogenized on ice for 20 seconds and placed on dry ice/stored at -80C

Sample Preparation:

Sampleprep ID:SP001265
Sampleprep Summary:The samples were crash deprotonized by methanol precipitation and spiked with D27-deuterated myristic acid (D27-C14:0) as an internal standard for retention-time locking and dried. The trimethylsilyl-D27-C14:0 standard retention time (RT) was set at 16.727 min. Reactive carbonyls were stabilized at 50C with methoxyamine hydrochloride in dry pyridine. Metabolites were made volatile with TMS groups using N-methyl-N-(trimethylsilyl) trifluoroacetamide or MSTFA with catalytic trimethylchlorosilane at 50C.

Combined analysis:

Analysis ID AN001982
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890 N GC
Column Agilent DB5-MS (15m x 0.25mm,0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units Peak values (Log transformed)

Chromatography:

Chromatography ID:CH001430
Chromatography Summary:GC/MS methods follow previous studies using a 6890 N GC connected to a 5975 Inert single quadrupole MS (Agilent Technologies, Santa Clara, CA) (Bonikos et al. 1975; Fiehn 2008; Kind et al. 2009). The two wall-coated, open-tubular GC columns connected in series are both from J&W/Agilent (part 122-5512), DB5-MS, 15 meters in length, 0.25 mm in diameter, with an 0.25-l m luminal film. Positive ions generated with conventional electron-ionization at 70 eV are scanned broadly from 600 to 50 m/z in the detector throughout the 45 min cycle time.
Instrument Name:Agilent 6890 N GC
Column Name:Agilent DB5-MS (15m x 0.25mm,0.25um)
Chromatography Type:GC

MS:

MS ID:MS001835
Analysis ID:AN001982
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:NA
Ion Mode:POSITIVE
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