Summary of Study ST001208
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000812. The data can be accessed directly via it's Project DOI: 10.21228/M8QM44 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001208 |
| Study Title | Characterization of feces in Atrial Fibrillation (AF) patients |
| Study Summary | Atrial Fibrillation (AF), an abnormal heart rhythm characterized by the rapid and irregular beating of the atria, is the most common arrhythmia with heavy global burdens. The present project aimed to characterized the feature of metabolites in feces of AF patients by using LC-MS. |
| Institute | Capital Medical University |
| Last Name | Zuo |
| First Name | Kun |
| Address | beijing chaoyang hospital |
| zuokun699@163.com | |
| Phone | 010-85231937 |
| Submit Date | 2018-12-27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-07-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000812 |
| Project DOI: | doi: 10.21228/M8QM44 |
| Project Title: | Characterization of feces in Atrial Fibrillation (AF) patients |
| Project Summary: | Atrial Fibrillation (AF), an abnormal heart rhythm characterized by the rapid and irregular beating of the atria, is the most common arrhythmia with heavy global burdens. The present project aimed to characterized the feature of metabolites in feces of AF patients. |
| Institute: | Capital Medical University |
| Last Name: | Zuo |
| First Name: | Kun |
| Address: | beijing chaoyang hospital |
| Email: | zuokun699@163.com |
| Phone: | +861085231937 |
Subject:
| Subject ID: | SU001275 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factors |
|---|---|---|
| SA084822 | AF37 | AF |
| SA084823 | AF38 | AF |
| SA084824 | AF40 | AF |
| SA084825 | AF36 | AF |
| SA084826 | AF39 | AF |
| SA084827 | AF35 | AF |
| SA084828 | AF32 | AF |
| SA084829 | AF33 | AF |
| SA084830 | AF34 | AF |
| SA084831 | AF41 | AF |
| SA084832 | AF42 | AF |
| SA084833 | AF48 | AF |
| SA084834 | AF49 | AF |
| SA084835 | AF50 | AF |
| SA084836 | AF47 | AF |
| SA084837 | AF46 | AF |
| SA084838 | AF43 | AF |
| SA084839 | AF44 | AF |
| SA084840 | AF45 | AF |
| SA084841 | AF31 | AF |
| SA084842 | AF30 | AF |
| SA084843 | AF15 | AF |
| SA084844 | AF16 | AF |
| SA084845 | AF17 | AF |
| SA084846 | AF18 | AF |
| SA084847 | AF14 | AF |
| SA084848 | AF13 | AF |
| SA084849 | AF10 | AF |
| SA084850 | AF11 | AF |
| SA084851 | AF12 | AF |
| SA084852 | AF19 | AF |
| SA084853 | AF20 | AF |
| SA084854 | AF26 | AF |
| SA084855 | AF27 | AF |
| SA084856 | AF28 | AF |
| SA084857 | AF29 | AF |
| SA084858 | AF25 | AF |
| SA084859 | AF24 | AF |
| SA084860 | AF21 | AF |
| SA084861 | AF22 | AF |
| SA084862 | AF23 | AF |
| SA084863 | AF9 | AF |
| Showing results 1 to 42 of 42 |
Collection:
| Collection ID: | CO001269 |
| Collection Summary: | Fresh stool samples were collected from each participant, immediately frozen at −20 °C, transported on ice to the laboratory and then stored at −80 °C. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR001290 |
| Treatment Summary: | We enrolled participants consisting of patients with AF. |
Sample Preparation:
| Sampleprep ID: | SP001283 |
| Sampleprep Summary: | 20 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis. |
Combined analysis:
| Analysis ID | AN002010 | AN002011 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Hypersil Gold C18 | Hypersil Gold C18 |
| MS Type | ESI | ESI |
| MS instrument type | Ion trap | Ion trap |
| MS instrument name | Thermo Deca LCQ | Thermo Deca LCQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pg | pg |
Chromatography:
| Chromatography ID: | CH001454 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Hypersil Gold C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001863 |
| Analysis ID: | AN002010 |
| Instrument Name: | Thermo Deca LCQ |
| Instrument Type: | Ion trap |
| MS Type: | ESI |
| MS Comments: | Compound Discoverer 2.0 software (Thermo) |
| Ion Mode: | POSITIVE |
| MS ID: | MS001864 |
| Analysis ID: | AN002011 |
| Instrument Name: | Thermo Deca LCQ |
| Instrument Type: | Ion trap |
| MS Type: | ESI |
| MS Comments: | Compound Discoverer 2.0 software (Thermo) |
| Ion Mode: | NEGATIVE |
